%0 Journal Article %J Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology %D 2012 %T In vitro effects of triclosan and methyl-triclosan on the marine gastropod Haliotis tuberculata %A Beatrice Gaume %A Nathalie Bourgougnon %A Stéphanie Auzoux-Bordenave %A Benoit Roig %A Barbara Le Bot %A Gilles Bedoux %K Haliotis tuberculata %X

Triclosan (2,4,4′-trichloro-2′-hydroxy-diphenyl ether; TCS) is an antibacterial agent incorporated in a wide variety of household and personal care products. Because of its partial elimination in sewage treatment plants, \{TCS\} is commonly detected in natural waters and sediments. Moreover, due to its high hydrophobicity, \{TCS\} accumulates in fatty tissues in various aquatic organisms. \{TCS\} can be converted into methyl-triclosan (2,4,4′-trichloro-2′-methoxydiphenyl ether; MTCS) after biological methylation. In this study, the acute cytotoxicity of \{TCS\} and \{MTCS\} in short-term in vitro experiments was assessed on cell cultures from the European abalone Haliotis tuberculata. The results showed that morphology and density of hemocyte are affected from a concentration of 8 μM TCS. Using the \{XTT\} reduction assay, \{TCS\} has been demonstrated to decrease hemocyte metabolism activity in a dose- and time-dependent exposure. The İC50\} was evaluated at 6 μM for both hemocyte and gill cells after a 24 h-incubation with TCS. A significant cytotoxicity of \{MTCS\} was also observed from 4 μM in 24 h-old hemocyte culture. Our results reveal a toxic effect of \{TCS\} and \{MTCS\} on immune (hemocytes) and/or respiratory cells (gill cells) of the abalone, species living in coastal waters areas and exposed to anthropogenic pollution.

%B Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology %V 156 %P 87 - 94 %G eng %U http://www.sciencedirect.com/science/article/pii/S1532045612000610 %R http://dx.doi.org/10.1016/j.cbpc.2012.04.006