@article {Fabioux_etal2009,
	title = {{<i>In vivo</i> RNA interference in oyster : <i>vasa</i> silencing inhibits germ cell development}},
	journal = {FEBS Journal},
	volume = {276},
	number = {9},
	year = {2009},
	pages = {2566{\textendash}2573},
	publisher = {Blackwell Publishing},
	abstract = {This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea\{\^A}\}\~{}gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In\{\^A}\}\~{}vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39\% and 87\%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea\{\^A}\}\~{}gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in\{\^A}\}\~{}vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies.},
	issn = {1742-4658},
	doi = {equipe1},
	author = {C Fabioux and Corporeau, C and Virgile Quillien and Pascal Favrel and Huvet, A}
}
