@article {3507,
	title = {Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata?},
	journal = {Environ Sci Pollut Res Int},
	year = {2015},
	month = {2014 Aug 19},
	abstract = {<p>In this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24\&nbsp;h to CdCl2 concentrations of 0, 1 and 100\&nbsp;μg\&nbsp;ml(-1). The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1\&nbsp;μg\&nbsp;ml(-1) of CdCl2.</p>
},
	issn = {1614-7499},
	doi = {10.1007/s11356-014-3387-5},
	author = {Ladhar-Chaabouni, R and Machreki-Ajmi, Monia and Antoine Serpentini and Jean-Marc Lebel and Hamza-Chaffai, A}
}
@article {4420,
	title = {The endocrine-disrupting effect and other physiological responses of municipal effluent on the clam Ruditapes decussatus.},
	journal = {Environ Sci Pollut Res Int},
	volume = {22},
	year = {2015},
	month = {2015 Dec},
	pages = {19716-28},
	abstract = {<p>In order to document the potential endocrine disrupting and toxic effect of the municipal wastewater effluents discharged into the Sfax coastal area (South of Tunisia), specimens of clam R. decussatus were collected from a reference site and were in vivo exposed to treated sewage effluent for 30 days. To this end, estrogenic and androgenic activities were measured in the gills to assess potential accumulation and regulation of active compounds. After effluent exposure androgenic activity in organic extracts increased up to fivefold compared to controls and remained elevated, while estrogenic activity was not significantly affected by exposure. As a consequence, remarkable disruptions in the gametogenesis activity, glycogen content, and Vitellogenin-like protein levels in male clams were observed. A parallel analysis of heavy metals in clam tissues was determined. A significant uptake of Ni, Zn, and Pb in soft tissues of exposed clams was observed. The significant increase of malondialdehyde (MDA) concentrations as a function of exposure time implies that clams have been exposed to an oxidative stress probably due to the presence of high metal concentrations in sewage effluent. Correlation analysis has revealed a statistically significant and positive relationship between MDA levels and metal concentrations in clams{\textquoteright} tissues. The acetylcholinesterase activity was not significantly affected by exposure. Altogether, these results showed that a short-term exposure to a mixture of chemical compounds released by the Sfax wastewater treatment plant induce adverse physiological and reproductive effects in R. decussatus. Further studies are underway in order to evaluate its long-term impacts on aquatic wildlife in the gulf of Gabes area.</p>
},
	issn = {1614-7499},
	doi = {10.1007/s11356-015-5199-7},
	author = {Mezghani-Chaari, Sawssan and Machreki-Ajmi, Monia and Tremolet, Gauthier and Kristell Kellner-Cousin and Geffard, Alain and Minier, Christophe and Hamza-Chaffai, Amel}
}
