@article {Rousseau2006,
	title = {{Restoration of stratum corneum with nacre lipids}},
	journal = {Comparative Biochemistry and Physiology B-Biochemistry \& Molecular Biology},
	volume = {145},
	year = {2006},
	pages = {1{\textendash}9},
	abstract = {To discover potential new products for the atopic dermatitis treatment, lipids extracted from nacre from the oyster Pinctada margaritifera were tested on artificially dehydrated skin explants. Expression of filaggrin and transglutaminase I was investigated after treatment of dehydrated skin with P margaritifera lipid extracts according to light microscopy after labelling with specific monoclonal antibodies. The lipids were extracted from the nacre with methanol/chloroform mixture at room temperature and the extract composition was determined according to TLC and densitometry measures. Relative to the dry nacre material, a yield of extraction in lipids of 0.54\% (w/w) was determined. Fatty acids, triglycerides, cholesterol and ceramides were in low abundance. Then, application of lipid formulations on skin explants previously dehydrated gave after 3 h an overexpression of filaggrin and a decrease of transglutaminase expression as shown by light microscopy. Using immunofluorescence labelling, we showed that lipids extracted from the mother of pearl of P. margaritifera induced a reconstitution of the intercellular cement of the stratum corneum. The signaling properties of the nacre lipids could be used for a development of new active product treatment against the symptoms of the dermatitis. (c) 2006 Elsevier Inc. All rights reserved.},
	issn = {1096-4959},
	doi = {10.1016/j.cbpb.2006.06.012},
	author = {Rousseau, M and Bedouet, L and Lati, E and Gasser, P and Le Ny, K and Lopez, E}
}
