@article {9313, title = {Duplicated paralog of sulfide: quinone oxidoreductase contributes to the adaptation to hydrogen sulfide-rich environment in the hydrothermal vent crab, Xenograpsus testudinatus}, journal = {Science of The Total Environment}, volume = {890}, year = {2023}, month = {Jan-09-2023}, pages = {164257}, issn = {00489697}, doi = {10.1016/j.scitotenv.2023.164257}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0048969723028784}, author = {Chen, Chi and Wu, Guan-Chung and Chung, Yao-Tse and Li, Hau-Wen and Chou, Pei-Hsuan and Tseng, Yung-Che and Chen, Chieh-Jhen and Sylvie Dufour and Chang, Ching-Fong} } @article {8446, title = {Involvement of RFamide neuropeptides in polyp contraction of the adult scleractinian corals Euphyllia ancora and Stylophora pistillata}, journal = {General and Comparative Endocrinology}, volume = {314}, year = {2021}, month = {Jan-12-2021}, pages = {113905}, issn = {00166480}, doi = {10.1016/j.ygcen.2021.113905}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648021001982}, author = {Zhang, Yan and Shikina, Shinya and Ho, Yu-Ying and Chiu, Yi-Ling and I-Chen Yao, Jack and C{\'e}line Zatylny-Gaudin and Sylvie Dufour and Chang, Ching-Fong} } @article {8794, title = {Involvement of Transforming Growth Factor Beta family genes in gonadal differentiation in Japanese eel, Anguilla japonica, according to sex-related gene expressions}, journal = {Cells}, volume = {10}, year = {2021}, pages = {3007}, url = {https://doi.org/10.3390/cells10113007}, author = {Lin, Chien-Ju and Jeng, Shan-Ru and Lei, Zhen-Yuan and Yueh, When-Shiun and Sylvie Dufour and Wu, Guan-Chung and Chang, Ching-Fong} } @article {8085, title = {Molecular and cellular regulation on sex change in hermaphroditic fish, with a special focus on protandrous black porgy, Acanthopagrus schlegelii.}, journal = {Mol Cell Endocrinol}, volume = {520}, year = {2021}, month = {2021 01 15}, pages = {111069}, abstract = {

In teleost fish, sex can be determined by genetic factors, environmental factors, or both. Unlike in gonochoristic fish, in which sex is fixed in adults, sex can change in adults of hermaphroditic fish species. Thus, sex is generated during the initial gonadal differentiation stage (primary sex differentiation) and later during sexual fate alternation (secondary sex differentiation) in hermaphroditic fish species. Depending on the species, sex phase alternation can be induced by endogenous cues (such as individual age and body size) or by social cues (such as sex ratio or relative body size within the population). In general, the fluctuation in plasma estradiol-17β (E2) levels is correlated with the sexual fate alternation in hermaphroditic fish. Hormonal treatments can artificially induce sexual phase alternation in sequential hermaphroditic fishes, but in a transient and reversible manner. This is the case for the E2-induced female phase in protandrous black porgy and the methyltestosterone (MT)- or aromatase inhibitor (AI)-induced male phase in protogynous grouper. Recent reviews have focused on the different forms of sex change in fish who undergo sequential sex change, especially in terms of gene expression and the role of hormones. In this review, we use the protandrous black porgy, a nonsocial cue-influenced hermaphroditic species, with digonic gonads (ovarian and testis separated by a connective tissue), as a model to describe our findings and discuss the molecular and cellular regulation of sexual fate determination in hermaphroditic fish.

}, issn = {1872-8057}, doi = {10.1016/j.mce.2020.111069}, author = {Wu, Guan-Chung and Sylvie Dufour and Chang, Ching-Fong} } @article {7133, title = {Basal teleosts provide new insights into the evolutionary history of teleost-duplicated aromatase}, journal = {General and Comparative Endocrinology}, volume = {291}, year = {2020}, month = {Jan-05-2020}, pages = {113395}, issn = {00166480}, doi = {10.1016/j.ygcen.2020.113395}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648019303326}, author = {Lin, Chien-Ju and Maugars, Gersende and Lafont, Anne-Gaelle and Jeng, Shan-Ru and Wu, Guan-Chung and Sylvie Dufour and Chang, Ching-Fong} } @article {8081, title = {Elevated estradiol-17β levels inhibit final oocyte maturation via G protein-coupled estrogen receptor (Gper) in yellowfin porgy, Acanthopagrus latus}, journal = {General and Comparative Endocrinology}, volume = {299}, year = {2020}, month = {Jan-12-2020}, pages = {113587}, issn = {00166480}, doi = {10.1016/j.ygcen.2020.113587}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648020303403}, author = {Jeng, Shan-Ru and Thomas, Peter and Pang, Yefei and Sylvie Dufour and Lin, Chien-Ju and Yueh, Wen-Shiun and Chang, Ching-Fong} } @article {7023, title = {Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora}, journal = {Scientific Reports}, volume = {10}, year = {2020}, month = {Jan-12-2020}, abstract = {The existence and function of neurons remain largely unexplored in scleractinian corals. To gain a better understanding of neuronal functions in coral physiology, this study focused on Glycine-Leucine-Tryptophan-amide family neuropeptides (GLWamides), which have been shown to induce muscle contraction and larval metamorphosis in other cnidarians. Molecular identification and functional characterization of GLWamides in the adult stony coral\ Euphyllia ancora\ were performed. We successfully elucidated the full-length cDNA of GLWamide preprohormone in\ E. ancora\ (named EaGLW preprohormone). The deduced amino acid sequence was predicted to contain six potential GLWamide peptides. Tissue distribution analysis demonstrated that transcripts of\ EaGLW preprohormone\ were mainly expressed in the mouth (including the pharynx) and tentacles of the polyps. Immunodetection with an anti-GLWamide monoclonal antibody revealed that GLWamide neurons were mainly distributed in the epidermis of the mouth region and tentacle, in agreement with the distribution patterns of the transcripts. Treatment of the isolated mouth and tentacles with synthetic GLWamide peptides induced the contraction of these isolated tissues. Treatment of polyps with synthetic GLWamide peptides induced the contraction of polyps. These results suggest that GLWamides are involved in polyp contraction (myoactivity) in adult scleractinians. Our data provide new information on the physiological function of neuropeptides in scleractinians.}, doi = {10.1038/s41598-020-66438-3}, url = {http://www.nature.com/articles/s41598-020-66438-3}, author = {Shikina, Shinya and Chiu, Yi-Ling and Zhang, Yan and Yi-ChenYao and Liu, Tai-Yu and Tsai, Pin-Hsuan and C{\'e}line Zatylny-Gaudin and Chang, Ching-Fong} } @article {6017, title = {Activation of the brain-pituitary-gonadotropic axis in the black porgy Acanthopagrus schlegelii during gonadal differentiation and testis development and effect of estradiol treatment.}, journal = {General and Comparative Endocrinology}, volume = {281}, year = {2019}, pages = {17-29}, author = {Lin, Chien-Ju and Wu, Guan-Chung and Sylvie Dufour and Chang, Ching-Fong} } @article {6016, title = {Dmrt1 (doublesex and mab-3-related transcription factor 1) expression during gonadal development and spermatogenesis in the Japanese eel.}, journal = {General and Comparative Endocrinology}, volume = {279}, year = {2019}, pages = {154-163}, author = {Jeng, Shan-Ru and Wu, Guan-Chung and Wen-Shiun Yueh and Kuo, S-F and Sylvie Dufour and Chang, Ching-Fong} } @article {4506, title = {Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, Acanthopagrus schlegelii.}, journal = {Dev Neurobiol}, volume = {76}, year = {2016}, month = {2016 Feb}, pages = {121-36}, abstract = {

The early brain development, at the time of gonadal differentiation was investigated using a protandrous teleost, black porgy. This natural model of monosex juvenile fish avoids the potential complexity of sexual dimorphism. Brain neurogenesis was evaluated by histological analyses of the diencephalon, at the time of testicular differentiation (in fish between 90 and 150 days after hatching). Increases in the number of both Nissl-stained total brain cells, and Pcna-immunostained proliferative brain cells were observed in specific area of the diencephalon, such as ventromedialis thalami and posterior preoptic area, revealing brain cell proliferation. qPCR analyses showed significantly higher expression of the radial glial cell marker blbp and neuron marker bdnf. Strong immunohistochemical staining of Blbp and extended cellular projections were observed. A peak expression of aromatase (cyp19a1b), as well as an increase in estradiol (E2 ) content were also detected in the early brain. These data demonstrate that during gonadal differentiation, the early brain exhibits increased E2 synthesis, cell proliferation, and neurogenesis. To investigate the role of E2 in early brain, undifferentiated fish were treated with E2 or aromatase inhibitor (AI). E2 treatment upregulated brain cyp19a1b and blbp expression, and enhanced brain cell proliferation. Conversely, AI reduced brain cell proliferation. Castration experiment did not influence the brain gene expression patterns and the brain cell number. Our data clearly support E2 biosynthesis in the early brain, and that brain E2 induces neurogenesis. These peak activity patterns in the early brain occur at the time of gonad differentiation but are independent of the gonads.

}, issn = {1932-846X}, doi = {10.1002/dneu.22303}, author = {Lin, Chien-Ju and Fan-Chiang, Yi-Chun and Sylvie Dufour and Chang, Ching-Fong} } @article {5423, title = {Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, Acanthopagrus schlegelii.}, journal = {Developmental Neurobiology}, volume = {76}, year = {2016}, pages = {121-136}, doi = {DOI: 10.1002/dneu.22303}, author = {Lin, Chien-Ju and Fran-Chiang, Y C and Sylvie Dufour and Chang, Ching-Fong} } @article {3639, title = {Neuroendocrine gene expression reveals a decrease in dopamine D2B receptor with no changes in GnRH system during prepubertal metamorphosis of silvering in wild Japanese eel.}, journal = {Gen Comp Endocrinol}, volume = {206}, year = {2014}, month = {2014 Sep 15}, pages = {8-15}, abstract = {

Silvering is a prepubertal metamorphosis preparing the eel to the oceanic reproductive migration. A moderate gonad development occurs during this metamorphosis from the sedentary yellow stage to the migratory silver stage. The aim of this study was to elucidate the molecular aspects of various endocrine parameters of BPG axis at different ovarian developmental stages in wild yellow and silver female Japanese eels. The GSI of the sampled female eels ranged between 0.18 and 2.3\%, corresponding to yellow, pre-silver and silver stages. Gonad histology showed changes from previtellogenic oocytes in yellow eels to early vitellogenic oocytes in silver eels. Both serum E2 and T concentrations significantly increased with ovarian development indicating a significant activation of steroidogenesis during silvering. In agreement with previous studies, significant increases in pituitary gonadotropin beta subunits FSH-β and LH-β transcripts were also measured by qPCR, supporting that the activation of pituitary gonadotropin expression is likely responsible for the significant ovarian development observed during silvering. We investigated for the first time the possible brain neuroendocrine mechanisms involved in the activation of the pituitary gonadotropic function during silvering. By analyzing the expression of genes representative of the stimulatory GnRH control and the inhibitory dopaminergic control. The transcript levels of mGnRH and the three GnRH receptors did not change in the brain and pituitary between yellow and silver stages, suggesting that gene expression of the GnRH system is not significantly activated during silvering. The brain transcript levels of tyrosine hydroxylase, limiting enzyme of DA synthesis did not change during silvering, indicating that the DA synthesis activity was maintained. In contrast, a significant decrease in DA-D2B receptor expression in the forebrain and pituitary was observed, with no changes in DA-D2A receptor. The decrease in the pituitary expression of DA-D2BR during silvering would allow a reduced inhibitory effect of DA. We may raise the hypothesis that this regulation of D2BR gene expression is one of the neuroendocrine mechanisms involved in the slight activation of the pituitary gonadotropin and gonadal activity that occur at silvering.

}, keywords = {Animals, Cells, Cultured, Dopamine, Eels, Female, Follicle Stimulating Hormone, beta Subunit, Gene Expression Regulation, Gonadotropin-Releasing Hormone, Immunoblotting, Immunoenzyme Techniques, Luteinizing Hormone, beta Subunit, Metamorphosis, Biological, Neurosecretory Systems, Oocytes, Ovary, Real-Time Polymerase Chain Reaction, Receptors, Dopamine D2, Reproduction, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Sexual Maturation, Skin Pigmentation}, issn = {1095-6840}, doi = {10.1016/j.ygcen.2014.08.001}, author = {Jeng, Shan-Ru and Wen-Shiun Yueh and Pen, Yi-Ting and Lee, Yan-Horn and Chen, Guan-Ru and Sylvie Dufour and Chang, Ching-Fong} }