@article {Auzoux-Bordenave2005,
	title = {{Comparative effects of a non-steroidal ecdysone agonist RH-5992 and 20-hydroxyeedysone in a lepidopteran cell line (IAL-PID2)}},
	journal = {Insect Biochemistry and Molecular Biology},
	volume = {35},
	year = {2005},
	pages = {1033{\textendash}1042},
	abstract = {The non-steroidal ecdysone agonist, RH-5992, exhibits ecdysteroid activities in vivo as well as in vitro more effectively than 20-hydroxyecdysone (20E). Using the IAL-PID2 cells derived from imaginal wing discs of last larval instar of Plodia interpunctella, we investigated the action of RH-5992 in the control of cell growth. Its effects on the proliferative activity of IAL-PID2 cells, the induction level in G2/M arrest and on the expression rate of Plodia B cyclin (PcycB), ecdysone B1-isoform (PIEcR-B1) and Ultraspiracle-2 isoform (PIUSP-2) were examined. From these cellular and molecular assays, our results brought evidence that RH-5992, like 20E, induced an inhibition oil cell proliferation by blocking IAL-PID2 cells in G2/M phase. Moreover, this G2/M arrest was preceded by a decrease in the expression level of PcycB and a high induction of PIEcR-B1, PIUSP-2 mRNAs. Dose-response experiments revealed that RH-5992 was even more potent than 20E. On these parameters, we therefore suggest that the differential observed in the expression level of USP and EcR by RH-5992 and 20E could contribute to the difference observed for the biological potency of these two compounds. (C) 2005 Elsevier Ltd. All rights reserved.},
	issn = {0965-1748},
	doi = {10.1016/j.ibmb.2005.04.004},
	author = {St{\'e}phanie Auzoux-Bordenave and Solvar, M and Queguiner, I and Bozzolan, F and Mottier, V and Siaussat, D and Porcheron, P and Debernard, S}
}
