%0 Journal Article %J General and Comparative Endocrinology %D 2003 %T Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor %A Gricourt, L %A Bonnec, G %A Boujard, D %A Mathieu, M %A Kristell Kellner-Cousin %X The involvement of molecules belonging to the insulin/IGF family in regulation of growth has been investigated in the Pacific oyster Crassostrea gigas. In vitro biological effects of human recombinant IGF-1 (hrIGF-1) on mantle edge cells, involved in oyster shell and soft body growth, were studied over an annual cycle. In mantle edge cells hrIGF-1 stimulates protein synthesis of 56 ± 5.1% over basal for 10−10 M in September with in addition a clear dose-effect corresponding to the highest shell growth period, and 57.5 ± 3.45% over basal for 10−11 M in March and 51 ± 5.4% over basal for 10−10 M in April corresponding to the period of mantle growth. These insulin-like effects were associated with the expression of a recently identified C. gigas insulin receptor-related receptor (CIR) in mantle edge cells as demonstrated by RT-PCR. Moreover, in situ hybridisation (ISH) confirmed this expression at the level of the inner and outer epithelia involved in mantle growth and shell formation. %B General and Comparative Endocrinology %V 134 %P 44–56 %G eng