%0 Journal Article %J Ecotoxicology %D 2023 %T Impact assessment of metals realeased by aluminium-based galvanic anode on the physiology of the abalone Haliotis tuberculata in controlled conditions %A Alexandre Levallois %A Nivelais, Laureen %A Christelle Caplat %A Jean-Marc Lebel %A Basuyaux, Olivier %A Katherine Costil %A Antoine Serpentini %X To protect metal structures immersed in the sea from corrosion, the galvanic anode cathodic protection system (GACP) is often applied. However, this association leads to continuous oxidation of the galvanic anode and therefore to a release of a metal cocktail in the forms of ions or oxy-hydroxides. Therefore, the main objective of our study was to investigate the toxicity of elements released from the dissolution of an aluminium-based galvanic anode (∼95% Al, ∼5% Zn, <0.1% for In, Cu, Cd, Mn, Fe) on a grazing gastropod, the abalone Haliotis tuberculata. The present study was carried out in complement to other research currently in submission. Gastropods were exposed for 16 weeks (12 weeks of exposure and 4 weeks of decontamination phase) to 6 conditions including a control, 4 concentrations based on total aluminium level (86, 425, 1096 and 3549 µg L−1 ) and a trophic control, corresponding to abalones placed in non-contaminated natural seawater but fed with contaminated algae. The effects of metals on growth, glycogen levels, brix index of hemolymph, MDA levels in digestive gland and gills, hemocyte phagocytic activity, ROS production, lysosomal system and the progress of gametogenesis were investigated throughout the entire exposure allowing the realization of kinetics. The results revealed that the aluminiumbased anode does not seem to have an effect on the health status of the individuals for environmentally realistic concentrations. However, in extreme conditions strong effects were reported on the growth, immune system and reproduction of abalone. %B Ecotoxicology %V 32 %P 438 - 450 %8 Jan-05-2023 %G eng %U https://link.springer.com/10.1007/s10646-023-02652-9 %N 4 %! Ecotoxicology %R 10.1007/s10646-023-02652-9 %0 Journal Article %J Aquatic Toxicology %D 2022 %T Effects of chronic exposure of metals released from the dissolution of an aluminium galvanic anode on the Pacific oyster Crassostrea gigas %A Alexandre Levallois %A Christelle Caplat %A Olivier Basuyaux %A Jean-Marc Lebel %A Antoine Laisney %A Katherine Costil %A Antoine Serpentini %K aluminium %K Bioaccumulation %K Biological effects %K Crassostrea gigas %K galvanic anode %K Oyster %X Among the anthropogenic sources releasing metallic species into the marine environment, the galvanic anode cathodic protection system (GACP) is widely used to protect submerged metallic structures from corrosion. Galvanic anodes are an alloy of metals of which the main component is aluminum or zinc. Very few studies were performed to study their potential biological effects. We investigated the chronic toxicity of an aluminum-based galvanic anode on the Pacific oyster, Crassostrea gigas. Oysters were exposed for 84 days to three concentrations of aluminum (50, 100 and 300 µg L−1) obtained with an electrochemical experimental device simulating the dissolution of a galvanic anode. At different exposure times, we studied a battery of biomarkers of the immune system, reproductive parameters and the metabolic state of the oysters. Results demonstrated a sensitivity of oysters at the highest concentration and some biological effects were observed especially for the malondialdehyde content in the digestive gland after 84 days of exposure. In addition to these biomarkers, the bioaccumulation of the different metals composing the anode was measured in oysters’ tissues. Bivalves bioaccumulated more zinc than aluminum, even if aluminium was present in greater concentrations during exposures. Moreover, exposure time did not influence the bioaccumulation of aluminum in contrast to zinc. %B Aquatic Toxicology %V Volume 249 %G eng %R https://doi.org/10.1016/j.aquatox.2022.106223 %0 Journal Article %J Chemosphere %D 2020 %T Transfer of elements released by aluminum galvanic anodes in a marine sedimentary compartment after long-term monitoring in harbor and laboratory environments %A Christelle Caplat %A Olivier Basuyaux %A Pineau, S %A Deborde, Jonathan %A Grolleau, AM %A S. Leglatin %A Mahaut, Marie-Laure %K Aluminum %K galvanic anode %K Harbor %K Marine sediments %K Quality index %K Zinc %X Cathodic protection by galvanic anodes (GACP) is often used to protect immerged metallic structures in harbor environments, especially GACP employing aluminum-based anodes. To follow a previous study that was performed in a laboratory on Al-anode, two monitoring periods were performed in parallel, one in an in situ environment (in the Port of Calais) for 42 months and the other in a laboratory for 18 months, to evaluate the transfer of metals constituting the Al-anode towards the sedimentary compartment. During each monitoring, two conditions of agitation of water (weak and strong) were compared, and different factors of sediment quality were used to assess the enrichment and potential toxic effects of these released metals. The results showed that the dissolution of Al-anode-induced a greater Zn enrichment of sediment than an Al enrichment. This is in contrast with the abundance of these elements present in the composition of the anode and suggested a potential toxic effect for marine organisms with regards to the discovered Zn level, especially in confined areas. %B Chemosphere %V 239 %P 124720 %G eng %U http://www.sciencedirect.com/science/article/pii/S0045653519319502 %R 10.1016/j.chemosphere.2019.124720 %0 Journal Article %J Ecotoxicology and Environmental Safety %D 2018 %T Toxicological effects of CdSe nanocrystals on the marine diatom Phaeodactylum tricornutum: The first mass spectrometry-based proteomic approach %A Poirier, Isabelle %A Pallud, Marie %A Kuhn, Lauriane %A Hammann, Philippe %A Demortière, Arnaud %A Jamali, Arash %A Chicher, Johana %A Christelle Caplat %A Gallon, Régis Kevin %A Bertrand, Martine %B Ecotoxicology and Environmental Safety %V 152 %P 78 - 90 %8 Jan-05-2018 %G eng %U https://www.sciencedirect.com/science/article/abs/pii/S0147651318300514?via%3Dihub %! Ecotoxicology and Environmental Safety %R 10.1016/j.ecoenv.2018.01.043 %0 Journal Article %J Journal of Proteomics %D 2016 %T Ability of the marine bacterium Pseudomonas fluorescens BA3SM1 to counteract the toxicity of CdSe nanoparticles %A Poirier, Isabelle %A Kuhn, Lauriane %A Demortière, Arnaud %A Mirvaux, Boris %A Hammann, Philippe %A Chicher, Johana %A Christelle Caplat %A Pallud, Marie %A Bertrand, Martine %B Journal of Proteomics %V 148 %P 213 - 227 %8 Jan-10-2016 %G eng %U https://www.sciencedirect.com/science/article/pii/S187439191630330X?via%3Dihub %! Journal of Proteomics %R 10.1016/j.jprot.2016.07.021 %0 Journal Article %J Mar Pollut Bull %D 2016 %T Metal bioaccumulation and physiological condition of the Pacific oyster (Crassostrea gigas) reared in two shellfish basins and a marina in Normandy (northwest France). %A Séguin, A %A Christelle Caplat %A Antoine Serpentini %A Lebel, J M %A Menet-Nedelec, F %A Katherine Costil %X
A 5-month experiment combining a geochemical survey of metals with a bioaccumulation study in batches of Crassostrea gigas was conducted in two shellfish farming areas and a marina in Normandy (France). Various endpoints at different levels of biological organization were studied. ROCCH data showed differences in biota contamination between the two shellfish areas but the present study revealed only slight differences in metallic contamination and biomarkers. By contrast, significantly different values were recorded in the marina in comparison with the two other sites. Indeed, higher levels of Cd, Cu and Zn were measured in the oysters from the marina, and these oysters also showed a poorer physiological condition (e.g., condition index, histopathological alterations and neutral lipid content). For coastal monitoring, the multi-biomarker approach coupled with an assessment of metallic contamination in biota appeared to be suitable for discriminating spatial differences in environmental quality after only a few months of exposure.
%B Mar Pollut Bull %V 106 %P 202-14 %8 2016 May 15 %G eng %N 1-2 %R 10.1016/j.marpolbul.2016.02.068 %0 Journal Article %J water, air & soil pollution %D 2015 %T Impact of Galvanic Anode Dissolution onMetal Trace Element Concentrations in Marine Waters %A Deborde, J %A Refait, P %A Paco Bustamante %A Christelle Caplat %A Basuyaux, O %A Grolleau, AM %A M-L Mahaut %K aluminium %K galvanic anode %K metal transfer %K Seawater %K Zinc %XSubmerged harbor steel structures often employ cathodic protection using galvanic anodes to guard against corrosion. A laboratory experiment, with three different cathodic protection configurations by galvanic aluminum-based anodes, was performed to evaluate the potential metal transfer from the anodic alloy dissolution into the surrounding marine water. The anode dissolution rate is proportional to the imposed current demands and induced a significant Al, In, and Zn transfer in the dissolved and particulate fractions of the corrosion product layers covering the anode surface. These layers were poorly adherent, even under low hydrodynamic conditions. Consequently, at the anode vicinity, the suspended particle matter and dissolved fraction of surrounding marine waters showed strong enrichments in Al and Zn, respectively, the values of which could potentially affect the adjacent biota. After the anode activation period, however, the metal inputs from galvanic anode dissolution are rapidly diluted by seawater renewal. At regional scale, these metal fluxes should be negligible compared to river and wastewater fluxes. These results also showed that it is difficult to assess the impact of the anode dissolution on the concentrations of metals in the natural environment, especially for metals included in trace amounts in the anode alloy (i.e., Cu, Fe, In, Mn, and Si) in the aquatic compartment.
%B water, air & soil pollution %V 226 %P 226-423 %8 11/2015 %G eng %0 Journal Article %J Aquat Toxicol %D 2015 %T Trace metal concentrations in post-hatching cuttlefish Sepia officinalis and consequences of dissolved zinc exposure. %A Le Pabic, C %A Christelle Caplat %A Lehodey Jean-Paul %A Milinkovitch, Thomas %A Siméoni Koueta-Noussithé %A Cosson, Richard Philippe %A Paco Bustamante %XIn this study, we investigated the changes of 13 trace metal and metalloid concentrations (i.e. Ag, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Se, V, Zn) and their subcellular fractionation in juvenile cuttlefish Sepia officinalis reared in controlled conditions between hatching and 2 months post-hatching. In parallel, metallothionein concentrations were determined. Our results highlighted contrasting changes of studied metals. Indeed, As and Fe concentrations measured in hatchlings suggested a maternal transfer of these elements in cuttlefish. The non-essential elements Ag and Cd presented the highest accumulation during our study, correlated with the digestive gland maturation. During the 6 first weeks of study, soluble fractions of most of essential trace metals (i.e. Co, Cr, Cu, Fe, Se, Zn) slowly increased consistently with the progressive needs of cuttlefish metabolism during this period. In order to determine for the first time in a cephalopod how metal concentrations and their subcellular distributions are impacted when the animals are trace metal-exposed, we studied previously described parameters in juveniles exposed to dissolved Zn at environmental (i.e. 50 μg l(-1)) and sublethal (i.e. 200 μg l(-1)) levels. Moreover, oxidative stress (i.e. glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase activities, and lipid peroxidation (LPO)) was assessed in digestive gland and gills after 1 and 2 months exposures. Our results highlighted no or low ability of this stage of life to regulate dissolved Zn accumulation during the studied period, consistently with high sensitivity of this organism. Notably, Zn exposures caused a concentration-dependent Mn depletion in juvenile cuttlefish, and an increase of soluble fraction of Ag, Cd, Cu without accumulation modifications, suggesting substitution of these elements (i.e. Mn, Ag, Cd, Cu) by Zn. In parallel, metallothionein concentrations decreased in individuals most exposed to Zn. Finally, no perturbations in oxidative stress management were detected in gills, whereas modifications of GST, SOD and catalase activity levels were recorded in digestive gland, resulting in an increase of LPO content after a 6-week exposure to low Zn concentration. Altogether, these perturbations are consistent with previously described high sensitivity of juvenile cuttlefish towards Zn. Our results underlined the need to study deeply contamination impact on this animal at this stage of life.
%B Aquat Toxicol %V 159 %P 23-35 %8 2015 Feb %G eng %R 10.1016/j.aquatox.2014.11.012 %0 Journal Article %J Aquatic Toxicology %D 2014 %T The effect of cold stress on the proteome of the marine bacteriumPseudomonas fluorescens BA3SM1 and its ability to copewith metal excess %A Isabelle Poirier %A Lauriane Kuhn %A Christelle Caplat %A Philippe Hammann %A Martine Bertrand %K Cold stress %K Marine strain %K Metal biosorptiona %K Proteomics %K Pseudomonas fluorescens %XThis study examined the effect of cold stress on the proteome and metal tolerance of Pseudomonas fluo-rescens BA3SM1, a marine strain isolated from tidal flat sediments. When cold stress (+10◦C for 36 h)was applied before moderate metal stress (0.4 mM Cd, 0.6 mM Cd, 1.5 mM Zn, and 1.5 mM Cu), growthdisturbances induced by metal, in comparison with respective controls, were reduced for Cd and Znwhile they were pronounced for Cu. This marine strain was able to respond to cold stress through a num-ber of changes in protein regulation. Analysis of the predicted differentially expressed protein functionsdemonstrated that some mechanisms developed under cold stress were similar to those developed inresponse to Cd, Zn, and Cu. Therefore, pre-cold stress could help this strain to better counteract toxicityof moderate concentrations of some metals. P. fluorescens BA3SM1 was able to remove up to 404.3 mgCd/g dry weight, 172.5 mg Zn/g dry weight, and 11.3 mg Cu/g dry weight and its metal biosorption abilityseemed to be related to the bacterial growth phase. Thus, P. fluorescens BA3SM1 appears as a promisingagent for bioremediation processes, even at low temperatures.
%B Aquatic Toxicology %V 157 %P 120-133 %8 10/2014 %G eng