@article {9558, title = {Ancestors{\textquoteright} Gift: Parental Early Exposure to the Environmentally Realistic Pesticide Mixture Drives Offspring Phenotype in a Larger Extent Than Direct Exposure in the Pacific Oyster, Crassostrea gigas}, journal = {Environmental Science \& Technology}, year = {2024}, issn = {0013-936X}, doi = {10.1021/acs.est.3c0820110}, url = {https://pubs.acs.org/doi/10.1021/acs.est.3c08201}, author = {Sol-Dourdin, Thomas and Guyomard, Killian and Rabiller, Manuella and Houssais, Nina and Cormier, Alexandre and Le Monier, Pauline and Sussarellu, Rossana and Guillaume Rivi{\`e}re} } @article {9560, title = {Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.}, journal = {Environmental Pollution}, volume = {326}, year = {2023}, month = {Jan-06-2023}, pages = {121472}, issn = {02697491}, doi = {10.1016/j.envpol.2023.121472}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0269749123004748}, author = {Sol-Dourdin, Thomas and Guillaume Rivi{\`e}re and Cormier, Alexandre and Di Poi, Carole and Guyomard, Killian and Rabiller, Manuella and Akcha, Farida and Bah Sadialiou, Thierno and Le Monier, Pauline and Sussarellu, Rossana} } @article {6469, title = {Realistic environmental exposure to microplastics does not induce biological effects in the Pacific oyster Crassostrea gigas}, journal = {Marine Pollution Bulletin}, volume = {150}, year = {2020}, month = {Jan-01-2020}, pages = {110627}, abstract = {The aim of the present study was to evaluate the presence and potential toxic effects of plastic fragments(\< 400μm) of polyethylene and polypropylene on the Pacific oysterCrassostrea gigas.Oysters were exposed toenvironmentally relevant concentrations (0, 0.008, 10, 100μg of particles/L) during 10 days, followed by adepuration period of 10 days in clean seawater. Effects of microplastics were evaluated on the clearance rate oforganisms, tissue alteration, antioxidant defense, immune alteration and DNA damage. Detection and quanti-fication of microplastics in oyster{\textquoteright}s tissues (digestive gland, gills and other tissues) and biodeposits using infraredmicroscopy were also conducted. Microplastics were detected in oyster{\textquoteright}s biodeposits following exposure to alltested concentrations: 0.003, 0.006 and 0.05 particles/mg of biodeposits in oysters exposed to 0.008, 10 and100μg of particles/L, respectively. No significant modulation of biological markers was measured in organismsexposed to microplastics in environmentally relevant conditions.}, keywords = {biodeposits, biomarkers, bivalves, Crassostrea gigas, microplastics}, issn = {0025326X}, doi = {10.1016/j.marpolbul.2019.110627}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X19307751}, author = {Revel, Messika and Ch{\^a}tel, Am{\'e}lie and Perrein-Ettajani, Hanane and Bruneau, M{\'e}lanie and Akcha, Farida and Sussarellu, Rossana and Rouxel, Julien and Katherine Costil and Decottignies, Priscilla and Cognie, Bruno and Lagarde, Fabienne and Mouneyrac, Catherine} } @article {6470, title = {Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations}, journal = {Frontiers in Environmental Science}, volume = {7}, year = {2019}, month = {Sep-03-2020}, abstract = {The impact of a microplastic (MP) mixture composed of polyethylene (PE) and polypropylene (PP) plastic particles, prepared from commercially available products, was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevant concentrations: 0.008 μg L-1 (low), 10 μg L-1 (medium), and 100 μg L-1 (high). Organisms were exposed for 10 days followed by 10 days of depuration in clean seawater under controlled laboratory conditions. The evaluation of MP effects on mussel clearance rate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNA integrity were investigated while the identification of plastic particles in mussel tissues (gills, digestive gland, and remaining tissues), and biodeposits (feces and pseudofaeces) was performed using infrared microscopy (μFT-IR). Results showed the presence of MPs only in the digestive gland of mussels exposed to the highest tested concentration of MPs with a mean of 0.75 particle/mussel (after the 10 days of exposure). In biodeposits, PE and PP particles were detected following exposure to all tested concentrations confirming the ingestion of MPs by the organisms. A differential response of antioxidant enzyme activities between digestive gland and gills was observed. Significant increases in superoxide dismutase (SOD) and catalase (CAT) activities were measured in the digestive gland of mussels exposed to the low (0.008 μg L-1) and medium (10 μg L-1) concentrations ofMPs and in the gills frommussels exposed to the highest concentration (100 μg L-1) of MPs that could be indicative of a change in the redox balance. Moreover, an increase in acid phosphatase activity was measured in hemolymph of mussels exposed to 0.008 and 10 μg L-1 concentrations. No significant difference was observed in the clearance rate, and histopathological parameters between control and exposed mussels. This study brings new insights on the potential sublethal impacts of MPs at environmentally relevant concentrations in marine bivalves.}, keywords = {biomarkers, microplastics, Mytilus, oxidative stress, polyethylene, polypropylene}, doi = {10.3389/fenvs.2019.00033}, url = {https://www.frontiersin.org/article/10.3389/fenvs.2019.00033}, author = {Revel, Messika and Lagarde, Fabienne and Perrein-Ettajani, Hanane and Bruneau, M{\'e}lanie and Akcha, Farida and Sussarellu, Rossana and Rouxel, Julien and Katherine Costil and Decottignies, Priscilla and Cognie, Bruno and Ch{\^a}tel, Am{\'e}lie and Mouneyrac, Catherine} } @article {5779, title = {Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos}, journal = {Aquat Toxicol}, volume = {196}, year = {2018}, pages = {70-78}, keywords = {Oyster Embryotoxicity Copper Gene expression DNA methylation}, doi = {doi: 10.1016/j.aquatox.2018.01.001}, url = {https://www.sciencedirect.com/science/article/pii/S0166445X18300018?via\%3Dihub}, author = {Sussarellu, Rossana and Lebreton Morgane and Rouxel Julien and Akcha Farida and Guillaume Rivi{\`e}re} } @article {4235, title = {Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas.}, journal = {BMC Genomics}, volume = {16}, year = {2015}, month = {2015}, pages = {808}, abstract = {

BACKGROUND: Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe.

METHODS: To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60).

RESULTS: A female-biased sex-ratio and a higher condition index were observed in the Danish progeny, possibly reflecting an evolutionary reproductive strategy to increase the potential success of natural recruitment in recently settled population. Using multifarious statistical approaches and accounting for sex differences we identified several transcripts differentially expressed between the Danish and French progenies, for which additive genetic basis is suspected (showing intermediate expression levels in hybrids, and therefore additivity). Candidate transcripts included mRNA coding for sperm quality and insulin metabolism, known to be implicated in coordinated control and success of reproduction.

CONCLUSIONS: Observed differences suggest that adaptation of invasive populations might have occurred during expansion acting on reproductive traits, and in particular on a female-biased sex-ratio, gamete quality and fertility.

}, issn = {1471-2164}, doi = {10.1186/s12864-015-1972-8}, author = {Sussarellu, Rossana and Huvet, Arnaud and Lap{\`e}gue, Sylvie and Virgile Quillien and Christophe Lelong and Cornette, Florence and Jensen, Lasse Fast and Bierne, Nicolas and Boudry, Pierre} }