@article {6883, title = {Lipidome variations of deep-sea vent shrimps according to acclimation pressure: A homeoviscous response?}, journal = {Deep Sea Research Part I: Oceanographic Research Papers}, year = {2020}, month = {Jan-04-2020}, pages = {103285}, issn = {09670637}, doi = {10.1016/j.dsr.2020.103285}, url = {https://linkinghub.elsevier.com/retrieve/pii/S096706372030073X}, author = {Bruce Shillito and Desurmont, C. and Barthelemy, D. and Farabos, D. and Despr{\'e}s, G. and Juliette Ravaux and Zbinden, M. and Lamazi{\`e}re, A.} } @article {6879, title = {Are shallow-water shrimps proxies for hydrothermal-vent shrimps to assess the impact of deep-sea mining?}, journal = {Marine Environmental Research}, volume = {151}, year = {2019}, month = {Jan-10-2019}, pages = {104771}, issn = {01411136}, doi = {10.1016/j.marenvres.2019.104771}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0141113619303216}, author = {Mestre, N.C. and Auguste, M. and de S{\'a}, L.C. and Fonseca, T.G. and Cardoso, C. and Brown, A. and Barthelemy, D. and Charlemagne, N. and Hauton, C. and Machon, J. and Juliette Ravaux and Bruce Shillito and Thatje, S. and Bebianno, M.J.} } @article {6876, title = {Assessing a species thermal tolerance through a multiparameter approach: the case study of the deep-sea hydrothermal vent shrimp Rimicaris exoculata}, journal = {Cell Stress and Chaperones}, volume = {24}, year = {2019}, month = {Jan-05-2019}, pages = {647 - 659}, issn = {1355-8145}, doi = {10.1007/s12192-019-01003-0}, url = {http://link.springer.com/10.1007/s12192-019-01003-0}, author = {Juliette Ravaux and L{\'e}ger, Nelly and Hamel, G{\'e}rard and Bruce Shillito} } @article {6887, title = {High rates of apoptosis visualized in the symbiont-bearing gills of deep-sea Bathymodiolus mussels}, journal = {PLOS ONE}, volume = {14}, year = {2019}, month = {Apr-02-2019}, pages = {e0211499}, doi = {10.1371/journal.pone.021149910.1371}, url = {http://dx.plos.org/10.1371/journal.pone.0211499}, author = {Piquet, B{\'e}r{\'e}nice and Bruce Shillito and Lallier, Fran{\c c}ois H. and Duperron, S{\'e}bastien and Andersen, Ann C.}, editor = {Rodrigues, Clara F.} } @article {6888, title = {Is It First the Egg or the Shrimp? {\textendash} Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development}, journal = {Frontiers in Microbiology}, volume = {10}, year = {2019}, month = {May-04-2020}, doi = {10.3389/fmicb.2019.00808}, url = {https://www.frontiersin.org/article/10.3389/fmicb.2019.00808/full}, author = {Methou, Pierre and Hern{\'a}ndez-{\'A}vila, Ivan and Aube, Johanne and Cueff-Gauchard, Val{\'e}rie and Gayet, Nicolas and Amand, Louis and Bruce Shillito and Pradillon, Florence and Cambon-Bonavita, Marie-Anne} } @article {6884, title = {Protein expression profiles in Bathymodiolus azoricus exposed to cadmium}, journal = {Ecotoxicology and Environmental Safety}, volume = {171}, year = {2019}, month = {Jan-04-2019}, pages = {621 - 630}, issn = {01476513}, doi = {10.1016/j.ecoenv.2019.01.031}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0147651319300399}, author = {Company, Rui and Ant{\'u}nez, Oreto and Cosson, Richard P. and Serafim, Angela and Bruce Shillito and Cajaraville, Miren and Bebianno, Maria Jo{\~a}o and Torreblanca, Amparo} } @article {4931, title = {Comparative Study of Chemosensory Organs of Shrimp From Hydrothermal Vent and Coastal Environments}, journal = {Chemical Senses}, volume = {doi:10.1093/chemse/bjx007}, year = {2017}, pages = {1-13}, abstract = {

The detection of chemical signals is involved in a variety of crustacean behaviors, such as social
interactions, search and evaluation of food and navigation in the environment. At hydrothermal
vents, endemic shrimp may use the chemical signature of vent fluids to locate active edifices,
however little is known on their sensory perception in these remote deep-sea habitats. Here,
we present the first comparative description of the sensilla on the antennules and antennae
of 4 hydrothermal vent shrimp (Rimicaris exoculata, Mirocaris fortunata, Chorocaris chacei,
and Alvinocaris markensis) and of a closely related coastal shrimp (Palaemon elegans). These
observations revealed no specific adaptation regarding the size or number of aesthetascs
(specialized unimodal olfactory sensilla) between hydrothermal and coastal species. We also
identified partial sequences of the ionotropic receptor IR25a, a co-receptor putatively involved in
olfaction, in 3 coastal and 4 hydrothermal shrimp species, and showed that it is mainly expressed
in the lateral flagella of the antennules that bear the unimodal chemosensilla aesthetascs.

}, keywords = {aesthetascs, decapod, hydrothermal shrimp, IR25a, olfaction}, author = {Magali Zbinden and Berthod, C and Montagn{\'e}, N and Machon, J and L{\'e}ger, N and Chertemps, T and Rabet, N and Bruce Shillito and Juliette Ravaux} } @article {6820, title = {Identifying Toxic Impacts of Metals Potentially Released during Deep-Sea Mining{\textemdash}A Synthesis of the Challenges to Quantifying Risk}, journal = {Frontiers in Marine Science}, volume = {4}, year = {2017}, pages = {368}, abstract = {In January 2017, the International Seabed Authority released a discussion paper on the development of Environmental Regulations for deep-sea mining (DSM) within the Area Beyond National Jurisdiction (the {\textquotedblleft}Area{\textquotedblright}). With the release of this paper, the prospect for commercial mining in the Area within the next decade has become very real. Moreover, within nations{\textquoteright} Exclusive Economic Zones, the exploitation of deep-sea mineral ore resources could take place on very much shorter time scales and, indeed, may have already started. However, potentially toxic metal mixtures may be released at sea during different stages of the mining process and in different physical phases (dissolved or particulate). As toxicants, metals can disrupt organism physiology and performance, and therefore may impact whole populations, leading to ecosystem scale effects. A challenge to the prediction of toxicity is that deep-sea ore deposits include complex mixtures of minerals, including potentially toxic metals such as copper, cadmium, zinc, and lead, as well as rare earth elements. Whereas the individual toxicity of some of these dissolved metals has been established in laboratory studies, the complex and variable mineral composition of seabed resources makes the a priori prediction of the toxic risk of DSM extremely challenging. Furthermore, although extensive data quantify the toxicity of metals in solution in shallow-water organisms, these may not be representative of the toxicity in deep-sea organisms, which may differ biochemically and physiologically and which will experience those toxicants under conditions of low temperature, high hydrostatic pressure, and potentially altered pH. In this synthesis, we present a summation of recent advances in our understanding of the potential toxic impacts of metal exposure to deep-sea meio- to megafauna at low temperature and high pressure, and consider the limitation of deriving lethal limits based on the paradigm of exposure to single metals in solution. We consider the potential for long-term and far-field impacts to key benthic invertebrates, including the very real prospect of sub-lethal impacts and behavioral perturbation of exposed species. In conclusion, we advocate the adoption of an existing practical framework for characterizing bulk resource toxicity in advance of exploitation.}, issn = {2296-7745}, doi = {10.3389/fmars.2017.00368}, url = {https://www.frontiersin.org/article/10.3389/fmars.2017.00368}, author = {Hauton, Chris and Brown, Alastair and Thatje, Sven and Mestre, N{\'e}lia C. and Bebianno, Maria J. and Martins, In{\^e}s and Bettencourt, Raul and Canals, Miquel and Sanchez-Vidal, Anna and Bruce Shillito and Juliette Ravaux and Magali Zbinden and Duperron, S{\'e}bastien and Mevenkamp, Lisa and Vanreusel, Ann and Gambi, Cristina and Dell{\textquoteright}Anno, Antonio and Danovaro, Roberto and Gunn, Vikki and Weaver, Phil} } @article {5740, title = {Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata}, journal = {Aquatic Biology}, volume = {175}, year = {2016}, pages = {277-285}, doi = {https://doi.org/10.1016/j.aquatox.2016.03.024}, author = {Auguste, M and Mestre, N and Rocha, T and Cardoso, C and Cueff-Gauchard, V and Le Bloa, S and Cambon-Bonavita, M-A and Bruce Shillito and Magali Zbinden and Juliette Ravaux and Bebianno, M} } @article {6896, title = {Estimating Symbiont Abundances and Gill Surface Areas in Specimens of the Hydrothermal Vent Mussel Bathymodiolus puteoserpentis Maintained in Pressure Vessels}, journal = {Frontiers in Marine Science}, volume = {3}, year = {2016}, month = {Apr-02-2017}, doi = {10.3389/fmars.2016.00016}, url = {http://journal.frontiersin.org/Article/10.3389/fmars.2016.00016/abstract}, author = {Duperron, S{\'e}bastien and Quiles, Adrien and Szafranski, Kamil M. and L{\'e}ger, Nelly and Bruce Shillito} } @article {6823, title = {Plasticity and acquisition of the thermal tolerance (upper thermal limit and heat shock response) in the intertidal species Palaemon elegans}, journal = {Journal of Experimental Marine Biology and Ecology}, volume = {484}, year = {2016}, pages = {39 - 45}, abstract = {The marine species sensitivity to climate change will depend on the ways by which these species can adapt to thermal increase and heterogeneity. Here, we present evidence that the intertidal shrimp Palaemon elegans acclimates its thermal tolerance, in response to environmental water temperature, through a significant shift of its upper thermal limit with no concomittant acclimation of the heat shock response (hsp70 stress gene expression threshold). This species is less thermotolerant than its congener Palaemonetes varians, and would therefore potentially be more sensitive to an increase in environmental temperature, such as imposed by global warming. In P. elegans life cycle, physiological adjustments like the shift of the thermal limit and the acquisition of a significant HSR, occurred during the metamorphosis from larvae to post-larvae. This suggests that this step is a genetically-programmed milestone in the process of thermal tolerance acquisition.}, keywords = {acclimation, Caridea, Development, heat stress, hsp70, thermal biology}, issn = {0022-0981}, doi = {https://doi.org/10.1016/j.jembe.2016.07.003}, url = {http://www.sciencedirect.com/science/article/pii/S0022098116301125}, author = {Juliette Ravaux and L{\'e}ger, Nelly and Rabet, Nicolas and Fourgous, Claire and Voland, Guillaume and Magali Zbinden and Bruce Shillito} } @article {3747, title = {Acute combined pressure and temperature exposures on a shallow-water crustacean: novel insights into the stress response and high pressure neurological syndrome.}, journal = {Comp Biochem Physiol A Mol Integr Physiol}, volume = {181}, year = {2015}, month = {2015 Mar}, pages = {9-17}, abstract = {

Little is known about the ecological and physiological processes governing depth distribution limits in species. Temperature and hydrostatic pressure are considered to be two dominant factors. Research has shown that some marine ectotherms are shifting their bathymetric distributions in response to rapid anthropogenic ocean surface warming. Shallow-water species unable to undergo latitudinal range shifts may depend on bathymetric range shifts to seek refuge from warming surface waters. As a first step in constraining the molecular basis of pressure tolerance in shallow water crustaceans, we examined differential gene expression in response to acute pressure and temperature exposures in juveniles of the shallow-water shrimp Palaemonetes varians. Significant increases in the transcription of genes coding for an NMDA receptor-regulated protein, an ADP ribosylation factor, β-actin, two heat shock protein 70 kDa isoforms (HSP70), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were found in response to elevated pressure. NMDA receptors have been implicated in pathways of excitotoxic damage to neurons and the onset of high pressure neurological syndrome (HPNS) in mammals. These data indicate that the sub-lethal effects of acute barotrauma are associated with transcriptional disturbances within the nervous tissue of crustaceans, and cellular macromolecular damage. Such transcriptional changes lead to the onset of symptoms similar to that described as HPNS in mammals, and may act as a limit to shallow water organisms{\textquoteright} prolonged survival at depth.

}, issn = {1531-4332}, doi = {10.1016/j.cbpa.2014.10.028}, author = {Morris, J P and Thatje, S and Juliette Ravaux and Bruce Shillito and Fernando, D and Hauton, C} } @article {6824, title = {Behavioural study of two hydrothermal crustacean decapods: Mirocaris fortunata and Segonzacia mesatlantica, from the Lucky Strike vent field (Mid-Atlantic Ridge)}, journal = {Deep Sea Research Part II: Topical Studies in Oceanography}, volume = {121}, year = {2015}, pages = {146 - 158}, abstract = {Identifying the factors driving community dynamics in hydrothermal vent communities, and in particular biological interactions, is challenged by our ability to make direct observations and the difficulty to conduct experiments in those remote ecosystems. As a result, we have very limited knowledge on species׳ behaviour and interactions in these communities and how they in turn influence community dynamics. Interactions such as competition or predation significantly affect community structure in vent communities, and video time-series have successfully been used to gain insights in biological interactions and species behaviour, including responses to short-term changes in temperature or feeding strategies. In this study, we combined in situ and ex situ approaches to characterise the behaviour and interactions among two key species encountered along the Mid-Atlantic Ridge (MAR): the shrimp Mirocaris fortunata and the crab Segonzacia mesatlantica. In situ, species small-scale distribution, interactions and behaviour were studied using the TEMPO observatory module deployed on the seafloor at the base of the active Eiffel Tower edifice in the Lucky Strike vent field as part of the EMSO-A{\c c}ores MoMAR observatory. TEMPO sampled 2min of video four times a day from July 2011 to April 2012. One week of observations per month was used for {\textquoteleft}long-term{\textquoteright} variations, and a full video data set was analysed for January 2012. In addition, observations of crab and shrimp individuals maintained for the first time under controlled conditions in atmospheric pressure (classic tank) and pressurised (AbyssBox) aquaria allowed better characterisation and description of the different types of behaviour and interactions observed in nature. While the identified in situ spatial distribution pattern was stable over the nine months, both species displayed a significant preference for mussel bed and anhydrite substrata, and preferentially occupied the area located directly in the fluid flow axis. The aggregation behaviour of M. fortunata resulted in the occurrence of numerous intraspecific interactions mainly involving the use of two pairs of sensory organs (antenna/antennule) and fleeing behaviours when in contact or close to individuals of S. mesatlantica. The higher level of passiveness observed in the ex situ artificial environment compared to the in situ environment was attributed to the lack of stimulation related to low densities of congeners and/or of sympatric species compared to the natural environment and the absence of continuous food supply, as both species displayed a significant higher level of activity during feeding time. This result emphasises the role of food supply as a driver of species distribution and behaviour. Direct in situ observations using cameras deployed on deep-sea observatories, combined with experimental set-up in pressurised aquaria, will help investigators understand the factors influencing community dynamics and species biology at vents as well as their underlying mechanisms.}, keywords = {32{\textdegree}16.3'W, 37{\textdegree}17'N, AbyssBox, Biological interactions, Deep-sea observatory, Eiffel Tower edifice, Experimental research, Feeding behaviour, Lucky Strike, Mid-Atlantic Ridge, Time series, Video imagery}, issn = {0967-0645}, doi = {https://doi.org/10.1016/j.dsr2.2015.04.008}, url = {http://www.sciencedirect.com/science/article/pii/S0967064515001113}, author = {Matabos Marjolaine and Cuvelier Daphn{\'e} and Brouard Johan and Bruce Shillito and Juliette Ravaux and Magali Zbinden and Barthelemy Dominique and Sarradin, Pierre-Marie and Sarrazin, Jozee} } @article {6878, title = {Characterising multi-level effects of acute pressure exposure on a shallow-water invertebrate: insights into the kinetics and hierarchy of the stress response}, journal = {Journal of Experimental Biology}, volume = {218}, year = {2015}, month = {Jan-08-2015}, pages = {2594 - 2602}, issn = {0022-0949}, doi = {10.1242/jeb.125914}, url = {http://jeb.biologists.org/cgi/doi/10.1242/jeb.125914}, author = {Morris, J. P. and Thatje, S. and Juliette Ravaux and Bruce Shillito and Hauton, C.} } @article {3744, title = {Is the deep-sea crab Chaceon affinis able to induce a thermal stress response?}, journal = {Comp Biochem Physiol A Mol Integr Physiol}, volume = {181}, year = {2015}, month = {2015 Mar}, pages = {54-61}, abstract = {

Fluctuations in the stress level of an organism are expressed in behavioural and molecular changes that can affect its ecology and survival. Our knowledge of thermal adaptations in deep-sea organisms is very limited, and this study investigates the critical thermal maximum (CTmax) and the heat-shock response (HSR) in the deep-sea crab Chaceon affinis commonly found in waters of the North East Atlantic. A mild but significant HSR in C. affinis was noted and one of the lowest CTmax known amongst Crustacea was revealed (27.5 {\textdegree}C at 0.1 MPa; 28.5 {\textdegree}C at 10 MPa). The thermal sensitivity of this species appears to be reduced at in situ pressure (10 MPa), given the slightly higher CTmax and the significant 3-fold induction of stress genes hsp70 form 1 and hsp70 form 2. Although C. affinis deep-sea habitat is characterized by overall low temperature this species appears to have retained its ability to induce a HSR. This capability may be linked with C. affinis{\textquoteright} occasional exploitation of warmer and thermally instable hydrothermal vent fields, where it has been found foraging for food.

}, issn = {1531-4332}, doi = {10.1016/j.cbpa.2014.11.015}, author = {Mestre, N{\'e}lia C and Cottin, Delphine and Bettencourt, Raul and Cola{\c c}o, Ana and Correia, S{\'e}rgio P C and Bruce Shillito and Thatje, Sven and Juliette Ravaux} } @article {9056, title = {Long-term maintenance and public exhibition of deep-sea hydrothermal fauna: The AbyssBox project}, journal = {Deep Sea Research Part II: Topical Studies in Oceanography}, volume = {121}, year = {2015}, month = {Jan-11-2015}, pages = {137 - 145}, issn = {09670645}, doi = {10.1016/j.dsr2.2015.05.002}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0967064515001460}, author = {Bruce Shillito and Juliette Ravaux and Sarrazin, Jozee and Zbinden, M. and Sarradin, Pierre-Marie and Barthelemy, D.} } @article {6825, title = {Long-term maintenance and public exhibition of deep-sea hydrothermal fauna: The AbyssBox project}, journal = {Deep Sea Research Part II: Topical Studies in Oceanography}, volume = {121}, year = {2015}, pages = {137 - 145}, abstract = {The AbyssBox project aims to provide the first permanent public exhibition of live deep-sea hydrothermal fauna maintained at in situ pressure. AbyssBox is a pressurized aquarium designed to function permanently. Here we present details of the project after the public exhibition functioned for more than three years at Oc{\'e}anopolis aquarium in Brest, France. We also describe the AbyssBox pressure aquarium, and provide data and observations on vent shrimp (Mirocaris fortunata) and crabs (Segonzacia mesatlantica) that were sampled from 1700m depth at the Lucky Strike vent field (Mid-Atlantic Ridge) during different cruises. While mortalities exceeded 50\% during the first days following sampling, the remaining animals appeared to acclimate fairly well. Some crabs have now been kept for more than 2 years, and some shrimp have spent more than 3 years in captivity. Primarily designed for a public exhibition, the AbyssBox is already used for scientific purposes, since it provides one of the most effective tools for long-term rearing of deep-sea fauna. AbyssBox is a first step towards maintaining a variety of deep-sea fauna year-round at in situ pressure, which will serve both scientific and public interests.}, keywords = {AbyssBox, Aquaria, Aquariology, Deep waters, Hydrostatic Pressure, Hydrothermal Vents, Public exhibition, Scientific experiments}, issn = {0967-0645}, doi = {https://doi.org/10.1016/j.dsr2.2015.05.002}, url = {http://www.sciencedirect.com/science/article/pii/S0967064515001460}, author = {Bruce Shillito and Juliette Ravaux and Sarrazin, Jozee and Magali Zbinden and Sarradin, Pierre-Marie and Barthelemy Dominique} } @article {6880, title = {The potential for climate-driven bathymetric range shifts: sustained temperature and pressure exposures on a marine ectotherm, Palaemonetes varians}, journal = {Royal Society Open Science}, volume = {2}, year = {2015}, month = {Jan-11-2015}, pages = {150472}, issn = {2054-5703}, doi = {10.1098/rsos.150472}, url = {https://royalsocietypublishing.org/doi/10.1098/rsos.150472}, author = {Morris, J. P. and Thatje, S. and Cottin, D. and Oliphant, A. and Brown, A. and Bruce Shillito and Juliette Ravaux and Hauton, C.} } @article {6881, title = {Relative abundances of methane- and sulfur-oxidizing symbionts in gills of the deep-sea hydrothermal vent mussel Bathymodiolus azoricus under pressure}, journal = {Deep Sea Research Part I: Oceanographic Research Papers}, volume = {101}, year = {2015}, month = {Jan-07-2015}, pages = {7 - 13}, issn = {09670637}, doi = {10.1016/j.dsr.2015.03.003}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0967063715000576}, author = {Szafranski, Kamil M. and Piquet, B{\'e}r{\'e}nice and Bruce Shillito and Lallier, Fran{\c c}ois H. and Duperron, S{\'e}bastien} } @article {3760, title = {The IPOCAMP pressure incubator for deep-sea fauna}, journal = {J Mar Sci Technol}, volume = {22}, year = {2014}, pages = {97-102}, doi = {10.6119/JMST-013-0718-3 }, url = {https://pdfs.semanticscholar.org/0804/23beebe022c1f0a644738e3ade83632322e8.pdf}, author = {Bruce Shillito and Fran{\c c}oise Gaill and Juliette Ravaux} }