@article {3641,
	title = {Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France).},
	journal = {Ecotoxicol Environ Saf},
	volume = {97},
	year = {2013},
	month = {2013 Nov},
	pages = {103-13},
	abstract = {<p>Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Among those, the Loire estuary (France) is potentially exposed to various contaminants including Endocrine Disruptors Compounds (EDCs) able to impact the reproduction physiology of fish. The European eel (Anguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor the effects of endocrine disruption. Despite this weakly responsiveness, this study aimed to investigate whether European eel from the Loire estuary may still be the subject of estrogenic disruption quantifying the hepatic Vg gene expression according to gender and sexual stage. Vitellogenin (Vg) appears as a valuable biomarker of EDCs, as well as for exposure and effects. Quantitative real-time Reverse Transcription Polymerase Chain Reaction (q RT PCR) was used in this study to amplify responses of hepatic Vg transcripts. European eels were sampled in May 2009 (N=57) and November 2010 (during the downstream migration, N=10) in two sites of the Loire estuary with different ecological conditions and contamination pressures (upstream: Varades; downstream: Nantes). Reproductive (gender, sexual maturity stage) and biometric parameters of collected eels were determined. A laboratory exposure of silver male to steroid hormones (Testosterone (T), 11-KetoTestosterone (11-KT), Estradiol (E2)) was conducted in parallel to validate the q RT PCR approach on hepatic Vg mRNA. Results demonstrated the responsiveness of exposed silver male eels, since hepatic mRNA Vg induction was observed in E2 treated males compared to control specimens. In the field, results of female silver eels reflected large inter-individual differences in the activation of hepatic Vg at silvering. However, while only female silver eels should express hepatic Vg mRNA, quantifiable levels were also detected in a proportion of 38\% of the other individuals sampled, normally not inclined to express it, those being undifferentiated eels, yellow females, yellow and silver males. According to each sexual stage, no difference of expression was observed between eels from the two sampling sites. Histological results as well as low Vg mRNA levels detected do not permit a conclusion as to a potential effect of endocrine disruption.</p>
},
	keywords = {Anguilla, Animals, Body Size, Endangered Species, Endocrine Disruptors, Estuaries, Female, France, Gene Expression Regulation, Gonadal Steroid Hormones, Liver, Male, RNA, Messenger, Sexual Maturation, Vitellogenins, Water Pollutants, Chemical},
	issn = {1090-2414},
	doi = {10.1016/j.ecoenv.2013.07.019},
	author = {Blanchet-Letrouv{\'e}, Isabelle and Anne-Gaelle Lafont and Poirier, Laurence and Sylvie Baloche and Zalouk-Vergnoux, Aurore and Sylvie Dufour and Mouneyrac, Catherine}
}
