@article {7539, title = {Exploring brain diversity in crustaceans: sensory systems of deep vent shrimpsAbstract}, journal = {Neuroforum}, year = {2020}, month = {Nov-04-2021}, issn = {0947-0875}, doi = {10.1515/nf-2020-0009}, url = {https://www.degruyter.com/view/journals/nf/ahead-of-print/article-10.1515-nf-2020-0009/article-10.1515-nf-2020-0009.xml}, author = {Machon, Julia and Krieger, Jakob and Magali Zbinden and Juliette Ravaux and Harzsch, Steffen} } @article {7540, title = {Rimicaris exoculata: biology and ecology of a shrimp from deep-sea hydrothermal vents associated with ectosymbiotic bacteria}, journal = {Marine Ecology Progress Series}, volume = {652}, year = {2020}, month = {Mar-10-2021}, pages = {187 - 222}, abstract = {Rimicaris exoculata, the {\textquoteleft}blind shrimp,{\textquoteright} is the most abundant species living on active hydrothermal edifices at deep-sea vents of the Mid-Atlantic Ridge. Its unusually enlarged branchial chamber houses a dense ectosymbiotic community of chemoautotrophic bacteria. Long debated, shrimp nutrition has been proven to be a kind of osmotrophy, whereby small organic molecules produced by the symbionts pass through the integument of the shrimp directly into the circulatory system, rather than through the digestive system. The broad phylogenetic and metabolic diversity of this epibiotic community suggests a highly flexible and adjustable microbial consortium, adapted to the chemically contrasting environments inhabited by the shrimp. To cope with the highly fluctuating oxygen and temperature conditions of its habitat, R. exoculata possesses hemocyanin with a strong oxygen affinity, and has developed both molecular and behavioral responses to heat stresses. If R. exoculata is able to detect very dim light or chemical compounds emitted by vents, the relatively small visual and olfactory areas in the brain, along with the disproportionately enlarged higher centers, argue for a significant involvement of navigational skills using learning and place memory to orient itself within its aphotic environment. This shrimp undergoes unconventional larval development, with a primary lecithotrophic stage followed by an extended planktotrophic period, allowing a huge potential for dispersion. In light of mining licenses posing a threat for deep-sea environments, this species is a model still to be studied to better understand life in extreme deep-sea ecosystems at the global scale of an ocean.}, issn = {0171-8630}, doi = {10.3354/meps13467}, url = {https://www.int-res.com/abstracts/meps/v652/p187-222/}, author = {Magali Zbinden and Cambon-Bonavita, MA} } @article {6816, title = {Neuroanatomy of a hydrothermal vent shrimp provides insights into the evolution of crustacean integrative brain centers}, journal = {eLife}, volume = {8}, year = {2019}, month = {Jun-08-2019}, abstract = {Alvinocaridid shrimps are emblematic representatives of the deep hydrothermal vent
fauna at the Mid-Atlantic Ridge. They are adapted to a mostly aphotic habitat with extreme
physicochemical conditions in the vicinity of the hydrothermal fluid emissions. Here, we
investigated the brain architecture of the vent shrimp Rimicaris exoculata to understand possible
adaptations of its nervous system to the hydrothermal sensory landscape. Its brain is modified from
the crustacean brain ground pattern by featuring relatively small visual and olfactory neuropils that
contrast with well-developed higher integrative centers, the hemiellipsoid bodies. We propose that
these structures in vent shrimps may fulfill functions in addition to higher order sensory processing
and suggest a role in place memory. Our study promotes vent shrimps as fascinating models to
gain insights into sensory adaptations to peculiar environmental conditions, and the evolutionary
transformation of specific brain areas in Crustacea.}, doi = {10.7554/eLife.47550.001}, url = {https://elifesciences.org/articles/47550}, author = {Machon, Julia and Krieger, Jakob and Meth, Rebecca and Magali Zbinden and Juliette Ravaux and Montagn{\'e}, Nicolas and Chertemps, Thomas and Harzsch, Steffen} } @article {6818, title = {Blow Your Nose, Shrimp! Unexpectedly Dense Bacterial Communities Occur on the Antennae and Antennules of Hydrothermal Vent Shrimp}, journal = {Frontiers in Marine Science}, volume = {5}, year = {2018}, month = {Aug-10-2018}, abstract = {In crustaceans, as in other animals, perception of environmental cues is of key importance for a wide range of interactions with the environment and congeners. Chemoreception involves mainly the antennae and antennules, which carry sensilla that detect water-borne chemicals. The functional importance of these as exchange surfaces in the shrimp{\textquoteright}s sensory perception requires them to remain free of any microorganism and deposit that could impair the fixation of odorant molecules on sensory neurons. We report here the occurrence of an unexpected dense bacterial colonization on surface of the antennae and antennules of four hydrothermal vent shrimp species. Microscopic observation, qPCR and 16S rRNA barcoding reveal the abundance, diversity and taxonomic composition of these bacterial communities, that are compared with those found on a related coastal shrimp. Bacterial abundances vary among species. Bacteria are almost absent in coastal shrimp, meanwhile they fully cover the antennal flagella in some hydrothermal vent species. Epsilon- and Gammaproteobacteria dominate the hydrothermal shrimp-associated communities, whereas Alphaproteobacteria and Bacteroidetes are dominant in the coastal ones. Bacteria associated with vent shrimp species are most similar to known chemoautotrophic sulfur-oxidizers. Potential roles of these bacteria on the hydrothermal shrimp antennae and antennules and on sensory functions are discussed.}, doi = {10.3389/fmars.2018.00357}, url = {https://www.frontiersin.org/article/10.3389/fmars.2018.00357/full}, author = {Magali Zbinden and Gallet, Alison and Szafranski, Kamil M. and Machon, Julia and Juliette Ravaux and L{\'e}ger, Nelly and Duperron, S{\'e}bastien} } @article {6819, title = {Comparison of Chemoreceptive Abilities of the Hydrothermal Shrimp Mirocaris fortunata and the Coastal Shrimp Palaemon elegans}, journal = {Chemical Senses}, volume = {43}, year = {2018}, month = {06}, pages = {489-501}, abstract = {Chemoreception might play an important role for endemic shrimp that inhabit deep and dark hydrothermal vents to find food sources and to locate active edifices that release specific chemicals. We compared the chemosensory abilities of the hydrothermal shrimp Mirocaris fortunata and the coastal related species, Palaemon elegans. The detection of diverse ecologically relevant chemical stimuli by the antennal appendages was measured with electroantennography. The 2 species can detect food-related odor and sulfide, a short-distance stimulus, via both their antennae and antennules. Neither iron nor manganese, considered as long-distance stimuli, was detected by the antennal appendages. Investigation of the ultrastructure of aesthetasc sensilla revealed no specific features of the hydrothermal species regarding innervation by olfactory sensory neurons. Pore-like structures occurring in the aesthetasc cuticle and dense bacterial covering seem to be unique to hydrothermal species, but their potential link to chemoreception remains elusive.}, issn = {0379-864X}, doi = {10.1093/chemse/bjy041}, url = {https://doi.org/10.1093/chemse/bjy041}, author = {Machon, Julia and Lucas, Philippe and Juliette Ravaux and Magali Zbinden} } @article {6817, title = {Gill chamber and gut microbial communities of the hydrothermal shrimp Rimicaris chacei Williams and Rona 1986: A possible symbiosis}, journal = {PLOS ONE}, volume = {13}, year = {2018}, month = {Feb-11-2018}, pages = {e0206084}, abstract = {
Rimicaris chacei Williams and Rona 1986, formerly named as Chorocaris chacei, is a caridean
shrimp living in deep-sea hydrothermal ecosystems. This shrimp is endemic to the Mid
Atlantic Ridge (MAR) and lives at the periphery of aggregates of its well-known congeneric
R. exoculata Williams and Rona 1986. Contrasting with the very dense and mobile clusters
formed by R. exoculata, R. chacei lives in small groups of several individuals that are not
very mobile. Although devoid of the characteristic hypertrophied cephalothorax of R. exoculata,
which harbors the ectosymbionts, a microbial community has also been reported in the
cephalothorax of R. chacei. Previous data on morphology, behavior and isotopic values indicate
a diet based on a combination of feeding on its epibiotic bacteria and scavenging or
occasional predation. In this study, our objective was to describe, for the first time, the distribution,
morphology and phylogeny of the microbial communities associated with R. chacei.
This species is significantly less studied than R. exoculata, but nevertheless represents the
only other known example of symbiosis in crustaceans of MAR hydrothermal vent sites.
Microbial communities have been observed at the same locations as in R. exoculata
(mouthparts, branchiostegites and digestive tract). However, in R. chacei, the surfaces
occupied by the bacteria are smaller. The main lineages are affiliated to Epsilon and Gammaproteobacteria
in the cephalothorax and to Deferribacteres, Mollicutes, Epsilon and
Gammaproteobacteria in the digestive tract. Comparison with the well-described bacterial
communities of R. exoculata and hypotheses about the role of these communities in R. chacei
are discussed.
}, doi = {10.1371/journal.pone.0206084}, url = {http://dx.plos.org/10.1371/journal.pone.0206084}, author = {Apremont, Vincent and Cambon-Bonavita, Marie-Anne and Cueff-Gauchard, Val{\'e}rie and Fran{\c c}ois, David and Pradillon, Florence and Laure Corbari and Magali Zbinden}, editor = {Kuo, Chih-Horng} } @article {4931, title = {Comparative Study of Chemosensory Organs of Shrimp From Hydrothermal Vent and Coastal Environments}, journal = {Chemical Senses}, volume = {doi:10.1093/chemse/bjx007}, year = {2017}, pages = {1-13}, abstract = {

The detection of chemical signals is involved in a variety of crustacean behaviors, such as social
interactions, search and evaluation of food and navigation in the environment. At hydrothermal
vents, endemic shrimp may use the chemical signature of vent fluids to locate active edifices,
however little is known on their sensory perception in these remote deep-sea habitats. Here,
we present the first comparative description of the sensilla on the antennules and antennae
of 4 hydrothermal vent shrimp (Rimicaris exoculata, Mirocaris fortunata, Chorocaris chacei,
and Alvinocaris markensis) and of a closely related coastal shrimp (Palaemon elegans). These
observations revealed no specific adaptation regarding the size or number of aesthetascs
(specialized unimodal olfactory sensilla) between hydrothermal and coastal species. We also
identified partial sequences of the ionotropic receptor IR25a, a co-receptor putatively involved in
olfaction, in 3 coastal and 4 hydrothermal shrimp species, and showed that it is mainly expressed
in the lateral flagella of the antennules that bear the unimodal chemosensilla aesthetascs.

}, keywords = {aesthetascs, decapod, hydrothermal shrimp, IR25a, olfaction}, author = {Magali Zbinden and Berthod, C and Montagn{\'e}, N and Machon, J and L{\'e}ger, N and Chertemps, T and Rabet, N and Bruce Shillito and Juliette Ravaux} } @article {6820, title = {Identifying Toxic Impacts of Metals Potentially Released during Deep-Sea Mining{\textemdash}A Synthesis of the Challenges to Quantifying Risk}, journal = {Frontiers in Marine Science}, volume = {4}, year = {2017}, pages = {368}, abstract = {In January 2017, the International Seabed Authority released a discussion paper on the development of Environmental Regulations for deep-sea mining (DSM) within the Area Beyond National Jurisdiction (the {\textquotedblleft}Area{\textquotedblright}). With the release of this paper, the prospect for commercial mining in the Area within the next decade has become very real. Moreover, within nations{\textquoteright} Exclusive Economic Zones, the exploitation of deep-sea mineral ore resources could take place on very much shorter time scales and, indeed, may have already started. However, potentially toxic metal mixtures may be released at sea during different stages of the mining process and in different physical phases (dissolved or particulate). As toxicants, metals can disrupt organism physiology and performance, and therefore may impact whole populations, leading to ecosystem scale effects. A challenge to the prediction of toxicity is that deep-sea ore deposits include complex mixtures of minerals, including potentially toxic metals such as copper, cadmium, zinc, and lead, as well as rare earth elements. Whereas the individual toxicity of some of these dissolved metals has been established in laboratory studies, the complex and variable mineral composition of seabed resources makes the a priori prediction of the toxic risk of DSM extremely challenging. Furthermore, although extensive data quantify the toxicity of metals in solution in shallow-water organisms, these may not be representative of the toxicity in deep-sea organisms, which may differ biochemically and physiologically and which will experience those toxicants under conditions of low temperature, high hydrostatic pressure, and potentially altered pH. In this synthesis, we present a summation of recent advances in our understanding of the potential toxic impacts of metal exposure to deep-sea meio- to megafauna at low temperature and high pressure, and consider the limitation of deriving lethal limits based on the paradigm of exposure to single metals in solution. We consider the potential for long-term and far-field impacts to key benthic invertebrates, including the very real prospect of sub-lethal impacts and behavioral perturbation of exposed species. In conclusion, we advocate the adoption of an existing practical framework for characterizing bulk resource toxicity in advance of exploitation.}, issn = {2296-7745}, doi = {10.3389/fmars.2017.00368}, url = {https://www.frontiersin.org/article/10.3389/fmars.2017.00368}, author = {Hauton, Chris and Brown, Alastair and Thatje, Sven and Mestre, N{\'e}lia C. and Bebianno, Maria J. and Martins, In{\^e}s and Bettencourt, Raul and Canals, Miquel and Sanchez-Vidal, Anna and Bruce Shillito and Juliette Ravaux and Magali Zbinden and Duperron, S{\'e}bastien and Mevenkamp, Lisa and Vanreusel, Ann and Gambi, Cristina and Dell{\textquoteright}Anno, Antonio and Danovaro, Roberto and Gunn, Vikki and Weaver, Phil} } @article {5740, title = {Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata}, journal = {Aquatic Biology}, volume = {175}, year = {2016}, pages = {277-285}, doi = {https://doi.org/10.1016/j.aquatox.2016.03.024}, author = {Auguste, M and Mestre, N and Rocha, T and Cardoso, C and Cueff-Gauchard, V and Le Bloa, S and Cambon-Bonavita, M-A and Bruce Shillito and Magali Zbinden and Juliette Ravaux and Bebianno, M} } @article {6821, title = {New electroantennography method on a marine shrimp in water}, journal = {Journal of Experimental Biology}, volume = {219}, year = {2016}, pages = {3696{\textendash}3700}, abstract = {Antennular chemoreception in aquatic decapods is well studied via the recording of single chemoreceptor neuron activity in the antennule, but global responses of the antennule (or antennae in insects) by electroantennography (EAG) has so far been mainly restricted to aerial conditions. We present here a well-established underwater EAG method to record the global antennule activity in the marine shrimp Palaemon elegans in natural (aqueous) conditions. EAG responses to food extracts, recorded as net positive deviations of the baseline, are reproducible, dose-dependent and exhibit sensory adaptation. This new EAG method opens a large field of possibilities for studying in vivo antennular chemoreception in aquatic decapods, in a global approach to supplement current, more specific techniques.}, issn = {0022-0949}, doi = {10.1242/jeb.140947}, url = {https://jeb.biologists.org/content/219/23/3696}, author = {Machon, Julia and Juliette Ravaux and Magali Zbinden and Lucas, Philippe} } @article {6823, title = {Plasticity and acquisition of the thermal tolerance (upper thermal limit and heat shock response) in the intertidal species Palaemon elegans}, journal = {Journal of Experimental Marine Biology and Ecology}, volume = {484}, year = {2016}, pages = {39 - 45}, abstract = {The marine species sensitivity to climate change will depend on the ways by which these species can adapt to thermal increase and heterogeneity. Here, we present evidence that the intertidal shrimp Palaemon elegans acclimates its thermal tolerance, in response to environmental water temperature, through a significant shift of its upper thermal limit with no concomittant acclimation of the heat shock response (hsp70 stress gene expression threshold). This species is less thermotolerant than its congener Palaemonetes varians, and would therefore potentially be more sensitive to an increase in environmental temperature, such as imposed by global warming. In P. elegans life cycle, physiological adjustments like the shift of the thermal limit and the acquisition of a significant HSR, occurred during the metamorphosis from larvae to post-larvae. This suggests that this step is a genetically-programmed milestone in the process of thermal tolerance acquisition.}, keywords = {acclimation, Caridea, Development, heat stress, hsp70, thermal biology}, issn = {0022-0981}, doi = {https://doi.org/10.1016/j.jembe.2016.07.003}, url = {http://www.sciencedirect.com/science/article/pii/S0022098116301125}, author = {Juliette Ravaux and L{\'e}ger, Nelly and Rabet, Nicolas and Fourgous, Claire and Voland, Guillaume and Magali Zbinden and Bruce Shillito} } @article {6824, title = {Behavioural study of two hydrothermal crustacean decapods: Mirocaris fortunata and Segonzacia mesatlantica, from the Lucky Strike vent field (Mid-Atlantic Ridge)}, journal = {Deep Sea Research Part II: Topical Studies in Oceanography}, volume = {121}, year = {2015}, pages = {146 - 158}, abstract = {Identifying the factors driving community dynamics in hydrothermal vent communities, and in particular biological interactions, is challenged by our ability to make direct observations and the difficulty to conduct experiments in those remote ecosystems. As a result, we have very limited knowledge on species׳ behaviour and interactions in these communities and how they in turn influence community dynamics. Interactions such as competition or predation significantly affect community structure in vent communities, and video time-series have successfully been used to gain insights in biological interactions and species behaviour, including responses to short-term changes in temperature or feeding strategies. In this study, we combined in situ and ex situ approaches to characterise the behaviour and interactions among two key species encountered along the Mid-Atlantic Ridge (MAR): the shrimp Mirocaris fortunata and the crab Segonzacia mesatlantica. In situ, species small-scale distribution, interactions and behaviour were studied using the TEMPO observatory module deployed on the seafloor at the base of the active Eiffel Tower edifice in the Lucky Strike vent field as part of the EMSO-A{\c c}ores MoMAR observatory. TEMPO sampled 2min of video four times a day from July 2011 to April 2012. One week of observations per month was used for {\textquoteleft}long-term{\textquoteright} variations, and a full video data set was analysed for January 2012. In addition, observations of crab and shrimp individuals maintained for the first time under controlled conditions in atmospheric pressure (classic tank) and pressurised (AbyssBox) aquaria allowed better characterisation and description of the different types of behaviour and interactions observed in nature. While the identified in situ spatial distribution pattern was stable over the nine months, both species displayed a significant preference for mussel bed and anhydrite substrata, and preferentially occupied the area located directly in the fluid flow axis. The aggregation behaviour of M. fortunata resulted in the occurrence of numerous intraspecific interactions mainly involving the use of two pairs of sensory organs (antenna/antennule) and fleeing behaviours when in contact or close to individuals of S. mesatlantica. The higher level of passiveness observed in the ex situ artificial environment compared to the in situ environment was attributed to the lack of stimulation related to low densities of congeners and/or of sympatric species compared to the natural environment and the absence of continuous food supply, as both species displayed a significant higher level of activity during feeding time. This result emphasises the role of food supply as a driver of species distribution and behaviour. Direct in situ observations using cameras deployed on deep-sea observatories, combined with experimental set-up in pressurised aquaria, will help investigators understand the factors influencing community dynamics and species biology at vents as well as their underlying mechanisms.}, keywords = {32{\textdegree}16.3'W, 37{\textdegree}17'N, AbyssBox, Biological interactions, Deep-sea observatory, Eiffel Tower edifice, Experimental research, Feeding behaviour, Lucky Strike, Mid-Atlantic Ridge, Time series, Video imagery}, issn = {0967-0645}, doi = {https://doi.org/10.1016/j.dsr2.2015.04.008}, url = {http://www.sciencedirect.com/science/article/pii/S0967064515001113}, author = {Matabos Marjolaine and Cuvelier Daphn{\'e} and Brouard Johan and Bruce Shillito and Juliette Ravaux and Magali Zbinden and Barthelemy Dominique and Sarradin, Pierre-Marie and Sarrazin, Jozee} } @article {4165, title = {Biogeographical distribution of Rimicaris exoculata resident gut epibiont communities along the Mid-Atlantic Ridge hydrothermal vent sites.}, journal = {FEMS Microbiol Ecol}, volume = {91}, year = {2015}, month = {2015 Oct}, abstract = {

Rimicaris exoculata is a deep-sea hydrothermal vent shrimp whose enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystem megafauna along the Mid-Atlantic Ridge, regardless of contrasting geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow, TAG, Logatchev and Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (transmission electron microscopy, scanning electron microscopy and fluorescence in situ hybridization analyses). Filamentous epibionts inserted between the epithelial cell microvilli were observed on all examined samples. Results confirmed resident gut community affiliation to Deferribacteres, Mollicutes, Epsilonproteobacteria and to a lesser extent Gammaproteobacteria lineages. Still a single Deferribacteres phylotype was retrieved at all sites. Four Mollicutes-related operational taxonomic units were distinguished, one being only identified on Rainbow specimens. The topology of ribotype median-joining networks illustrated a community diversification possibly following demographic expansions, suggesting a more ancient evolutionary history and/or a larger effective population size at Rainbow. Finally, the gill chamber community distribution was also analysed through ribotype networks based on sequences from R. exoculata collected at the Rainbow, Snake Pit, TAG, Logatchev and Ashadze sites. Results allow the refining of hypotheses on the epibiont role and transmission pathways.

}, issn = {1574-6941}, doi = {10.1093/femsec/fiv101}, author = {Durand, Lucile and Roumagnac, Marie and Cueff-Gauchard, Val{\'e}rie and Jan, Cyrielle and Guri, Mathieu and Tessier, Claire and Haond, Marine and Crassous, Philippe and Magali Zbinden and Arnaud-Haond, Sophie and Cambon-Bonavita, Marie-Anne} } @article {3761, title = {Epsilonproteobacteria as gill epibionts of the hydrothermal vent gastropod Cyathermia naticoides (North East-Pacific Rise)}, journal = {Marine Biology}, volume = {162}, year = {2015}, month = {11 D{\'e}c 2014}, pages = {435-448}, abstract = {

Mollusks, and particularly gastropods, are one of the major taxonomic groups at vents. In these ecosystems, devoid of light, chemoautotrophic bacteria are at the base of the food web and symbiotic association between metazoa and these bacteria is numerous. Nevertheless, apart few {\textquotedblleft}large-size{\textquotedblright} well-known species, the {\textquotedblleft}small-size{\textquotedblright} gastropods (shell \<5\ mm), although very abundant, remain poorly studied regarding symbioses. We investigated here Cyathermia naticoides (War{\'e}n and Bouchet in Zool Scr 18(1), 1989), a small coiled gastropod found in abundance on the East Pacific Rise among Riftia pachyptila tubes, and usually inferred to graze on tubeworm bacterial cover, and/or filter feeding. Among mollusks, symbioses are well known in large species and almost exclusively rely on sulfide or methane-oxidizing proteobacterial endosymbionts, occurring within the host tissues in gill epithelial bacteriocytes. Combining several approaches (molecular biology, microscopy, stable isotopes analyses), we described here an unusual symbiosis, where autotrophic filamentous Epsilonproteobacteria are located extracellularly, at the base of host gill filaments. Numerous endocytotic lysosome-like structures were observed in the gill epithelium of the animal suggesting bacteria may contribute to its nutrition through intracellular digestion by gill cells. Additional food source by non-symbiotic proteobacteria grazed on R. pachyptila tubes could complete the diet. The possible role of temperature in the selection of Epsilon- vs Gammaproteobacterial partners is discussed.

}, url = {http://link.springer.com/article/10.1007/s00227-014-2591-7$\#$}, author = {Magali Zbinden and Marqu{\'e}, Lise and Sylvie M Gaudron and Juliette Ravaux and Nelly L{\'e}ger and S{\'e}bastien Duperron} } @article {6822, title = {How are microbial and detrital sources partitioned among and within gastropods species at East Pacific Rise hydrothermal vents?}, journal = {Marine Ecology}, volume = {36}, year = {2015}, pages = {18-34}, abstract = {Abstract For the last few decades, trophic ecology has usually been investigated by using stable isotopes. However, the isotopic signatures of potential food sources in hydrothermal vent ecosystems are often unknown and so their relative contribution to the consumers{\textquoteright} diet, as well as resource partitioning, are then difficult to estimate. Here, we used a recent Bayesian mixing model (stable isotope analysis in R, SIAR) based on δ13C and δ15N to estimate the contribution of multiple food sources to the diet of eight vent gastropods that can reach high densities at hydrothermal vents (Lepetodrilus elevatus, Lepetodrilus pustulosus, Lepetodrilus ovalis, Eulepetopsis vitrea, Cyathermia naticoides, Peltospira delicata, Peltospira operculata and Rhynchopelta concentrica). These species, known as primary consumers (mostly bacterivores and detritivores), were sampled on the South-East Pacific Rise at 17{\textdegree}25'\ S and the North-East Pacific Rise at 9{\textdegree}50'\ N and 12{\textdegree}50'\ N. Several potential food sources were sampled according to the gastropod habitat on the chimney wall, or mussel beds (proxies of Gammaproteobacteria form I RubisCO, Gammaproteobacteria form II RubisCO and Epsilonproteobacteria, biofilms of siboglinid and alvinellid tubes, biofilms of mussel shells and particulate organic matter). Some of these microbial and detrital sources were confirmed as present in the gut content of some small specimens by transmission electron microscopy. Distinct stable isotopic signatures of the potential food sources allowed calculation of their relative contributions to primary consumers{\textquoteright} diets. This revealed that gastropod species living on siboglinid or bathymodiolin habitats are usually generalists, feeding on various pools of microbial or detrital origins. For a particular habitat, sympatric gastropod species partition the food sources, thus avoiding being in competition. Only for the alvinellid habitat Peltospira spp. appeared to be more specialists as the choice of food sources is more reduced.}, keywords = {Chemosynthetic, gastropods, mixing model, Stable isotopes, trophic niche, vents}, doi = {10.1111/maec.12260}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/maec.12260}, author = {Sylvie M Gaudron and Marqu{\'e}, Lise and Eric Thi{\'e}baut and Riera, Pascal and Duperron, S{\'e}bastien and Magali Zbinden} } @article {3743, title = {An improved taxonomic sampling is a necessary but not sufficient condition for resolving inter-families relationships in Caridean decapods.}, journal = {Genetica}, volume = {143}, year = {2015}, month = {2015 Apr}, pages = {195-205}, abstract = {

During the past decade, a large number of multi-gene analyses aimed at resolving the phylogenetic relationships within Decapoda. However relationships among families, and even among sub-families, remain poorly defined. Most analyses used an incomplete and opportunistic sampling of species, but also an incomplete and opportunistic gene selection among those available for Decapoda. Here we test in the Caridea if improving the taxonomic coverage following the hierarchical scheme of the classification, as it is currently accepted, provides a better phylogenetic resolution for the inter-families relationships. The rich collections of the Mus{\'e}um National d{\textquoteright}Histoire Naturelle de Paris are used for sampling as far as possible at least two species of two different genera for each family or subfamily. All potential markers are tested over this sampling. For some coding genes the amplification success varies greatly among taxa and the phylogenetic signal is highly saturated. This result probably explains the taxon-heterogeneity among previously published studies. The analysis is thus restricted to the genes homogeneously amplified over the whole sampling. Thanks to the taxonomic sampling scheme the monophyly of most families is confirmed. However the genes commonly used in Decapoda appear non-adapted for clarifying inter-families relationships, which remain poorly resolved. Genome-wide analyses, like transcriptome-based exon capture facilitated by the new generation sequencing methods might provide a sounder approach to resolve deep and rapid radiations like the Caridea.

}, issn = {1573-6857}, doi = {10.1007/s10709-014-9807-0}, author = {Aznar-Cormano, L and Brisset, J and Chan, T-Y and Laure Corbari and Puillandre, N and Utge, J and Magali Zbinden and Zuccon, D and Samadi, S} } @article {6825, title = {Long-term maintenance and public exhibition of deep-sea hydrothermal fauna: The AbyssBox project}, journal = {Deep Sea Research Part II: Topical Studies in Oceanography}, volume = {121}, year = {2015}, pages = {137 - 145}, abstract = {The AbyssBox project aims to provide the first permanent public exhibition of live deep-sea hydrothermal fauna maintained at in situ pressure. AbyssBox is a pressurized aquarium designed to function permanently. Here we present details of the project after the public exhibition functioned for more than three years at Oc{\'e}anopolis aquarium in Brest, France. We also describe the AbyssBox pressure aquarium, and provide data and observations on vent shrimp (Mirocaris fortunata) and crabs (Segonzacia mesatlantica) that were sampled from 1700m depth at the Lucky Strike vent field (Mid-Atlantic Ridge) during different cruises. While mortalities exceeded 50\% during the first days following sampling, the remaining animals appeared to acclimate fairly well. Some crabs have now been kept for more than 2 years, and some shrimp have spent more than 3 years in captivity. Primarily designed for a public exhibition, the AbyssBox is already used for scientific purposes, since it provides one of the most effective tools for long-term rearing of deep-sea fauna. AbyssBox is a first step towards maintaining a variety of deep-sea fauna year-round at in situ pressure, which will serve both scientific and public interests.}, keywords = {AbyssBox, Aquaria, Aquariology, Deep waters, Hydrostatic Pressure, Hydrothermal Vents, Public exhibition, Scientific experiments}, issn = {0967-0645}, doi = {https://doi.org/10.1016/j.dsr2.2015.05.002}, url = {http://www.sciencedirect.com/science/article/pii/S0967064515001460}, author = {Bruce Shillito and Juliette Ravaux and Sarrazin, Jozee and Magali Zbinden and Sarradin, Pierre-Marie and Barthelemy Dominique} }