%0 Journal Article %J Knowledge & Management of Aquatic Ecosystems %D 2021 %T A comment on “Morphologic and genetic characterization of Corsican and Sardinian trout with comments on Salmotaxonomy” by Delling et al. (2020): protected Tyrrhenian trouts must be named %A Denys, Gaël %B Knowledge & Management of Aquatic Ecosystems %P 6 %8 Jan-01-2021 %G eng %U https://www.kmae-journal.org/10.1051/kmae/2021006 %N 422 %! Knowl. Manag. Aquat. Ecosyst. %R 10.1051/kmae/2021006 %0 Journal Article %J Cybium %D 2021 %T The complete mitochondrial genome of Barbatula quignardi (Băcescu-Meşter, 1967) (Teleostei, Nemacheilidae) %A Gauliard, Camille %A Denys, Gaël %A Perea, Silvia %A Dettai, Agnès %X The complete mitochondrial DNA sequence of Barbatula quignardi (Teleostei, Nemacheilidae) was sequenced from a museum voucher caught in its type locality (Lez River). The sequence was 16,641 bp in length, consisted of 13 protein-coding genes, 22 transfer RNA genes including 2 tRNA-Leu and 2 tRNA-Ser, 2 ribosomic RNA genes and the control region. Intergenic space and overlapping gene sequences were found. The base composition of the whole mtDNA was 28.7% A, 26.2% T, 27.2% C and 17.9% G. %B Cybium %V 45 %P 39-42 %G eng %N 1 %0 Journal Article %J Organisms Diversity & Evolution %D 2021 %T Global systematic diversity, range distributions, conservation and taxonomic assessments of graylings (Teleostei: Salmonidae; Thymallus spp.)Abstract %A Weiss, Steven J. %A Gonçalves, Duarte V. %A Secci-Petretto, Giulia %A Englmaier, Gernot K. %A Gomes-Dos-Santos, André %A Denys, Gaël %A Persat, Henri %A Antonov, Alexander %A Hahn, Christoph %A Taylor, Eric B. %A Froufe, Elsa %B Organisms Diversity & Evolution %V 21 %P 25 - 42 %8 Jan-03-2021 %G eng %U http://link.springer.com/10.1007/s13127-020-00468-7 %N 1 %! Org Divers Evol %R 10.1007/s13127-020-00468-7 %0 Journal Article %J Cahiers de Biologie Marine %D 2021 %T Observations of Sea Lampreys, attached to Humpback whales, off Saint Pierre and Miquelon archipelago (Northwestern Atlantic) %A Urtizberea, Frank %A Detcheverry, Joël %A Denys, Gaël %X We report the two first observations of Sea Lampreys Petromyzon marinus attached to Humpback whales Megaptera novaeangliae during two whales surveys by boat the 5th September 2012 and 8th July 2020. Their size and marbled coloration certified their species identification. However, the lack of scarring does not allow to affirm a parasite/host relationship. These observations are also the first occurrences of this lamprey species in the exclusive economic zone of the French territory of Saint Pierre and Miquelon archipelago, and will be helpful for French managers. %B Cahiers de Biologie Marine %V 62 %P 77-81 %G eng %0 Journal Article %J Annales de Limnologie - International Journal of Limnology %D 2021 %T Revalidation of the Spanish stone loach Barbatula hispanica (Lelek, 1987) (Teleostei, Nemacheilidae) according to morphological and mitochondrial data %A Denys, Gaël %A Perea, Silvia %A Dettai, Agnès %A Gauliard, Camille %A Persat, Henri %A Doadrio, Ignacio %B Annales de Limnologie - International Journal of Limnology %V 57 %P 10 %8 Jan-01-2021 %G eng %U https://www.limnology-journal.org/10.1051/limn/2021007 %! Ann. Limnol. - Int. J. Lim. %R 10.1051/limn/2021007 %0 Journal Article %J eLife %D 2021 %T The rise and fall of the ancient northern pike master sex-determining gene %A Pan, Qiaowei %A Feron, Romain %A Jouanno, Elodie %A Darras, Hugo %A Herpin, Amaury %A Koop, Ben %A Rondeau, Eric %A Goetz, Frederick W %A Larson, Wesley A %A Bernatchez, Louis %A Tringali, Mike %A Curran, Stephen S %A Saillant, Eric %A Denys, Gaël %A von Hippel, Frank A %A Chen, Songlin %A López, J Andrés %A Verreycken, Hugo %A Ocalewicz, Konrad %A Guyomard, Rene %A Eche, Camille %A Lluch, Jerome %A Roques, Celine %A Hu, Hongxia %A Tabor, Roger %A DeHaan, Patrick %A Nichols, Krista M %A Journot, Laurent %A Parrinello, Hugues %A Klopp, Christophe %A Interesova, Elena A %A Trifonov, Vladimir %A Schartl, Manfred %A Postlethwait, John %A Guiguen, Yann %B eLife %V 10 %8 Apr-01-2023 %G eng %U https://elifesciences.org/articles/62858 %R 10.7554/eLife.62858 %0 Journal Article %J Cybium %D 2020 %T The complete mitochondrial genome of Thymallus thymallus (Linnaeus, 1758) (Actinopterygii, Salmonidae) obtained by long range PCRs and double multiplexing %A Denys, Gaël %A Secci-Petretto, Giulia %A Gomes-Dos-Santos, André %X Le génome mitochondrial complet de Thymallus thymallus (Actinopterygii, Salmonidae) provenant d’un voucher enregistré en collection et capturé dans l’Ain (bassin du Rhône) a été séquencé. La séquence d’une longueur de 16 660 pb, contient 13 gènes codants, 22 ARN de transfert dont 2 ARNt-Leu et 2 ARNt-Ser, 2 gènes ARN ribosomiques et la région de contrôle. Un espace intergénique et des séquences se chevauchant ont été trouvés. La composition des bases du mitogénome est de 27,5% A, 27,9% T, 27,1% C et 17,5% G. Ce mitogénome a été séquencé pour moins de 20 euros. %B Cybium %V 44 %P 91-94 %G eng %N 2 %0 Book %D 2020 %T Les poissons d’eau douce de France %A Philippe Keith %A Poulet, Nicolas %A Denys, Gaël %A Changeux Thomas %A Eric Feunteun %A Persat, Henri %7 2nde %I Biotope Editions, Mèze ; Muséum national d’Histoire naturelle, Paris (collection Inventaires et biodiversité) %C Paris %P 704 %G eng %0 Journal Article %J Naturae %D 2020 %T Premier signalement en France du Barbeau de Graells Luciobarbus graellsii (Steindachner, 1866) (Actinopterygii, Cypriniformes) %A MASSEBOEUF, Fabrice %A Doadrio, Ignacio %A Denys, Gaël %B Naturae %8 Sep-12-2020 %G eng %U http://sciencepress.mnhn.fr/fr/periodiques/naturae/2020/15 %N 15 %! Naturae %R 10.5852/naturae2020a15 %0 Journal Article %J Cybium %D 2020 %T Revision of Phoxinus in France with the description of two new species (Teleostei, Leuciscidae) %A Denys, Gaël %A Dettai, Agnès %A Persat, Henri %A Daszkiewicz, Piotr %A Hautecoeur, Mélyne %A Philippe Keith %X French minnows of the genus Phoxinus are revised based on molecular data (COI and 12S rDNA markers), morphological characters and nuptial colouration patterns. The results delineate six groups of populations, which are recognised as species. Phoxinus phoxinus is found in eastern France in the lower and middle Rhine and Seine drainages. Phoxinus bigerri occurs in the Adour drainage and populations from the Lake Geneva basin and the upper Rhine drainage are identified as Phoxinus csikii. Phoxinus dragarum, new species, from the Garonne drainage, is distinguished by its unique nuptial colour pattern (green stripe in Z3 reaching up to the anal fin base and Z4 greenish yellow in male; Z4 green-ochre and black line below in Z5 reaching from the pectoral fin bases to the anal fin base in female), and by an incomplete lateral line generally reaching to a point in front of the anal fin base or just shortly beyond the origin of the last anal fin ray. Phoxinus fayollarum, new species, from the Loire drainage, is distinguished by its unique nuptial colour pattern (green bars in Z3 and Z4, Z4 bright yellow, and belly red in male; green stripe in Z3, Z4 yellow-pinkish, and red spots at the pectoral, pelvic and anal fin base in female). Minnows of the Rhône drainage are identified as Phoxinus septimaniae. We consider Pisciculus varius and Phoxynus montanus as nomen oblitum and Phoxinus septimaniae as nomen protectum, and Leuciscus obtusus as incertae sedis within Leuciscidae. %B Cybium %V 44 %P 205-237 %G eng %N 3 %0 Conference Proceedings %B SIBIC 2020 %D 2020 %T Upstream as a sanctuary for the French populations of catalan chub Squalius laietanus (Teleostei , Leuciscidae) threatened by the allochtonous European chub S. cephalus %A Rose, Morgane %A Dettai, Agnès %A Lefèbvre, Stéphane %A Blanc, Laurence %A Poulet, Nicolas %A Baudier, Olivier %A Hautecoeur, Mélyne %A Denys, Gaël %X In the Pyrénées-Orientales department (France), three Mediterranean catchments (Agly, Têt and Tech) are subject to high variations in water levels during Cevenol-type events. In order to fight against the effects of these floods, dams and wiers were built along these rivers with consequences on aquatic fauna, eliminating the ecological continuum. The chub Squalius is a potamodromous fish occurring from the salmonids stream to close to the estuaries. Moreover, whereas the European chub S. cephalus was thought to be represented by a single species in France, another species, endemic to Catalonia, was described: the Catalan chub S. laietanus. However, S. cephalus was also introduced in the catchments of this area with possibly threats of hybridization. Samples from eight populations of Squalius spp coming from these rivers were genetically identified using a DNA Barcoding approach with a mitochondrial (COI) and a nuclear (RAG1) markers. Our results confirm the presence of both species in the three drainages as well as their hybridization, threatening S. laietanus by hybridization as in 70% of Mediterrannean endemic species. There is also a downstream-upstream gradient in the presence of S. cephalus in the Têt and Tech catchments. S. laietanus seems less impacted by S. cephalus in headwaters than downstream. One of the explanations would be the numerous weirs between these areas. If the negative impacts of the absence of ecological continuum on the ichthyofauna are known and cannot be denied, these weirs might form in this case a barrier against the presence of S. cephalus in headwaters. As a consequence, S. laietanus has been evaluated as EN in the last French UICN Red List published in 2019. While the ecological parameters still need to be examined, our study brings additional information for better suited conservation actions regarding the ecological continuum. %B SIBIC 2020 %G eng %0 Journal Article %J Cybium %D 2019 %T Barbatula leoparda (Actinopterygii, Nemacheilidae), a new endemic species of stone loach of French Catalonia %A Gauliard, Camille %A Dettai, Agnès %A Persat, Henri %A Philippe Keith %A Denys, Gaël %K Barbatula leopard %K French Catalonia %K Freshwater %K Nemacheilidae %K New species %X

This study described a new stone loach species in France, Barbatula leoparda, which is endemic to French Catalonia (Têt and Tech river drainages). Seven specimens were compared to 49 specimens of B. barbatula (Linnaeus, 1758) and 71 specimens of B. quignardi (Băcescu-Meşter, 1967). This new species is characterized by the presence of blotches on the belly and the jugular area in individuals longer than 47 mm SL and by a greater interorbital distance (35.5 to 41.8% of the head length). We brought moreover the sequence of two mitochondrial markers (COI and 12S, respectively 652 and 950 bp) of the holotype, which are well distinct from all other species, for molecular identifications. This discovery is important for conservation.

%B Cybium %V 43 %P 169-177 %G eng %N 2 %0 Journal Article %J Cybium %D 2019 %T Cottus petiti Bǎcescu & Bǎcescu-Meşter, 1964 (Cottidae) %A Lefèbvre, Stéphane %A Richard, Sylvain %A Beaudou, Domninique %A Denys, Gaël %B Cybium %V 43 %P 215-216 %G eng %N 3 %0 Journal Article %J Frontiers in Marine Science %D 2019 %T Does Loire grayling represent a distinct species ? %A Persat, Henri %A Denys, Gaël %A Froufe, Elsa %A Secci-Petretto, Giulia %A Weiss, Steven Weiss %B Frontiers in Marine Science %V 6 %8 Jan-01-2019 %G eng %U http://www.frontiersin.org/Community/AbstractDetails.aspx?ABS_DOI=10.3389%2fconf.fmars.2019.07.00070 %! Front. Mar. Sci. %R 10.3389/conf.fmars.2019.07.00070 %0 Journal Article %J Cybium %D 2019 %T First record of Phoxinus csikii Hankó, 1922 (Actinopterygii, Cypriniformes) in France %A Denys, Gaël %A Manné, Sébastien %X Une identification moléculaire par barcoding (marqueur du COI, 651 pb) a été réalisée sur un vairon capturé dans le Rupt de Mad à Essey-et-Maizerais (bassin du Rhin/Moselle). Ainsi, ce spécimen appartient à l’espèce récemment revalidée Phoxinus csikii Hankó, 1922. Il s’agit ainsi du premier signalement de cette espèce en France, et de la quatrième espèce de vairon connue dans ce pays. %B Cybium %V 43 %P 199-202 %G eng %N 2 %0 Journal Article %J Frontiers in Marine Science %D 2019 %T Minnow, Minnow, new and old, who is the fairest of them all: the taxonomical review of the French minnows Phoxinus spp. (Actinopterygii, Leuciscidae) %A Denys, Gaël %A Dettai, Agnès %A Persat, Henri %A Hautecoeur, Mélyne %A Philippe Keith %B Frontiers in Marine Science %V 6 %8 Jan-01-2019 %G eng %U http://www.frontiersin.org/Community/AbstractDetails.aspx?ABS_DOI=10.3389%2fconf.fmars.2019.07.00102 %! Front. Mar. Sci. %R 10.3389/conf.fmars.2019.07.00102 %0 Journal Article %J Cybium %D 2019 %T Morphological traits allow distinguishing their hybrids from the Northern pike, Esox lucius, and the Aquitanian pike, Esox aquitanicus (Actinopterygii, Esociformes) %A Jeanroy, Clara %A Denys, Gaël %X Le brochet aquitain Esox aquitanicus Denys, Dettai, Persat, Hautecoeur & Keith, 2014 est une espèce endémique du Sud-Ouest de la France. Il est menacé par l’hybridation avec le brochet commun Esox lucius Linnaeus, 1758. Jusqu’à présent, seul l’outil moléculaire permettait la détermination des hybrides. Le but de cette étude est de trouver des caractères morphologiques pour caractériser les hybrides des deux espèces parentales à partir des trois critères distinguant l’E. aquitanicus de l’E. lucius : le patron de coloration du corps, le nombre d’écailles sur la ligne latérale et la longueur du museau. Nos résultats ont démontré que les spécimens hybrides pouvaient être discriminés d’E. lucius par un museau plus court et un plus faible nombre d’écailles sur la ligne latérale, et d’E. aquitanicus seulement par un plus grand nombre d’écailles sur la ligne latérale. Le patron de coloration du corps des deux espèces est rencontré chez les hybrides. Ainsi, le meilleur caractère pour identifier les hybrides est le nombre d’écailles sur la ligne latérale ; il est intermédiaire comparé aux espèces parentales (114 à 119 vs. 104 à 107 pour E. aquitanicus et (115)120 à 136(146) pour E. lucius). Il sera utile pour les gestionnaires en conservation du brochet aquitain. Néanmoins, les résultats de cette étude nécessitent d’être confirmés avec un plus grand échantillonnage en aquaculture. %B Cybium %V 43 %P 227-232 %G eng %N 3 %0 Journal Article %J Cybium %D 2019 %T A third European species of grayling (Actinopterygii, Salmonidae), endemic to the Loire River basin (France), Thymallus ligericus n. sp. %A Persat, Henri %A Weiss, Steven J. %A Froufe, Elsa %A Secci-Petretto, Giulia %A Denys, Gaël %X Loire grayling was already known to belong to a distinct lineage, compared to other European population, according to molecular data (enzymatic polymorphism, mtDNA sequencing and microsatellites). In this paper, we consider this lineage as a new species, Thymallus ligericus n. sp., which is endemic to the Loire drainage (France). Compared with the other species, T. ligericus n. sp. is characterized by a more elongated body, the presence of a pointed snout and a strait or convex snout profile, a more inferior mouth with a transversal aperture and a fleshier upper lip, usually more than 50 to several hundreds black dots on the flanks, a shorter head (20.0-23.7% SL), smaller occipital and orbital depths of the head (54.8-71.7% HL and 43.5-53.4% HL, respectively) and a shorter horizontal eye diameter (22.6-26.3% HL). %B Cybium %V 43 %P 233-238 %G eng %N 3 %0 Journal Article %J Revue scientifique Bourgogne-Franche-Comté Nature %D 2019 %T Une nouvelle espèce de poisson en Bourgogne-Franche-Comté : le goujon occitan (Gobio occitaniae) %A Denys, Gaël %A Besson, Sylvain %B Revue scientifique Bourgogne-Franche-Comté Nature %V 30-2019 %P 78-79 %G eng %0 Journal Article %J J Zool Syst Evol Res %D 2018 %T Genetic and morphological discrimination of three species of ninespined stickleback Pungitius spp. (Teleostei, Gasterosteidae) in France with the revalidation of Pungitius vulgaris (Mauduyt, 1848) %A Denys, Gaël %A Persat, Henri %A Dettai, Agnès %A Geiger Mathias %A Freyhof, J %A Fesquet, J %A Philippe Keith %K France %K Integrative taxonomy %K mitochondrial DNA COI %K Pungitius %K RNF213 %X

The taxonomy of French ninespined sticklebacks (Pungitius spp.) has long been controversial. To clarify the taxonomy in this group, we use mitochondrial (COI) and nuclear  (RNF213) sequence markers, as well as morphological data. In France, both genetic markers discriminate three evolutionary lineages. Morphological analysis on fresh and type specimens supports the different lineages and the existence of three species in France. Pungitius pungitius, occurring in the North of France and Rhone basin, is characterized by specimens longer than 35 mm SL, by a flat head with a straight or slightly concave snout, typically 9–10 dorsal spines, 10–11 dorsal soft rays, 9–10 anal soft rays, 0–12 scutes on the caudal peduncle with a keel reaching the last anal-fin ray, longer pelvic fin, post-dorsal and caudal peduncle lengths, and a slim caudal peduncle (caudal peduncle depth/ length 11.8%–21.9%). Pungitius laevis, occurring in France, in the English Channel basins and Loire drainage, differs from the other species by a head rounded with concave snout in specimens longer than 35 mm SL, accentuating the impression of fleshy lips, 0–4 scutes on the caudal peduncle and a higher caudal peduncle depth/length ratio (15.7%– 34.5%). Finally, Pungitius vulgaris, endemic to the Vienne River and rivers of south-western France as far north as the Garonne estuary, is differentiated by a rounded head with a straight or slightly convex snout, the absence of scutes on the caudal peduncle and by having 11 pectoral-fin rays. Our data confirm the existence of a hybridization zone in the North of France between P. pungitius and P. laevis. As a result, Pungitius lotharingus is invalid, as it was described based on hybrid specimens. A lectotype for P. laevis was designated because the syntypes included hybrids. This revision provides new perspectives for evolutionary biology studies and will have consequences for Pungitius conservation in France.

%B J Zool Syst Evol Res %V 2017 %P 1–25 %G eng %0 Journal Article %J Cybium %D 2018 %T Presence of larvae of lampreys, Lampetra sp. (Cephalaspidomorphi, Petromyzontiformes), in a French Catalan basin %A Arsento, Rémy %A Richarte, Kévin %A Fonteneau, André %A Denys, Gaël %X Des ammocètes de lamproies ont été récemment capturées pour la première fois dans un cours d’eau de Catalogne française, la Têt. Les identifications morphologique et moléculaire ont démontré que ces ammocètes appartiennent au complexe [Lampetra fluviatilis / Lampetra planeri]. Les lamproies et leurs frayères étant protégées en France, cette étude aura une incidence sur la gestion du cours d’eau de la Têt. %B Cybium %V 42 %P 216-218 %G eng %N 2 %0 Journal Article %J Frontiers in Marine Science %D 2015 %T The freshwater fish fauna history between Rhine and Ebro: general considerations and comments from on-going developments in fish taxonomy. %A Persat, Henri %A Denys, Gaël %A Dettai, Agnès %A Philippe Keith %B Frontiers in Marine Science %V 2 %G eng %N 2015 %0 Journal Article %J Cybium %D 2015 %T Invalidity of Gasterosteus gymnurus (Cuvier, 1829) (Actinopterygii, Gasterosteidae) according to integrative taxonomy. %A Denys, Gaël %A Geiger Mathias %A Persat, Henri %A Philippe Keith %A Dettai, Agnès %K Cytochrome C %K Gasterosteidae Gasterosteus aculeatus Gasterosteus gymnurus Gasterosteus islandicus %K Integrative taxonomy %K oxidase subunit 1 %B Cybium %V 39 %P 37-45 %G eng %N 1 %0 Journal Article %J Comptes Rendus Biologies %D 2014 %T Morphological and molecular evidence of three species of pikes Esox spp. (Actinopterygii, Esocidae) in France, including the description of a new species %A Denys, Gaël %A Dettai, Agnès %A Persat, Henri %A Mélyne Hautecoeur %A Philippe Keith %K Cytochrome c oxidase subunit 1 %K Esox aquitanicus %K Esox cisalpinus %K France %K Integrative taxonomy %K New species %K Pleiomorphic adenoma gene-like 2 %X

This integrative taxonomy study of French pikes compares morphological characters and molecular sequence data (mitochondrial COI and nuclear Plagl2 genes). In addition to the expected E. lucius, DNA sequences and morphology both support a new species in France, E. aquitanicus sp. nov. from the Charente to the Adour drainages. It is characterized by a color pattern of sides with narrow 1–1.5-scale-wide oblique vertical bands, conferring it a marbled coat, a snout only 0.9 times larger than the postorbital length, an anal fin basis 1.1–1.2 times larger than the caudal peduncle length, 101 to 121 lateral scales, 53 to 57 vertebrae, as well as 24 diagnostic sites in the COI gene and 3 in the Plagl2 gene. Partial COI sequences (131bp) from modern and historical specimens indicate also the presence of E. cisalpinus and E. lucius during the 19th century in Lake Geneva. Morphological and molecular data points to a possible hybridization between E. lucius with both other local pike species, representing a risk for them. Their endangerment status should be evaluated rapidly in order to take conservation measures.

%B Comptes Rendus Biologies %V 337 %P 521-534 %G eng %N 9 %R 10.1016/j.crvi.2014.07.002