@article {4119,
	title = {Imbalanced dietary ascorbic acid alters molecular pathways involved in skeletogenesis of developing European sea bass (Dicentrarchus labrax).},
	journal = {Comp Biochem Physiol A Mol Integr Physiol},
	volume = {159},
	year = {2011},
	month = {2011 May},
	pages = {46-55},
	abstract = {<p>The influence of dietary ascorbic acid (AA) on growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45days post hatching. Diets incorporated 0, 5, 15, 30, 50 or 400mg AA per kg diet to give AA-0, AA-5, AA-15, AA-30, AA-50 and AA-400 dietary treatments, respectively. Dietary AA levels lower than 15mg/kg reduced larval growth and survival was affected in specimens fed diets devoid of AA. Globally, disruption of the expression of genes involved in AA and calcium absorption in the intestine (SVCT-1, TRPV-6), skeletogenesis (BMP-4, IGF-1, RARγ) and bone mineralization (VDRβ, osteocalcin) were observed in groups fed doses lower and higher than 50mg AA/kg diet. Such disturbances detected at molecular level were associated with disruptions of the ossification process and the appearance of skeletal abnormalities.</p>
},
	keywords = {Animals, Ascorbic Acid, Bass, Bone Morphogenetic Protein 4, Diet, Dose-Response Relationship, Drug, Fish Proteins, Gene Expression Regulation, Developmental, Insulin-Like Growth Factor I, Larva, Organic Anion Transporters, Sodium-Dependent, Osteocalcin, Osteogenesis, Receptors, Calcitriol, Receptors, Retinoic Acid, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, TRPV Cation Channels, Vitamins},
	issn = {1531-4332},
	doi = {10.1016/j.cbpa.2011.01.013},
	author = {Darias, Maria and Mazurais, D and Koumoundouros, Giorgo and Le Gall, Marie M and Huelvan, Christine and Desbruyeres, Elisabeth and Quazuguel, Patrick and Cahu, Chantal L and Zambonino-Infante, J}
}
