@article {9192, title = {Data quality control considerations in multivariate environmental monitoring: experience of the French coastal network SOMLIT}, journal = {Frontiers in Marine Science}, volume = {10}, year = {2023}, month = {Feb-04-2025}, doi = {doi.org/10.3389/fmars.2023.1135446}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2023.1135446/full}, author = {Breton, Elsa and Savoye, Nicolas and Rimmelin-Maury, Peggy and Sautour, Benoit and Goberville, Eric and Lheureux, Arnaud and Cariou, Thierry and Ferreira, Sophie and Agogue, Helene and Alliouane, Samir and Aubert, Fabien and Aubin, S{\'e}bastien and Berthebaud, Eric and Blayac, Hadrien and Blondel, Lucie and Boulart, C{\'e}dric and Bozec, Yann and Bureau, Sarah and Caillo, Arnaud and Cauvin, Arnaud and Cazes, Jean-Baptiste and Chasselin, L{\'e}o and Pascal Claquin and Conan, Pascal and Cordier, Marie-Ange and Costes, Laurence and Crec{\textquoteright}hriou, Romain and Crispi, Olivier and Crouvoisier, Muriel and David, Val{\'e}rie and Del Amo, Yolanda and De Lary, Hortense and Delebecq, Gaspard and Devesa, J{\'e}r{\'e}my and Domeau, Aur{\'e}lien and Durozier, Maria and Emery, Claire and Eric Feunteun and Juliette Fauchot and Gentilhomme, Val{\'e}rie and Geslin, Sandrine and Giraud, M{\'e}lanie and Granger{\'e}, Karine and Gr{\'e}gori, Gerald and Grossteffan, Emilie and Gueux, Aurore and Guillaudeau, Julien and Guillou, Ga{\"e}l and Harrewyn, Manon and Jolly, Orianne and Jude-Lemeilleur, Florence and Labatut, Paul and Labourdette, Nathalie and Lachauss{\'e}e, Nicolas and Lafont, Michel and Lagadec, V{\'e}ronique and Lambert, Christophe and Lamoureux, Jezebel and Lanceleur, Laurent and Lebreton, Beno{\^\i}t and Lecuyer, Eric and Lemeille, David and Leredde, Yann and Leroux, C{\'e}dric and Leynaert, Aude and L{\textquoteright}Helguen, St{\'e}phane and Li{\'e}nart, Camilla and Mac{\'e}, Eric and Maria, Eric and Marie, Barbara and Marie, Dominique and Mas, S{\'e}bastien and Mendes, Fabrice and Mornet, Line and Mostajir, Behzad and Mousseau, Laure and Nowaczyk, Antoine and Nunige, Sandra and Parra, Ren{\'e} and Paulin, Thomas and Pecqueur, David and Petit, Franck and Pineau, Philippe and Raimbault, Patrick and Rigaut-Jalabert, Fabienne and Salmeron, Christophe and Salter, Ian and Sauriau, Pierre-Guy and Seuront, Laurent and Sultan, Emmanuelle and Vald{\`e}s, R{\'e}mi and Vantrepotte, Vincent and Vidussi, Francesca and Voron, Florian and Vuillemin, Renaud and Zudaire, Laurent. and Garcia, Nicole} } @article {9052, title = {Physiological conditions favorable to domoic acid production by three Pseudo-nitzschia species}, journal = {Journal of Experimental Marine Biology and Ecology}, volume = {559}, year = {2023}, month = {Jan-02-2023}, pages = {151851}, issn = {00220981}, doi = {10.1016/j.jembe.2022.151851}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0022098122001599}, author = {Aurore Sauvey and Pascal Claquin and Le Roy, Bertrand and Jolly, Orianne and Juliette Fauchot} } @article {9129, title = {Interactions between Filter-Feeding Bivalves and Toxic Diatoms: Influence on the Feeding Behavior of Crassostrea gigas and Pecten maximus and on Toxin Production by Pseudo-nitzschia}, journal = {Toxins}, volume = {13}, year = {2021}, month = {Jan-08-2021}, pages = {577}, doi = {10.3390/toxins13080577}, url = {https://www.mdpi.com/2072-6651/13/8/577}, author = {Sauvey, Aurore and Denis, Fran{\c c}oise and H{\'e}garet, H{\'e}l{\`e}ne and Le Roy, Bertrand and Lelong, Christophe and Jolly, Orianne and Pavie, Marie and Juliette Fauchot} } @article {6656, title = {Differential Influence of Life Cycle on Growth and Toxin Production of three Pseudo-nitzschia Species (Bacillariophyceae)}, journal = {Journal of Phycology}, volume = {55}, year = {2019}, pages = {1126-1139}, abstract = {We used a multistrain approach to study the intra- and interspecific variability of the growth rates of three Pseudo-nitzschia species {\textendash} P. australis, P. fraudulenta, and P. pungens {\textendash} and of their domoic acid (DA) production. We carried out mating and batch experiments to investigate the respective effects of strain age and cell size, and thus the influence of their life cycle on the physiology of these species. The cell size {\textendash} life cycle relationship was characteristic of each species. The influence of age and cell size on the intraspecific variability of growth rates suggests that these characteristics should be considered cautiously for the strains used in physiological studies on Pseudo-nitzschia species. The results from all three species do not support the hypothesis of a decrease in DA production with time since isolation from natural populations. In P. australis, the cellular DA content was rather a function of cell size. More particularly, cells at the gametangia stage of their life cycle contained up to six times more DA than smaller or larger cells incapable of sexual reproduction. These findings reveal a link between P. australis life cycle and cell toxicity. This suggest that life cycle dynamics in Pseudo-nitzschia natural populations may influence bloom toxicity. {\textcopyright} 2019 Phycological Society of America}, issn = {00223646}, doi = {10.1111/jpy.12898}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12898}, author = {Aurore Sauvey and Pascal Claquin and Le Roy, Bertrand and Le Gac, Mickael and Juliette Fauchot} } @article {8745, title = {Differential Influence of Life Cycle on Growth and Toxin Production of three Pseudo-nitzschia species (Bacillariophyceae)}, journal = {Journal of Phycology}, volume = {55}, year = {2019}, month = {Mar-10-2021}, pages = {1126 - 1139}, issn = {0022-3646}, doi = {10.1111/jpy.v55.510.1111/jpy.12898}, url = {https://onlinelibrary.wiley.com/toc/15298817/55/5}, author = {Aurore Sauvey and Pascal Claquin and Bertrand Le Roy and Le Gac, Mickael and Juliette Fauchot}, editor = {Kroth, P.} } @article {Lema2019731, title = {Inter- and Intra-Specific Transcriptional and Phenotypic Responses of Pseudo-nitzschia under Different Nutrient Conditions}, journal = {Genome Biology and Evolution}, volume = {11}, number = {3}, year = {2019}, note = {cited By 1}, pages = {731-747}, publisher = {Oxford University Press}, abstract = {Untangling thefunctionalbasis of divergencebetweenclosely relatedspecies is a steptowardunderstanding speciesdynamicswithin communities at both the evolutionary and ecological scales. We investigated cellular (i.e., growth, domoic acid production, and nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatomgenus Pseudo-nitzschia. Threemain resultswere obtained. First, strains fromthe same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intraspecific diversity to investigate functional divergence between species. Second, a major exception to the first findingwas a strain recently isolated fromthe natural environment and displaying contrasting gene expression patterns related to cell motility and domoic acid production. This result illustrated the profound modifications thatmay occurwhen transferring a cell fromthe natural to the in vitro environment and asks for future studies to better understand the influence of culture duration and life cycleon expression patterns. Third, transcriptomic responsesweremore similarbetween the two speciesdisplaying similar ecology in situ, irrespective of the genetic distance. This was especially true formolecular responses related to TCA cycle, photosynthesis, and nitrogen metabolism. However, transcripts related to phosphate uptake were variable between species. It highlighted the importance of considering both overall genetic distance and ecological divergence to explain functional divergence between species. {\textcopyright} The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.}, keywords = {analogs and derivatives, Biological Evolution, comparative study, Diatom, Diatoms, Domoic acid, evolution, kainic acid, Metabolism, Multigene Family, Nutrients, Phenotype, Physiology}, issn = {17596653}, doi = {10.1093/gbe/evz030}, url = {https://academic.oup.com/gbe/article/11/3/731/5332980}, author = {Lema, Kimberley A. and Metegnier, G. and Qu{\'e}r{\'e}, J. and Latimier, Marie and Agn{\`e}s Youenou and Lambert, Christophe and Juliette Fauchot and Le Gac, Mickael and Costantini, M.} } @article {5152, title = {Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia: phosphate an important factor}, journal = {Harmful Algae}, volume = {64}, year = {2017}, pages = {11-19}, abstract = {

The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton
blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and
interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of
cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens
and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in
phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA
concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial
concentration of nitrogen and silicate combined, with a higher phosphate concentration resulted in low
cell densities, but without high DA production. Inter-species effects were also observed in DA production,
where P. australis represented the most toxigenic species of all. Intra-specific variations were only
moderate, except for a recently isolated P. australis strain, suggesting the influence of time since isolation
on the physiology and DA production of Pseudo-nitzschia species. Overall, the lack of strong interaction
between environmental and genotypic factors showed that the various genotypes investigated did not
extensively diverge in their ability to respond (in terms of DA production and cell densities) to contrasting
nutrient supply.

}, doi = {http://dx.doi.org/10.1016/j.hal.2017.03.001}, url = {https://www.sciencedirect.com/science/article/pii/S1568988316303687}, author = {Lema, Kimberley A. and Latimier, Marie and N{\'e}zan, Elisabeth and Juliette Fauchot and Le Gac, Mickael} } @article {Thorel2017192, title = {Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate domoic acid production in the Bay of Seine (France)}, journal = {Harmful Algae}, volume = {68}, year = {2017}, note = {cited By 15}, pages = {192-205}, publisher = {Elsevier B.V.}, abstract = {The population dynamics of different Pseudo-nitzschia species, along with particulate domoic acid (pDA) concentrations, were studied from May 2012 to December 2013 in the Bay of Seine (English Channel, Normandy). While Pseudo-nitzschia spp. blooms occurred during the two years of study, Pseudo-nitzschia species diversity and particulate domoic acid concentrations varied greatly. In 2012, three different species were identified during the spring bloom (P. australis, P. pungens and P. fraudulenta) with high pDA concentrations (\~{}1400 ng l-1) resulting in shellfish harvesting closures. In contrast, the 2013 spring was characterised by a P. delicatissima bloom without any toxic event. Above all, the results show that high pDA concentrations coincided with the presence of P. australis and with potential silicate limitation (Si:N \< 1), while nitrate concentrations were still replete. The contrasting environmental conditions between 2012 and 2013 highlight different environmental controls that might favour the development of either P. delicatissima or P. australis. This study points to the key role of Pseudo-nitzschia diversity and cellular toxicity in the control of particulate domoic acid variations and highlights the fact that diversity and toxicity are influenced by nutrients, especially nutrient ratios. {\textcopyright} 2017 Elsevier B.V.}, keywords = {analogs and derivatives, analysis, Bay, Bays, Biodiversity, chemistry, Diatom, Diatoms, Domoic acid, France, Geography, kainic acid, Nitrates, nitric acid derivative, particulate matter, phosphate, Phosphates, Physiology, Phytoplankton, Principal Component Analysis, season, Seasons, species difference, Species Specificity, time factor, Time Factors}, issn = {15689883}, doi = {10.1016/j.hal.2017.07.005}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028543709\&doi=10.1016\%2fj.hal.2017.07.005\&partnerID=40\&md5=def23b37b0d16a1ae7ab65a7ef2b940b}, author = {Thorel, Maxine and Pascal Claquin and Mathilde Schapira and Romain Le Gendre and Riou, Philippe and Didier Goux and Bertrand Le Roy and Raimbault, V and Deton-Cabanillas, A.-F. and Bazin, Pauline and Kientz-Bouchart, Val{\'e}rie and Juliette Fauchot} } @article {5143, title = {Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate domoic acid production in the Bay of Seine (France)}, journal = {Harmful Algae}, volume = {68}, year = {2017}, pages = {192 - 205}, abstract = {

The population dynamics of different Pseudo-nitzschia species, along with particulate domoic acid (pDA) concentrations, were studied from May 2012 to December 2013 in the Bay of Seine (English Channel, Normandy). While Pseudo-nitzschia spp. blooms occurred during the two years of study, Pseudo-nitzschia species diversity and particulate domoic acid concentrations varied greatly. In 2012, three different species were identified during the spring bloom (P. australis, P. pungens and P. fraudulenta) with high pDA concentrations (\~{}1400\ ng\ l-1) resulting in shellfish harvesting closures. In contrast, the 2013 spring was characterised by a P. delicatissima bloom without any toxic event. Above all, the results show that high pDA concentrations coincided with the presence of P. australis and with potential silicate limitation (Si:N\ \<\ 1), while nitrate concentrations were still replete. The contrasting environmental conditions between 2012 and 2013 highlight different environmental controls that might favour the development of either P. delicatissima or P. australis. This study points to the key role of Pseudo-nitzschia diversity and cellular toxicity in the control of particulate domoic acid variations and highlights the fact that diversity and toxicity are influenced by nutrients, especially nutrient ratios.

}, keywords = {Bloom dynamics, Domoic acid, English Channel, Nutrient ratios, Pseudo-nitzschia Species diversity}, issn = {1568-9883}, doi = {https://doi.org/10.1016/j.hal.2017.07.005}, url = {http://www.sciencedirect.com/science/article/pii/S1568988317300148}, author = {Thorel, Maxine and Pascal Claquin and Mathilde Schapira and Romain Le Gendre and Riou, Philippe and Goux, Didier and Le Roy, Bertrand and Raimbault, V and Deton-Cabanillas, Anne-Flore and Bazin, Pauline and Kientz-Bouchart, Val{\'e}rie and Juliette Fauchot} } @article {3413, title = {Interactive effects of irradiance and temperature on growth and domoic acid production of the toxic diatom Pseudo-nitzschia australis (Bacillariophyceae)}, journal = {Harmful Algae}, volume = {39}, year = {2014}, pages = {232-241}, abstract = {The influence of temperature and light on the growth, physiology and domoic acid (DA) production of the pennate toxic diatom Pseudo-nitzschia australis isolated from the English Channel was studied in semicontinuous culture. The effects of eight irradiances (35{\textendash}400 mmol photons m2 s-1) and a temperature gradient (3.5{\textendash}25.5 8C) were investigated. The highest growth rates (0.47{\textendash}0.83 d-1) were observed between 18.6 and 13.5 8C, with an optimum irradiance for growth at 100 mmol photons m2 s-1. A decrease in the optimum growth temperature was observed with an increase in irradiance. Cell chlorophyll a content decreased at both low and high extreme temperatures and high irradiances. Stable and high values of Fv/Fm ratios below 21.8C highlight the great acclimation capacity of P. australis, which may explain its wide biogeographic distribution. The strain studied produced DA without nutrient limitation and during exponential growth although at lower levels than in other studies (0.15{\textendash} 2.0 pg DA cell-1). Results underline the importance of light{\textendash}temperature interactions for growth and DA production. Toxin production increased with increasing temperature and light, and DA production increased exponentially with the growth rate. These results show that DA is not only produced under nutrient stress or by unhealthy cells in P. australis. This study underlines the need to take the ability of P. australis to produce DA during the exponential growth into consideration to understand the link between toxin production and bloom dynamics. These results will not only help explore the processes involved, but also help parameterize future models of growth and DA production especially for P. australis.}, keywords = {Domoic acid, Growth Irradiance, Pseudo-nitzschia australis, Temperature}, author = {Thorel, Maxine and Juliette Fauchot and Morelle, J{\'e}r{\^o}me and Raimbault, V and Le Rou, B and Miossec, C and Kientz-Bouchard, V and Pascal Claquin} }