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Workshop Cell culture

Primary culture of Abalone hemocytes (24 h)

General topics

This working group aims to share the already acquired knowledge and the development of new methodologies regarding cell culture for their application in the non-conventional aquatic models of the UMR.

The physiological, morphogenetic and developmental investigations undertaken in the UMR are partially based on in vitro approaches as primary culture in teleosts (Anguilla), molluscs (cupped oyster, abalone, cuttlefish), cnidarian and algae.

This working group enables to:

  • Identify and knock down the technical locks (contamination, cell types identification, sustaining of the functional characteristics..),
  • Adapt molecular tools to detect specific markers of our cells of interest,
  • Optimize the culture conditions to obtain stable and perennial cell lines allowing longer-term approaches,
  • Compare the cell responses to environmental stress on our various models.

This transverse theme relies on the technical platforms as the CMAbio (Center of Microscopy applied to the Biology), the flow Cytometry platform of the University of Caen Basse-Normandie, the cell culture labs available on various UMR sites (Paris, marine Station of Concarneau, Caen) and the collection of microalgae (Algobank).

The Research Unit BOREA supported the following incentive projects:

- « Caractérisation moléculaire de cellules souches en culture et tri cellulaire chez deux modèles aquatiques non-conventionnels, la seiche et la petite roussette » (co-resp. Sébastien BaratteAude Andouche, Eq2 Paris, Aude Gautier, Eq2 Caen) : 2015 renouvelé 2016

- « Faisabilité de mesures en impédancemétrie sur des cellules de modèles aquatiques non-conventionnels » (co-resp. Clothilde Heude, Eq2 Caen et Stéphanie Bordenave, Eq4 Concarneau) : 2015