@article {9333, title = {Aluminium-based galvanic anode impacts the photosynthesis of microphytobenthos and supports the bioaccumulation of metals released.}, journal = {Aquat Toxicol}, volume = {258}, year = {2023}, month = {2023 May}, pages = {106501}, abstract = {

Very few studies have looked at the potential biological effects of degradation products of galvanic anodes particularly on primary producers which are central to food webs in marine ecosystems. The galvanic anode cathodic protection system (GACP) is widely used to protect submerged metallic structures from corrosion. Aluminium (Al) and zinc (Zn) are the main constituents of galvanic anodes and are therefore released in the marine environment by oxidation process to form ions or oxy-hydroxides. The main objective of our study was to evaluate the effects of the metals released from an aluminium-based galvanic anode on microphytobenthos performance in term of biofilm growing through the analysis of photosynthetic parameters, the determination of chlorophyll and extracellular polymeric substances (EPS). The bioaccumulation of Al and Zn were measured in the microphytobenthic compartment collected at the surface of polyvinyl chloride (PVC) plates exposed during 13 days to seawaters enriched in different concentrations of metals released from dissolution of one anode. Determination of bioconcentration factors confirmed that the microphytobenthos has incorporated Al. A significative effect was observed on the Chl a concentration for the higher tested concentration ([Al]\ =\ 210.1\ {\textpm}\ 60.2\ {\textmu}g L\ \ ; [Zn]\ =\ 20.2\ {\textpm}\ 1.4\ {\textmu}g L\ \ ). The seawater exposed to the anode affected the MPB productivity (ETRII) with consequences on acclimatation light (Ek), absorption cross section of PSII (σ), F/F and NPQ. Regarding the EPS production, the anode degradation presented an impact on high and low molecular weight of both carbohydrates and protein fractions of microphytobenthos suggesting that EPS play an essential role in sequestering metal contaminants to maintain the integrity of the biological membranes and the functionality of the cellular organelles. The accumulation of Al released by GACP in microphytobenthos cells could lead to physiologic problems in photosynthetic organisms.

}, keywords = {Aluminum, Bioaccumulation, Ecosystem, Electrodes, Photosynthesis, Water Pollutants, Chemical, Zinc}, issn = {1879-1514}, doi = {10.1016/j.aquatox.2023.106501}, author = {Levallois, Alexandre and Vivier, Baptiste and Caplat, Christelle and Goux, Didier and Orvain, Francis and Lebel, Jean-Marc and Claquin, Pascal and Chasselin, L{\'e}o and Basuyaux, Olivier and Serpentini, Antoine} } @article {9329, title = {Comparative effects of trace metal elements released from dissolution of aluminum-based galvanic anodes, aluminum chloride, zinc chloride, and their mixture on the development of the Pacific oyster D-larvae, Crassostrea gigas}, journal = {Environmental Science and Pollution Research}, year = {2023}, month = {Jul-08-2025}, doi = {10.1007/s11356-023-29566-7}, url = {https://link.springer.com/10.1007/s11356-023-29566-7}, author = {Levallois, Alexandre and Katherine Costil and Caplat, Christelle and Basuyaux, Olivier and Lebel, Jean-Marc and Guegan, Claire and Serpentini, Antoine} } @article {9327, title = {Effects on Growth of Juvenile Abalones Haliotis tuberculata Under Chronic Exposition to Metals Released from the Dissolution of an Aluminium-Based Galvanic Anode}, journal = {Archives of Environmental Contamination and Toxicology}, volume = {84}, year = {2023}, month = {Jan-01-2023}, pages = {32 - 44}, abstract = {In the marine environment, the galvanic anode cathodic protection system (GACP) undergoes oxidation and releases metals in the forms of ions or oxy-hydroxides into the environment. The objective of the present study was to investigate the toxicity of a cocktail of metals released from the dissolution of an aluminium-based galvanic anode (~95\% Al,~5\% Zn) on the abalone Haliotis tuberculata. Juveniles were exposed for 16\ weeks (i.e. 12\ weeks of exposure and 4\ weeks of decontamination phase) and their growth, intake rate, conversion rate and metallic concentrations were monitored. A total of 6 conditions were tested: a control, 4 concentrations based on Al and a trophic control. Results showed that the mortality reached 57\% for individuals exposed to 1125\ {\textmu}g L-1 of Al, and the abalone growth signifcantly decreased for an Al concentration greater than 495\ {\textmu}g L-1. At the highest exposure concentration, intake rate measurements revealed that the appetite of abalones was afected, supported by the large increase in the conversion rate which was indicative of a poor feed efciency. The monitoring of metallic concentrations showed that H. tuberculata strongly bioconcentrated Al relative to zinc. The diet did not appear to be the primary pathway for metal entry. Concentrations that signifcantly impacted abalone growth and survival during the experiment were higher than those found in natural environment, but the bioconcentration of Al into the tissues of a primary consumer such as abalone may be a potential pathway for Al to enter food webs.}, issn = {0090-4341}, doi = {10.1007/s00244-022-00975-y}, url = {https://link.springer.com/10.1007/s00244-022-00975-y}, author = {Nivelais, Laureen and Levallois, Alexandre and Basuyaux, Olivier and Katherine Costil and Lebel, Jean-Marc and Larsonneur, Solveig and Guichard, Gwendoline and Serpentini, Antoine and Caplat, Christelle} } @article {9326, title = {Impact assessment of metals realeased by aluminium-based galvanic anode on the physiology of the abalone Haliotis tuberculata in controlled conditions}, journal = {Ecotoxicology}, volume = {32}, year = {2023}, month = {Jan-05-2023}, pages = {438 - 450}, abstract = {To protect metal structures immersed in the sea from corrosion, the galvanic anode cathodic protection system (GACP) is often applied. However, this association leads to continuous oxidation of the galvanic anode and therefore to a release of a metal cocktail in the forms of ions or oxy-hydroxides. Therefore, the main objective of our study was to investigate the toxicity of elements released from the dissolution of an aluminium-based galvanic anode (\~{}95\% Al, \~{}5\% Zn, \<0.1\% for In, Cu, Cd, Mn, Fe) on a grazing gastropod, the abalone Haliotis tuberculata. The present study was carried out in complement to other research currently in submission. Gastropods were exposed for 16 weeks (12 weeks of exposure and 4 weeks of decontamination phase) to 6 conditions including a control, 4 concentrations based on total aluminium level (86, 425, 1096 and 3549 {\textmu}g L-1 ) and a trophic control, corresponding to abalones placed in non-contaminated natural seawater but fed with contaminated algae. The effects of metals on growth, glycogen levels, brix index of hemolymph, MDA levels in digestive gland and gills, hemocyte phagocytic activity, ROS production, lysosomal system and the progress of gametogenesis were investigated throughout the entire exposure allowing the realization of kinetics. The results revealed that the aluminiumbased anode does not seem to have an effect on the health status of the individuals for environmentally realistic concentrations. However, in extreme conditions strong effects were reported on the growth, immune system and reproduction of abalone.}, issn = {0963-9292}, doi = {10.1007/s10646-023-02652-9}, url = {https://link.springer.com/10.1007/s10646-023-02652-9}, author = {Alexandre Levallois and Nivelais, Laureen and Christelle Caplat and Jean-Marc Lebel and Basuyaux, Olivier and Katherine Costil and Antoine Serpentini} } @article {8881, title = {Effects of chronic exposure of metals released from the dissolution of an aluminium galvanic anode on the Pacific oyster Crassostrea gigas}, journal = {Aquatic Toxicology}, volume = {Volume 249}, year = {2022}, abstract = {Among the anthropogenic sources releasing metallic species into the marine environment, the galvanic anode cathodic protection system (GACP) is widely used to protect submerged metallic structures from corrosion. Galvanic anodes are an alloy of metals of which the main component is aluminum or zinc. Very few studies were performed to study their potential biological effects. We investigated the chronic toxicity of an aluminum-based galvanic anode on the Pacific oyster, Crassostrea gigas. Oysters were exposed for 84 days to three concentrations of aluminum (50, 100 and 300 {\textmu}g L-1) obtained with an electrochemical experimental device simulating the dissolution of a galvanic anode. At different exposure times, we studied a battery of biomarkers of the immune system, reproductive parameters and the metabolic state of the oysters. Results demonstrated a sensitivity of oysters at the highest concentration and some biological effects were observed especially for the malondialdehyde content in the digestive gland after 84 days of exposure. In addition to these biomarkers, the bioaccumulation of the different metals composing the anode was measured in oysters{\textquoteright} tissues. Bivalves bioaccumulated more zinc than aluminum, even if aluminium was present in greater concentrations during exposures. Moreover, exposure time did not influence the bioaccumulation of aluminum in contrast to zinc.}, keywords = {aluminium, Bioaccumulation, Biological effects, Crassostrea gigas, galvanic anode, Oyster}, doi = {https://doi.org/10.1016/j.aquatox.2022.106223}, author = {Alexandre Levallois and Christelle Caplat and Olivier Basuyaux and Jean-Marc Lebel and Antoine Laisney and Katherine Costil and Antoine Serpentini} } @article {6631, title = {In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata}, journal = {Fish and Shellfish Immunology}, year = {2020}, abstract = {Glyphosate-based herbicides are among the most produced and widely-used herbicides.\ Studies have shown that commercial formulations and adjuvants may be more toxic to non-target organisms than the active ingredients alone, but the mechanisms of action of these\ chemicals remain unclear. The aim of this study was to investigate the in vitro effects of\ glyphosate, a commercial formulation and adjuvant alone using primary culture of hemocytes\ from the European abalone Haliotis tuberculata, a commonly farmed shellfish. Glyphosate\ was found to have negligible effects on viability, phagocytic activities and lysosome stability\ even with very high doses (i.e. 100 mg L-1). By contrast, greater effects on viability were\ observed for the commercial formulation and adjuvant alone, with EC50 values of 41.42 mg L-1 and 1.85 mg L-1, respectively. These results demonstrate that the toxic sublethal effects (i.e.\ phagocytic activity and destabilization of lysosomal membranes) of formulated glyphosate
came from adjuvants and suggest they may be related to cell and organelle membrane\ destabilization.}, keywords = {glyphosate, Haliotis tuberculata, Hemocytes, neutral red retention assay, Phagocytosis, POEAs, viability assay}, doi = {10.1016/j.fsi.2020.02.058}, url = {https://doi.org/10.1016/j.fsi.2020.02.058}, author = {Mottier, Antoine and Antoine Serpentini and Dallas, Lorna and James, Ad{\`e}le and Lebel, Jean-Marc and Katherine Costil} } @article {8721, title = {In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata}, journal = {Fish \& Shellfish Immunology}, volume = {100}, year = {2020}, month = {Jan-05-2020}, pages = {1 - 8}, issn = {10504648}, doi = {10.1016/j.fsi.2020.02.058}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464820301455}, author = {Mottier, Antoine and Serpentini, Antoine and Dallas, Lorna and James, Ad{\`e}le and Lebel, Jean-Marc and Katherine Costil} } @article {6943, title = {Assessing the impacts of several algae-based diets on cultured European abalone ( Haliotis tuberculata )}, journal = {Aquatic Living Resources}, volume = {31}, year = {2018}, month = {Jan-01-2018}, pages = {28}, doi = {10.1051/alr/2018018}, url = {https://www.alr-journal.org/10.1051/alr/2018018}, author = {Basuyaux, Olivier and Blin, Jean-Louis and Katherine Costil and Richard, Olivier and Lebel, Jean-Marc and Antoine Serpentini} } @article {6940, title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes- Application for Cartilage Engineering.}, journal = {Mar Biotechnol (NY)}, volume = {20}, year = {2018}, month = {2018 Aug}, pages = {436-450}, abstract = {

The shells of the bivalve mollusks are organo-mineral structures predominantly composed of calcium carbonate, but also of a minor organic matrix, a mixture of proteins, glycoproteins, and polysaccharides. These proteins are involved in mineral deposition and, more generally, in the spatial organization of the shell crystallites in well-defined microstructures. In this work, we extracted different organic shell extracts (acid-soluble matrix, acid-insoluble matrix, water-soluble matrix, guanidine HCl/EDTA-extracted matrix, referred as ASM, AIM, WSM, and EDTAM, respectively) from the shell of the scallop Pecten maximus and studied their biological activities on human articular chondrocytes (HACs). We found that these extracts differentially modulate the biological activities of HACs, depending on the type of extraction and the concentration used. Furthermore, we showed that, unlike ASM and AIM, WSM promotes maintenance of the chondrocyte phenotype in monolayer culture. WSM increased the expression of chondrocyte-specific markers (aggrecan and type II collagen), without enhancing that of the main chondrocyte dedifferentiation marker (type I collagen). We also demonstrated that WSM could favor redifferentiation of chondrocyte in collagen sponge scaffold in hypoxia. Thus, this study suggests that the organic matrix of Pecten maximus, particularly WSM, may contain interesting molecules with chondrogenic effects. Our research emphasizes the potential use of WSM of Pecten maximus for cell therapy of cartilage.

}, keywords = {Aged, Aged, 80 and over, Aggrecans, Animal Shells, Animals, Cell Differentiation, Cells, Cultured, Chondrocytes, Collagen Type II, Extracellular Matrix, Gene Expression Profiling, Humans, Middle Aged, Pecten, Phenotype}, issn = {1436-2236}, doi = {10.1007/s10126-018-9807-7}, author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Antoine Serpentini and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence} } @article {8720, title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes{\textemdash} Application for Cartilage Engineering}, journal = {Marine Biotechnology}, volume = {20}, year = {2018}, month = {Jan-08-2018}, pages = {436 - 450}, issn = {1436-2228}, doi = {10.1007/s10126-018-9807-7}, url = {http://link.springer.com/10.1007/s10126-018-9807-7}, author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Serpentini, Antoine and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence} } @article {8718, title = {Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure}, journal = {Cytotechnology}, volume = {69}, year = {2017}, month = {Jan-04-2017}, pages = {191 - 200}, issn = {0920-9069}, doi = {10.1007/s10616-016-0050-7}, url = {http://link.springer.com/10.1007/s10616-016-0050-7}, author = {Ladhar-Chaabouni, Rim and Houel, Thomas and Serpentini, Antoine and Karray, Sahar and Lebel, Jean-Marc and Hamza-Chaffai, Amel} } @article {8719, title = {Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro}, journal = {Cytotechnology}, volume = {69}, year = {2017}, month = {Jan-10-2017}, pages = {815 - 829}, issn = {0920-9069}, doi = {10.1007/s10616-017-0096-1}, url = {http://link.springer.com/10.1007/s10616-017-0096-1}, author = {Latire, Thomas and Legendre, Florence and Bouyoucef, Mouloud and Marin, Fr{\'e}d{\'e}ric and Carreiras, Franck and Rigot-Jolivet, Muriel and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Serpentini, Antoine} } @article {4797, title = {Sub-lethal effects of a glyphosate-based commercial formulation and adjuvants on juvenile oysters (Crassostrea gigas) exposed for 35 days}, journal = {Marine Pollution Bulletin}, year = {2017}, keywords = {Crassostrea gigas, individual biomarkers, mortality, oxidative stress, POEAs, Roundup}, author = {S{\'e}guin, Alexis and Mottier, Antoine and Perron, Carole and Lebel, Jean-Marc and Antoine Serpentini and Katherine Costil} } @article {3506, title = {Sub-chronic exposure to fluoxetine in juvenile oysters (Crassostrea gigas): uptake and biological effects.}, journal = {Environ Sci Pollut Res Int}, year = {2016}, month = {2014 Oct 15}, abstract = {

The bioconcentration potential of fluoxetine (FLX) and its biological effects were investigated in juvenile Pacific oyster exposed for 28\ days to environmentally relevant concentrations of FLX (1\ ng L(-1), 100\ ng L(-1) and up to 10\ μg\ L(-1)). FLX bioaccumulated in oyster flesh resulting in 28-day bioconcentration factors greater than 2,000 and 10,000 by referring to wet and dry weights, respectively. Nevertheless, FLX did not induce oyster mortality, delayed gametogenesis, or lead to adverse histopathological alterations. At the two highest concentrations, despite non-optimal trophic conditions, FLX stimulated shell growth but only in a transient manner, suggesting a role of serotonin in the regulation of feeding and metabolism in bivalves. Those high concentrations seemed to drive bell-shaped responses of catalase and glutathione S-transferase activities throughout the exposure period, which may indicate the activation of antioxidant enzyme synthesis and then an enhanced catabolic rate or direct inhibition of those enzymes. However, no clear oxidative stress was detected because no strong differences in thiobarbituric acid-reactive substance (TBARS) content (i.e. lipid peroxidation) were observed between oyster groups, suggesting that cellular defence mechanisms were effective. These results demonstrate the importance of considering additional biomarkers of oxidative stress to obtain a comprehensive overview of the FLX-induced changes in marine bivalves exposed under realistic conditions. Considering the battery of biomarkers used, FLX appears to induce little or no effects on oyster physiology even at a concentration of 10\ μg\ L(-1). These results do not confirm the lowest observed effect concentration (LOEC) values reported by some authors in other mollusc species.

}, issn = {1614-7499}, doi = {10.1007/s11356-014-3702-1}, author = {Di Poi, C and L Evariste and S{\'e}guin, Alexis and Mottier, A and Pedelucq, Julie and Jean-Marc Lebel and Antoine Serpentini and Budzinski, H{\'e}l{\`e}ne and Katherine Costil} } @article {3507, title = {Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata?}, journal = {Environ Sci Pollut Res Int}, year = {2015}, month = {2014 Aug 19}, abstract = {

In this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24\ h to CdCl2 concentrations of 0, 1 and 100\ μg\ ml(-1). The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1\ μg\ ml(-1) of CdCl2.

}, issn = {1614-7499}, doi = {10.1007/s11356-014-3387-5}, author = {Ladhar-Chaabouni, R and Machreki-Ajmi, Monia and Antoine Serpentini and Jean-Marc Lebel and Hamza-Chaffai, A} } @article {3505, title = {Effects of subchronic exposure to glyphosate in juvenile oysters (Crassostrea gigas): From molecular to individual levels.}, journal = {Mar Pollut Bull}, year = {2015}, month = {2014 Oct 30}, abstract = {

Glyphosate-based herbicides are extensively used and can be measured in aquatic ecosystems, including coastal waters. The effect of glyphosate on non-target organisms is an issue of worldwide concern. The aim of this study was to investigate the effects of subchronic exposure to glyphosate in juvenile oysters, Crassostrea gigas. Yearling oysters were exposed to three concentrations of glyphosate (0.1, 1 and 100μgL(-1)) for 56days. Various endpoints were studied, from the individual level (e.g., gametogenesis and tissue alterations) to the molecular level (mRNA quantification), including biochemical endpoints such as glutathione-S-transferase (GST) and catalase activities and malondialdehyde content. No mortality and growth occurred during the experiment, and individual biomarkers revealed only slight effects. The levels of gene expression significantly increased in oysters exposed to the highest glyphosate concentration (GST and metallothioneins) or to all concentrations (multi-xenobiotic resistance). These results suggested an activation of defence mechanisms at the molecular level.

}, issn = {1879-3363}, doi = {10.1016/j.marpolbul.2014.10.026}, author = {Mottier, A and S{\'e}guin, Alexis and Devos, Alexandre and Le Pabic, C and Voiseux, C and Lebel, Jean-Marc and Antoine Serpentini and Fievet, B and Katherine Costil} } @article {3510, title = {Acute toxicity of 8 antidepressants: what are their modes of action?}, journal = {Chemosphere}, volume = {108}, year = {2014}, month = {2014 Aug}, pages = {314-9}, abstract = {

Currently, the hazard posed by pharmaceutical residues is a major concern of ecotoxicology. Most of the antidepressants belong to a family named the Cationic Amphipathic Drugs known to have specific interactions with cell membranes. The present study assessed the impact of eight antidepressants belonging to selective serotonin reuptake inhibitors or serotonin norepinephrine reuptake inhibitors by the combination of multi-approaches (in vivo, in vitro, in silico) and gives some insights on the mode of action for these molecules. Antidepressants were from the most to the least toxic compound for Daphnia magna: Sertraline (EC50=1.15 mg L(-1))\>Clomipramine (2.74 mg L(-1))\>Amitriptyline (4.82 mg L(-1))\>Fluoxetine (5.91 mg L(-1))\>Paroxetine (6.24 mg L(-1))\>Mianserine (7.81 mg L(-1))\>Citalopram (30.14 mg L(-1)) and Venlafaxine (141.28 mg L(-1)). These acute toxicities were found correlated to Log Kow coefficients (R=0.93, p\<0.001) and to cytotoxicity assessed on abalone hemocytes through the neutral red uptake assay (R=0.96, p\<0.001). If narcosis as mode of action is typically expected during acute ecotoxicity bioassays, we showed by molecular modeling that particular interactions can exist between antidepressants and phosphatidylcholine, a major component of cell membranes, leading to a more specific mode of action corresponding to a potential acidic hydrolysis of ester functions.

}, keywords = {Animals, Antidepressive Agents, Cell Survival, Cells, Cultured, Daphnia, Environmental Pollutants, Gastropoda, Hemocytes, Lysosomes, Models, Molecular, Phosphatidylcholines, Serotonin Uptake Inhibitors}, issn = {1879-1298}, doi = {10.1016/j.chemosphere.2014.01.057}, author = {Minguez, Laetitia and Farcy, E and Ballandonne, C{\'e}line and Lepailleur, Alban and Antoine Serpentini and Jean-Marc Lebel and Bureau, Ronan and Halm-Lemeille, Marie-Pierre} } @article {3512, title = {Assessment of cytotoxic and immunomodulatory properties of four antidepressants on primary cultures of abalone hemocytes (Haliotis tuberculata).}, journal = {Aquat Toxicol}, volume = {153}, year = {2014}, month = {2014 Aug}, pages = {3-11}, abstract = {

Pharmaceutical compounds like antidepressants found in surface waters raise concerns due to their potential toxicity on non-target aquatic organisms. This study aimed at investigating the in vitro cytotoxicity and immunomodulatory properties of four common antidepressants, namely Amitriptyline, Clomipramine, Citalopram and Paroxetine, on primary cultures of abalone hemocytes (Haliotis tuberculata), after 48 h-exposure. Effects on immunocompetence (phagocytosis, levels of reactive oxygen species, esterase activity and lysosomal membrane destabilization) were assessed. Results obtained by MTT assays revealed that acute toxicity is unlikely to occur in the environment since the LC50s of the four antidepressants are at the mg/L level. The different immunological endpoints displayed a biphasic response, with an increase at the lowest concentration (i.e. 1 μg/L) followed by a decrease at higher concentrations. Overall, Amitriptyline and Clomipramine, the two tricyclic antidepressants, had higher immunomodulatory capacities than the two selective serotonin reuptake inhibitors Citalopram and Paroxetine. Amitriptyline was the most potent and Citalopram the least potent drug in altering immune function in H. tuberculata.

}, keywords = {Animals, Antidepressive Agents, Cell Survival, Cells, Cultured, Enzyme Activation, Esterases, Gastropoda, Hemocytes, Immunity, Innate, Lethal Dose 50, Phagocytosis, Reactive Oxygen Species, Water Pollutants, Chemical}, issn = {1879-1514}, doi = {10.1016/j.aquatox.2013.10.020}, author = {Minguez, Laetitia and Halm-Lemeille, Marie-Pierre and Katherine Costil and Bureau, Ronan and Jean-Marc Lebel and Antoine Serpentini} } @article {3428, title = {Comparison of the sensitivity of seven marine and freshwater bioassays as regards antidepressant toxicity assessment.}, journal = {Ecotoxicology}, volume = {23}, year = {2014}, month = {2014 Nov}, pages = {1744-54}, abstract = {

The hazards linked to pharmaceutical residues like antidepressants are currently a major concern of ecotoxicology because they may have adverse effects on non-target aquatic organisms. Our study assesses the ecotoxicity of three antidepressants (fluoxetine, sertraline and clomipramine) using a battery of marine and freshwater species representing different trophic levels, and compares the bioassay sensitivity levels. We selected the following bioassays: the algal growth inhibition test (Skeletonema marinoi and Pseudokirchneriella subcapitata), the microcrustacean immobilization test (Artemia salina and Daphnia magna), development and adult survival tests on Hydra attenuata, embryotoxicity and metamorphosis tests on Crassostrea gigas, and in vitro assays on primary cultures of Haliotis tuberculata hemocytes. The results showed high inter-species variability in EC50-values ranging from 43 to 15,600\ {\textmu}g/L for fluoxetine, from 67 to 4,400\ {\textmu}g/L for sertraline, and from 4.70\ {\textmu}g/L to more than 100,000\ {\textmu}g/L for clomipramine. Algae (S. marinoi and P. subcapitata) and the embryo-larval stages of the oyster C. gigas were the most sensitive taxa. This raises an issue due to their ecological and/or economic importance. The marine crustacean A. salina was the least sensitive species. This difference in sensitivity between bioassays highlights the importance of using a test battery.

}, issn = {1573-3017}, doi = {10.1007/s10646-014-1339-y}, author = {Minguez, Laetitia and Di Poi, C and Farcy, E and Ballandonne, C{\'e}line and Benchouala, Amira and Bojic, Cl{\'e}ment and Cossu-Leguille, Carole and Katherine Costil and Antoine Serpentini and Jean-Marc Lebel and Halm-Lemeille, Marie-Pierre} } @article {3509, title = {The effect of different polychlorinated biphenyls on two aquatic models, the green alga Pseudokirchneriella subcapitata and the haemocytes from the European abalone Haliotis tuberculata.}, journal = {Chemosphere}, volume = {110}, year = {2014}, month = {2014 Sep}, pages = {120-8}, abstract = {

The present study was conducted to determine the toxicity of different polychlorinated biphenyls (PCBs) on the green algae, Pseudokirchneriella subcapitata and the haemocytes from the European abalone, Haliotis tuberculata. Using the algal growth inhibition test, the green algae median Effective Concentration (EC50) values ranged from 0.34μM for PCB28 to more than 100μM for PCBs 101 and 153. Considering the MTT viability test, the abalone EC50 values ranged from 1.67μM for PCB153 to 89μM for PCB28. Our results in contrast to previous observation in vertebrates did not show significant differences between the dioxin like- and non dioxin like-PCBs toxicities regardless of the model used. However, our results demonstrated that the toxicities of PCBs were species dependent. For example, PCB28 was the most toxic compound for P. subcapitata whereas PCBs 1, 180 and 153 were less toxic for that species. On the contrary, PCB153 was reported as the most toxic for H. tuberculata haemocytes and PCB28 the least toxic. To investigate the mode of action of these compounds, we used an in silico method. Our results suggested that PCBs have a non-specific mode of action (e.g., narcosis) on green algae, and another mode of action, probably more specific than narcosis, was reported for PCBs on the abalone haemocytes.

}, keywords = {Animals, Chlorophyta, Environmental Pollutants, Gastropoda, Hemocytes, Polychlorinated Biphenyls, Tetrachlorodibenzodioxin}, issn = {1879-1298}, doi = {10.1016/j.chemosphere.2014.02.023}, author = {Halm-Lemeille, Marie-Pierre and Abbaszadeh Fard, Elham and Latire, T and Ferard, Jean-Fran{\c c}ois and Katherine Costil and Jean-Marc Lebel and Bureau, Ronan and Antoine Serpentini} } @article {3511, title = {Effects of acute exposures to mecoprop, mecoprop-p and their biodegradation product (2-MCP) on the larval stages of the Pacific oyster, Crassostrea gigas.}, journal = {Aquat Toxicol}, volume = {146}, year = {2014}, month = {2014 Jan}, pages = {165-75}, abstract = {

Studies have shown that pesticides are sometimes detected at rather high levels in seawater and it has been suggested that these chemical compounds could act as additional stress factor for oysters cultured in coastal environments. The effects of pesticides on marine molluscs could be particularly harmful in the early stages which correspond to critical life stages. This study aimed to assess the effects of mecoprop, mecoprop-p and their degradation compound 2-methyl-4-chlorophenol on two larval stages of Crassostrea gigas. Embryotoxic effects were assessed on veliger larvae after 36 h exposures, and both percentages of normal larvae and types of abnormalities were taken into account. The effects of the three substances were evaluated on 21-day-old pediveliger larvae by calculating metamorphosis rates after 24h exposures. The results of the embryotoxicity assay indicated that 2-methyl-4-chlorophenol was more toxic (EC50: 10.81 mg L(-1)) than its parent compounds (EC50 mecoprop: 42.55 mg L(-1); EC50 mecoprop-p: 78.85 mg L(-1)). Mecoprop in particular injured shell formation with an increase of shell abnormalities following herbicide concentrations. The active substances were not toxic to metamorphosis processes, but 2-MCP was revealed to be more toxic to the success of metamorphosis (EC50: 7.20 mg L(-1)) than to embryo-larval development. However, the toxic concentrations were several orders of magnitude higher than environmental concentrations.

}, keywords = {2-Methyl-4-chlorophenoxyacetic Acid, Animals, Chlorophenols, Crassostrea, Embryo, Nonmammalian, Larva, Metamorphosis, Biological, Water Pollutants, Chemical}, issn = {1879-1514}, doi = {10.1016/j.aquatox.2013.11.008}, author = {Mottier, A and Kientz-Bouchart, Val{\'e}rie and Dubreule, Christelle and Antoine Serpentini and Jean-Marc Lebel and Katherine Costil} } @article {3508, title = {Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).}, journal = {Fish Shellfish Immunol}, volume = {40}, year = {2014}, month = {2014 Oct}, pages = {362-73}, abstract = {

Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.

}, issn = {1095-9947}, doi = {10.1016/j.fsi.2014.07.020}, author = {Le Pabic, C and Goux, Didier and Guillamin, Maryline and Safi, Georges and Jean-Marc Lebel and Sim{\'e}oni Koueta-Noussith{\'e} and Antoine Serpentini} } @article {4634, title = {In vitro effect of five pharmaceuticals on the viability of the European abalone hemocytes, Haliotis tuberculata}, journal = {J xenobiotics}, volume = {4}, year = {2014}, pages = {78-80}, keywords = {cell viability, Haliotis tuberculata, hemocyte, in vitro, pharmaceuticals}, doi = {10.4081/xeno.2014.4900}, author = {Letullier, Amandine and Minguez, Laetitia and Katherine Costil and Halm-Lemeille, Marie-Pierre and Jean-Marc Lebel and Antoine Serpentini} } @article {3306, title = {Prophenoloxidase system, lysozyme and protease inhibitor distribution in the common cuttlefish Sepia officinalis}, year = {2014}, author = {Le Pabic, C and Safi, Georges and Antoine Serpentini and Jean-Marc Lebel and Jean-Paul Robin and Sim{\'e}oni Koueta-Noussith{\'e}} } @article {3427, title = {Shell extracts from the marine bivalve Pecten maximus regulate the synthesis of extracellular matrix in primary cultured human skin fibroblasts.}, journal = {PLoS One}, volume = {9}, year = {2014}, month = {2014}, pages = {e99931}, abstract = {

Mollusc shells are composed of more than 95\% calcium carbonate and less than 5\% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the -112/-61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.

}, issn = {1932-6203}, doi = {10.1371/journal.pone.0099931}, author = {Latire, T and Legendre, Florence and Bigot, Nicolas and Carduner, Ludovic and Kellouche, Sabrina and Bouyoucef, Mouloud and Carreiras, Franck and Marin, F and Jean-Marc Lebel and Gal{\'e}ra, Philippe and Antoine Serpentini} }