@article {6116,
	title = {Characterization of an evolutionarily conserved calcitonin signalling system in a lophotrochozoan, the Pacific oyster ().},
	journal = {J Exp Biol},
	volume = {222},
	year = {2019},
	month = {2019 Jul 05},
	abstract = {<p>In Protostoma, the diuretic hormone 31 (DH31) signalling system was long considered as the orthologue of the chordate calcitonin (CT) signalling system. Using the Pacific oyster () transcriptomic database GigaTON, we characterized seven G-protein-coupled receptors (GPCRs) named Cragi-CTR1-7 and phylogenetically related to chordate CT receptors (CTRs) and to protostome DH31 receptors. Two CT precursors (Cragi-CTP1 and Cragi-CTP2) containing two CT-type peptides and encoded by two distinct genes with a similar organization were also characterized. These oyster neuropeptides (Cragi-CT1/2) exhibit the two N-terminal paired cysteine residues and, except CTP2-derived peptide (Cragi-CTP2dp), show the C-terminal proline-amide motif typical of deuterostome CT-type peptides. All mature Cragi-CTs except Cragi-CTP2dp were detected in visceral ganglion extracts using mass spectrometry. Cell-based assays revealed that the previously characterized oyster receptors Cg-CT-R and Cragi-CTR2 were specifically activated by Cragi-CT1b and Cragi-CT2, respectively. This activation does not require the co-expression of receptor activity-modifying proteins (RAMPs). Thus, oyster CT signalling appears functionally more closely related to vertebrate CT/CTR signalling than to calcitonin gene-related peptide/calcitonin receptor-like receptor (CGRP/CLR) signalling. Gene expression profiles in different adult tissues and in oysters acclimated to brackish water suggest the potential implication of both Cg-CT-R/Cragi-CT1b and Cragi-CTR2/Cragi-CT2 in water and ionic regulations, although with apparently opposite effects. The present study represents the first comprehensive characterization of a functional CT-type signalling system in a protostome and provides evidence for its evolutionarily ancient origin and its early role in osmotic homeostasis.</p>
},
	issn = {1477-9145},
	doi = {10.1242/jeb.201319},
	author = {Julie Schwartz and R{\'e}alis-Doyelle, Emilie and Marie-Pierre Dubos and Lefranc, Benjamin and Leprince, J{\'e}r{\^o}me and Pascal Favrel}
}
@article {6796,
	title = {Characterization of an evolutionarily conserved calcitonin signalling system in a lophotrochozoan, the Pacific oyster (Crassostrea gigas)      },
	journal = {The Journal of Experimental Biology},
	volume = {222},
	year = {2019},
	month = {Jan-07-2019},
	pages = {jeb201319},
	issn = {0022-0949},
	doi = {10.1242/jeb.201319},
	url = {http://jeb.biologists.org/lookup/doi/10.1242/jeb.201319},
	author = {Julie Schwartz and R{\'e}alis-Doyelle, Emilie and Marie-Pierre Dubos and Lefranc, Benjamin and Leprince, J{\'e}r{\^o}me and Pascal Favrel}
}
@article {5564,
	title = {Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas},
	journal = {General and Comparative Endocrinology},
	volume = {266},
	year = {2018},
	pages = {110-118},
	type = {regular},
	abstract = {<p class="rtejustify">Although tachykinin-like neuropeptides have been identified in molluscs more than two decades ago, knowledge on their function and signalling has so far remained largely elusive. We developed a cell-based assay to address the functionality of the tachykinin G-protein coupled receptor (Cragi-TKR) in the oyster Crassostrea gigas. The oyster tachykinin neuropeptides that are derived from the tachykinin precursor gene Cragi-TK activate the Cragi-TKR in nanomolar concentrations. Receptor activation is sensitive to Ala-substitution of critical Cragi-TK amino acid residues. The Cragi-TKR gene is expressed in a variety of tissues, albeit at higher levels in the visceral ganglia (VG) of the nervous system. Fluctuations of Cragi-TKR expression is in line with a role for TK signalling in C. gigas reproduction. The expression level of the Cragi-TK gene in the VG depends on the nutritional status of the oyster, suggesting a role for TK signalling in the complex regulation of feeding in C. gigas.</p>
},
	doi = {https://doi.org/10.1016/j.ygcen.2018.05.003},
	author = {Marie-Pierre Dubos and Sven Zels and Julie Schwartz and Jeremy Pasquier and Liliane Schoofs and Pascal Favrel}
}
