@article {7035, title = {Identification and structural characterization of the factors involved in vitellogenesis and its regulation in the African Osteoglossiforme of aquacultural interest Heterotis niloticus (Cuvier, 1829)}, journal = {General and Comparative Endocrinology}, year = {2020}, month = {Jan-06-2020}, pages = {113532}, issn = {00166480}, doi = {10.1016/j.ygcen.2020.113532}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648020302859}, author = {Daniel Koua, N{\textquoteright}Zi and Jesus Nu{\~n}ez-Rodriguez and Orjuela, Julie and C{\'e}line Zatylny-Gaudin and Dubos, Marie-Pierre and Bernay, Beno{\^\i}t and Pontin, Julien and Corre, Erwan and Henry, Jo{\"e}l} } @article {7036, title = {Shedding light on the migratory patterns of the Amazonian goliath catfish, Brachyplatystoma platynemum , using otolith 87 Sr/ 86 Sr analyses}, journal = {Aquatic Conservation: Marine and Freshwater Ecosystems}, volume = {29}, year = {2019}, month = {Aug-03-2020}, pages = {397 - 408}, issn = {1052-7613}, doi = {10.1002/aqc.v29.310.1002/aqc.3046}, url = {https://onlinelibrary.wiley.com/toc/10990755/29/3}, author = {Hauser, Marilia and Doria, Carolina R.C. and Santos, Roberto V. and Garc{\'\i}a-Vasquez, Aurea and Marc Pouilly and Pecheyran, Christophe and Ponzevera, Emmanuel and Torrente-Vilara, Gislene and B{\'e}rail, Sylvain and Panfili, Jacques and Darnaude, Audrey and Renno, Jean-Fran{\c c}ois and Garc{\'\i}a-D{\'a}vila, Carmen and Jesus Nu{\~n}ez-Rodriguez and Ferraton, Franck and Vargas, Gladys and Duponchelle, Fabrice} } @article {7037, title = {Metabarcoding by capture using a single COI probe (MCSP) to identify and quantify fish species in ichthyoplankton swarms}, journal = {PLOS ONE}, volume = {13}, year = {2018}, month = {Dec-09-2018}, pages = {e0202976}, doi = {10.1371/journal.pone.0202976}, url = {https://dx.plos.org/10.1371/journal.pone.0202976}, author = {Mariac, C. and Vigouroux, Y. and Duponchelle, F. and Garc{\'\i}a-D{\'a}vila, C and Jesus Nu{\~n}ez-Rodriguez and Desmarais, E. and Renno, J.F.}, editor = {Hajibabaei, Mehrdad} } @book {5650, title = {Peces de consumo de la Amazon{\'\i}a Peruana}, year = {2018}, pages = {218}, edition = {Instituto de Investigaciones de la Amazon{\'\i}a Peruana (IIAP), Iquitos, Per{\'u}}, author = {Garc{\'\i}a-D{\'a}vila, C and Sanchez, H. and Flores, M and Mejia, J. and Angulo, C. and Castro-Ruiz, D. and Estivals, G. and Garcia, Aurea and Vargas, G. and Nolorbe, C. and Jesus Nu{\~n}ez-Rodriguez and Mariac, C{\'e}dric and Fabrice Duponchelle and Renno, Jean-Francois} } @article {7034, title = {Use of an acoustic telemetry array for fine scale fish behaviour assessment of captive Paiche, Arapaima gigas, breeders}, journal = {Aquaculture Research}, volume = {49}, year = {2018}, month = {Jan-06-2018}, pages = {2296 - 2304}, doi = {10.1111/are.2018.49.issue-610.1111/are.13692}, url = {http://doi.wiley.com/10.1111/are.2018.49.issue-6}, author = {Jesus Nu{\~n}ez-Rodriguez and Vela D{\'\i}az, Antonia and Bazan-Albitez, Roger and Rebaza Alfaro, Carmela and Koua, Daniel and N{\'u}{\~n}ez, Lucie and Testi, Baptiste and Renno, Jean-Francois and Duponchelle, Fabrice and Pella, Herv{\'e}} } @article {5628, title = {Use of an acoustic telemetry array for fine scale fish behaviour assessment of captive Paiche, Arapaima gigas, breeders}, journal = {Aquaculture Research }, volume = {49}, year = {2018}, pages = {2296-2304}, abstract = {

As\ Arapaima gigas\ is one of the most valuable species for the growing production of Amazonian aquaculture, knowledge of its reproductive behaviour and its application to increase reproduction success in captivity is of great importance as no hormonal spawning induction technique exists for this species. An acoustic positioning system (LOTEK Inc.) was used to observe the interactions of adult fish to better understand the formation of mating pairs. Fish were placed in a 4,500\ m2\ aquaculture pond over a 6-month period in the IIAP field station of Pucallpa, Per{\'u}. This paper describes the methodological protocols used to set up and test the hydrophone array and presents the methodology used for the analysis of the huge amount of collected data. This methodology is illustrated by the analysis of a 6-day period for a mating pair that showed a spawning event. The results indicated that male and female occupied mostly one preferential area in one pond edge where the nesting area is located. Different activity patterns were observed during the spawning event, with male and female being closer during the spawning day. The results also showed that male travelled less distance than female during the studied period. Finally these results demonstrated the suitability of such equipment to monitor fish interactions at fine spatial (sub meter) and temporal (5\ s) scales in confined environments like aquaculture ponds.

}, doi = {doi.org/10.1111/are.13692}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/are.13692}, author = {Jesus Nu{\~n}ez-Rodriguez and Vela Diaz, A. and Bazan-Albitez, R and Koua, D and Nu{\~n}ez L. and Testi, B. and Renno, Jean-Francois and Duponchelle, F and Pella, H} } @article {4796, title = {DNA Metabarcoding of Amazonian Ichthyoplankton Swarms}, journal = {PLoSOne}, volume = {Jan 17;12}, year = {2017}, month = {01/2017}, pages = {:e0170009}, abstract = {

Tropical rainforests harbor extraordinary biodiversity. The Amazon basin is thought to hold 30\% of all river fish species in the world. Information about the ecology, reproduction, and recruitment of most species is still lacking, thus hampering fisheries management and successful conservation strategies. One of the key understudied issues in the study of population dynamics is recruitment. Fish larval ecology in tropical biomes is still in its infancy owing to identification difficulties. Molecular techniques are very promising tools for the identification of larvae at the species level. However, one of their limits is obtaining individual sequences with large samples of larvae. To facilitate this task, we developed a new method based on the massive parallel sequencing capability of next generation sequencing (NGS) coupled with hybridization capture. We focused on the mitochondrial marker cytochrome oxidase I (COI). The results obtained using the new method were compared with individual larval sequencing. We validated the ability of the method to identify Amazonian catfish larvae at the species level and to estimate the relative abundance of species in batches of larvae. Finally, we applied the method and provided evidence for strong temporal variation in reproductive activity of catfish species in the Ucayal{\'\i} River in the Peruvian Amazon. This new time and cost effective method enables the acquisition of large datasets, paving the way for a finer understanding of reproductive dynamics and recruitment patterns of tropical fish species, with major implications for fisheries management and conservation.

}, author = {Maggia, M. E. and Vigouroux, Y. and Renno, Jean-Francois and Fabrice Duponchelle and Desmarais, E and Jesus Nu{\~n}ez-Rodriguez and Garc{\'\i}a-D{\'a}vila, C and Carvajal-Vallejos, F M and Paradis, Emmanuel and Martin, Jean-Fran{\c c}ois and Mariac, C{\'e}dric} } @article {4597, title = {Parental influence in relation to growth and survival in larval families of Doncella Pseudoplatystoma punctifer}, journal = {Folia Amaz{\'o}nica}, volume = {25}, year = {2016}, pages = {77-82}, author = {Castro-Ruiz, D and Baras, Etienne and Fern{\'a}ndez, C and Sophie Qu{\'e}rouil and Chota-Macuyama, Werner and F. Duponchelle and Renno, J-F and Darias, Maria and Garc{\'\i}a-D{\'a}vila, C and Jesus Nu{\~n}ez-Rodriguez} } @article {4280, title = {Trans-Amazonian natal homing in giant catfish}, journal = {Journal of Applied Ecology}, volume = {53}, year = {2016}, pages = {1511-1520}, abstract = {

1.Knowledge of fish migration is a prerequisite to sustainable fisheries management and preservation, especially in large international river basins. In particular, understanding whether a migratory lifestyle is compulsory or facultative, and whether adults home to their natal geographic area is paramount to fully appraise disruptions of longitudinal connectivity resulting from damming.2.In the Amazon, the large migratory catfishes of the Brachyplatystoma genus are apex predators of considerable interest for fisheries. They are believed to use the entire length of the basin to perform their life cycle, with hypothesized homing behaviours. Here we tested these hypotheses, using the emblematic B. rousseauxii as a model species.3.We sampled adults close to major breeding areas in the Amazon basin (upper Madeira and upper Amazonas) and assessed their lifetime movements by measuring variations in 87Sr/86Sr along transverse sections of their otoliths (ear stones) using laser ablation multicollector mass spectrometry (LA-MC-ICPMS).4.We demonstrate that larvae migrate downstream from the Andean piedmont to the lower Amazon, where they grow over a protracted period before migrating upstream as adults. Contrary to prevailing inferences, not all fish spend their nursery stages in the Amazon estuary. By contrast, the passage in the lower or central Amazon seems an obligate part of the life cycle. We further evidence that most adults home to their natal geographic area within the Madeira sub-basin. Such long-distance natal homing is exceptional in purely freshwater fishes.5.Synthesis and applications. By using otolith microchemistry, we were able to demonstrate a seemingly compulsory basin-wide migratory life cycle of large Amazonian catfishes. This makes them the organisms performing the longest migrations (\>8000 km) in fresh waters. This exceptional life history is already jeopardized by two dams recently built in the Madeira River, which block a major migration route and access to a substantial part of their spawning grounds. Major impacts can be anticipated from the current and forthcoming hydroelectric development in the Amazon basin, not only on the populations and fisheries of this apex predator, but also on Amazonian food webs through trophic cascades.This article is protected by copyright. All rights reserved.

}, keywords = {87Sr/86Sr ratios, Amazon, anthropogenic activities, Brachyplatystoma spp, freshwater fish, giant catfish, hydroelectric dams, Migration, otoliths}, issn = {1365-2664}, doi = {10.1111/1365-2664.12665}, url = {http://dx.doi.org/10.1111/1365-2664.12665}, author = {Fabrice Duponchelle and Marc Pouilly and Pecheyran, Christophe and Hauser, Marilia and Renno, Jean-Francois and Panfili, Jacques and Darnaude, Audrey M. and Garc{\'\i}a-Vasquez, Aurea and Carvajal-Vallejos, Fernando and Carmen Garc{\'\i}a-D{\'a}vila and Doria, Carolina and B{\'e}rail, Sylvain and Donard, Ariane and Sondag, Francis and Santos, Roberto V. and Jesus Nu{\~n}ez-Rodriguez and Point, David and Labonne, Maylis and Baras, Etienne} } @article {4823, title = {Comparative environmental performance of artisanal and commercial feed use in Peruvian freshwater aquaculture}, journal = {Aquaculture}, volume = {435}, year = {2015}, pages = {52-66}, type = {Journal Article}, issn = {0044-8486}, doi = {http://dx.doi.org/10.1016/j.aquaculture.2014.08.001}, url = {http://www.sciencedirect.com/science/article/pii/S0044848614003925}, author = {Avad{\'\i}, Angel and Pelletier, Nathan and Aubin, Jo{\"e}l and Ralite, St{\'e}phane and Jesus Nu{\~n}ez-Rodriguez and Fr{\'e}on, Pierre} } @article {4202, title = {Influence of dietary protein and lipid levels on growth performance and the incidence of cannibalism in Pseudoplatystoma punctifer~(Castelnau, 1855) larvae and early juveniles}, journal = {Journal of Applied Ichthyology}, volume = {31}, year = {2015}, pages = {74{\textendash}82}, abstract = {

The aim of the study was to evaluate the influence of different dietary protein and lipid levels and their ratios on larval growth, survival and the incidence of cannibalism in Pseudoplatystoma punctifer. Larvae were raised in a recirculation system from 3 to 26\ days post-fertilization (dpf) (2{\textendash}25\ days post hatching, dph) at an initial density of 40 larvae L-1, 27.8\ {\textpm}\ 0.65{\textdegree}C and 0L\ :\ 24D photoperiod. Larvae were fed from 4 to 12 dpf with Artemia nauplii and weaned onto four different compound diets from 13 dpf within 3\ days, then fed exclusively with these diets until 26 dpf. These diets contained 30\ :\ 15, 30\ :\ 10, 45\ :\ 15 or 45\ :\ 10 protein\ :\ lipid (P\ :\ L) (in \% of dry matter) levels. A control group was fed Artemia nauplii until 17 dpf and weaned thereafter with the 45P\ :\ 10L compound diet. The experiment was carried out in triplicate. Results showed higher growth and survival rates and lower incidence of cannibalism in the group fed the 45P\ :\ 15L diet than in the other treatments. Differences in larval survival and growth performance were associated with the higher protein and lipid content rather than the protein\ :\ lipid ratio of this diet. When comparing diets with the same protein level, the increase in dietary lipid led to an improvement in growth, suggesting that energy from lipids spares protein for growth in P.\ punctifer fingerlings. An Artemia feeding period longer than 12 dpf did not improve larval growth or survival.

}, issn = {1439-0426}, doi = {10.1111/jai.12978}, url = {http://dx.doi.org/10.1111/jai.12978}, author = {Darias, Maria and Castro-Ruiz, D and Estivals, G and Quazuguel, Patrick and Fern{\'a}ndez, C and Jesus Nu{\~n}ez-Rodriguez and Clota, F. and Gilles, S and Garc{\'\i}a-D{\'a}vila, C and Gisbert, E. and Cahu, Chantal L} } @article {4398, title = {Movement patterns and home range of wild and re-stocked Arapaima gigas (Schinz, 1822) monitored by radio-telemetry in Lake Imiria, Peru}, journal = {Journal of Applied Ichthyology}, volume = {31}, year = {2015}, pages = {10{\textendash}18}, issn = {1439-0426}, doi = {10.1111/jai.12972}, url = {http://dx.doi.org/10.1111/jai.12972}, author = {Jesus Nu{\~n}ez-Rodriguez and Fabrice Duponchelle and Cotrina-Doria, M. and Renno, Jean-Francois and Ch{\'a}vez Veintemilla, C and Rebaza, C. and Deza, S. and Garc{\'\i}a-D{\'a}vila, C and Chu-Koo, F and Salvador Tello and Baras, Etienne} } @article {4822, title = {Rearing of the Amazon catfish Pseudoplatystoma punctifer (Castelnau, 1855): weaning with dry and moist diets}, journal = {Journal of Applied Ichthyology}, volume = {31}, year = {2015}, pages = {83-87}, type = {Journal Article}, issn = {1439-0426}, doi = {10.1111/jai.12979}, url = {http://dx.doi.org/10.1111/jai.12979}, author = {Fern{\'a}ndez-M{\'e}ndez, C. and Frank David and Darias, Maria and Castro-Ruiz, Diana and Jesus Nu{\~n}ez-Rodriguez} } @article {4399, title = {Using barcoding of larvae for investigating the breeding seasons of pimelodid catfishes from the Mara{\~n}on, Napo and Ucayali rivers in the Peruvian Amazon}, journal = {Journal of Applied Ichthyology}, volume = {31}, year = {2015}, pages = {40{\textendash}51}, issn = {1439-0426}, doi = {10.1111/jai.12987}, url = {http://dx.doi.org/10.1111/jai.12987}, author = {Garc{\'\i}a-D{\'a}vila, C. and Castro-Ruiz, Diana and Renno, Jean-Francois and Chota-Macuyama, Werner and Carvajal-Vallejos, Fernando and Sanchez, H. and Angulo, C. and Nolorbe, C. and Alvarado, J. and Estivals, G and Jesus Nu{\~n}ez-Rodriguez and Fabrice Duponchelle} } @article {4392, title = {Variations in reproductive strategy of the silver Arowana, Osteoglossum bicirrhosum Cuvier, 1829 from four sub-basins of the Peruvian Amazon}, journal = {Journal of Applied Ichthyology }, volume = {31}, year = {2015}, pages = {19-30}, author = {Fabrice Duponchelle and Ruiz-Arce Adela and Waty, A. and Garc{\'\i}a-Vasquez, Aurea and Renno, J-F and Chu-Koo, F and Garc{\'\i}a-D{\'a}vila, C. and Vargas, Gladys and Salvador Tello and Ortiz, A. and Pinedo, R. and Manzanares V{\'a}squez, R. and Jesus Nu{\~n}ez-Rodriguez} } @article {4824, title = {Determinaci{\'o}n del sexo en arahuana Osteoglossum bicirrhosum mediante la detecci{\'o}n de los niveles plasm{\'a}ticos de vitelogenina}, journal = {Ciencia Amaz{\'o}nica}, volume = {4}, year = {2014}, pages = {151-159}, type = {Journal Article}, keywords = {Arahuana, Sexaje, Vitellogenine}, doi = {10.22386/ca.v4i2.81}, author = {Ram{\'\i}rez-Arrarte, Pedro and Jesus Nu{\~n}ez-Rodriguez and Chu-Koo, Fred} } @article {3445, title = {Genetic structure in the Amazonian catfish Brachyplatystoma rousseauxii: influence of life history strategies.}, journal = {Genetica}, volume = {142}, year = {2014}, month = {2014 Aug}, pages = {323-36}, abstract = {

The Dorado or Plateado (Gilded catfish) Brachyplatystoma rousseauxii (Pimelodidae, Siluriformes) is a commercially valuable migratory catfish performing the largest migration in freshwaters: from the Amazonian headwaters in the Andean foothills (breeding area) to the Amazon estuary (nursery area). In spite of its importance to inform management and conservation efforts, the genetic variability of this species has only recently begun to be studied. The aim of the present work was to determine the population genetic structure of B. rousseauxii in two regions: the Upper Madera Basin (five locations in the Bolivian Amazon) and the Western Amazon Basin (one regional sample from the Uyucal{\'\i}-Napo-Mara{\~n}on-Amazon basin, Peru). Length polymorphism at nine microsatellite loci (284 individuals) was used to determine genetic variability and to identify the most probable panmictic units (using a Bayesian approach), after a significant departure from Hardy-Weinberg equilibrium was observed in the overall dataset (Western Amazon\ +\ Upper Madera). Bayesian analyses revealed at least three clusters in admixture in the five locations sampled in the Bolivian Amazon, whereas only two of these clusters were observed in the Western Amazon. Considering the migratory behaviour of B. rousseauxii, different life history strategies, including homing, are proposed to explain the cluster distribution. Our results are discussed in the light of the numerous threats to the species survival in the Madera basin, in particular dam and reservoir construction.

}, issn = {1573-6857}, doi = {10.1007/s10709-014-9777-2}, author = {Carvajal-Vallejos, F M and Fabrice Duponchelle and Desmarais, E and Cerqueira, F and Sophie Qu{\'e}rouil and Jesus Nu{\~n}ez-Rodriguez and Garc{\'\i}a, C and Renno, J-F} } @article {3250, title = {Histological development of the digestive system of the Amazonian pimelodid catfish Pseudoplatystoma punctifer.}, journal = {Animal}, volume = {8}, year = {2014}, month = {2014 Nov}, pages = {1765-76}, abstract = {

The organogenesis of the digestive system was described in the Amazonian pimelodid catfish species Pseudoplatystoma punctifer from hatching (3.5 mm total length, TL) to 41 days post-fertilization (dpf) (58.1 mm TL) reared at 28{\textdegree}C. Newly hatched larvae showed a simple digestive tract, which appeared as a straight undifferentiated and unfolded tube lined by a single layer of columnar epithelial cells (future enterocytes). During the endogenous feeding period, comprised between 20 and 96 h post-fertilization (3.5 to 6.1 mm TL), the larval digestive system experienced a fast transformation with the almost complete development and differentiation of most of digestive organs (buccopahrynx, oesophagus, intestine, liver and exocrine pancreas). Yolk reserves were not completely depleted at the onset of exogenous feeding (4 dpf, 6.1 mm TL), and a period of mixed nutrition was observed up to 6 to 7 dpf (6.8 to 7.3 mm TL) when yolk was definitively exhausted. The stomach was the organ that latest achieved its complete differentiation, characterized by the development of abundant gastric glands in the fundic stomach between 10 and 15 dpf (10.9 to 15.8 mm TL) and the formation of the pyloric sphincter at the junction of the pyloric stomach and the anterior intestine at 15 dpf (15.8 mm TL). The above-mentioned morphological and histological features observed suggested the achievement of a digestive system characteristic of P. punctifer juveniles and adults. The ontogeny of the digestive system in P. punctifer followed the same general pattern as in most Siluriform species so far, although some species-specific differences in the timing of differentiation of several digestive structures were noted, which might be related to different reproductive guilds, egg and larval size or even different larval rearing practices. According to present findings on the histological development of the digestive system in P. punctifer, some recommendations regarding the rearing practices of this species are also provided in order to improve the actual larval rearing techniques of this fast-growing Neotropical catfish species.

}, issn = {1751-732X}, doi = {10.1017/S1751731114001797}, author = {Gisbert, E and Moreira, C and Castro-Ruiz, D and Ozt{\"u}rk, S and Fern{\'a}ndez, C and Gilles, S and Jesus Nu{\~n}ez-Rodriguez and Fabrice Duponchelle and Salvador Tello and Renno, J F and Garc{\'\i}a-D{\'a}vila, C and Darias, Maria} } @article {4108, title = {An integrated closed system for fish-plankton aquaculture in Amazonian fresh water.}, journal = {Animal}, volume = {8}, year = {2014}, month = {2014 Aug}, pages = {1319-28}, abstract = {

A prototype of an integrated closed system for fish-plankton aquaculture was developed in Iquitos (Peruvian Amazonia) in order to cultivate the Tiger Catfish, Pseudoplatystoma punctifer (Castelnau, 1855). This freshwater recirculating system consisted of two linked sewage tanks with an intensive rearing unit (a cage) for P. punctifer placed in the first, and with a fish-plankton trophic chain replacing the filters commonly used in clear water closed systems. Detritivorous and zooplanktivorous fishes (Loricariidae and Cichlidae), maintained without external feeding in the sewage volume, mineralized organic matter and permitted the stabilization of the phytoplankton biomass. Water exchange and organic waste discharge were not necessary. In this paper we describe the processes undertaken to equilibrate this ecosystem: first the elimination of an un-adapted spiny alga, Golenkinia sp., whose proliferation was favored by the presence of a small rotifer, Trichocerca sp., and second the control of this rotifer proliferation via the introduction of two cichlid species, Acaronia nassa Heckel, 1840 and Satanoperca jurupari Heckel, 1840, in the sewage part. This favored some development of the green algae Nannochloris sp. and Chlorella sp. At that time we took the opportunity to begin a 3-month rearing test of P. punctifer. The mean specific growth rate and feed conversion ratio (FCR) of P. punctifer were 1.43 and 1.27, respectively, and the global FCR, including fish in the sewage part, was 1.08. This system has proven to be suitable for growing P. punctifer juveniles out to adult, and provides several practical advantages compared with traditional recirculating clear water systems, which use a combination of mechanical and biological filters and require periodic waste removal, leading to water and organic matter losses.

}, issn = {1751-732X}, doi = {10.1017/S1751731114001165}, author = {Gilles, S and Ismi{\~n}o, R and S{\'a}nchez, H and Frank David and Jesus Nu{\~n}ez-Rodriguez and Dugu{\'e}, R and Darias, Maria and R{\"o}mer, U} }