%0 Journal Article %J Chemosphere %D 2015 %T Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation. %A Baillon, Lucie %A Oses, Jennifer %A Pierron, Fabien %A Bureau du Colombier, Sarah %A Caron, Antoine %A Normandeau, Eric %A Lambert, Patrick %A Couture, Patrice %A Labadie, Pierre %A Budzinski, Hélène %A Sylvie Dufour %A Bernatchez, Louis %A Baudrimont, Magalie %X

Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this species. Among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic contaminants on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11weeks of maturation to get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis. Genes associated to contaminants were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish.

%B Chemosphere %V 139 %P 303-309 %8 2015 Jul 6 %G eng %R 10.1016/j.chemosphere.2015.06.007 %0 Journal Article %J Environ Sci Technol %D 2014 %T Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish. %A Pierron, Fabien %A Bureau du Colombier, Sarah %A Moffett, Audrey %A Caron, Antoine %A Peluhet, Laurent %A Daffe, Guillemine %A Lambert, Patrick %A Elie, Pierre %A Labadie, Pierre %A Budzinski, Hélène %A Sylvie Dufour %A Couture, Patrice %A Baudrimont, Magalie %X

There is increasing evidence that pollutants may cause diseases via epigenetic modifications. Epigenetic mechanisms such as DNA methylation participate in the regulation of gene transcription. Surprisingly, epigenetics research is still limited in ecotoxicology. In this study, we investigated whether chronic exposure to contaminants experienced by wild female fish (Anguilla anguilla) throughout their juvenile phase can affect the DNA methylation status of their oocytes during gonad maturation. Thus, fish were sampled in two locations presenting a low or a high contamination level. Then, fish were transferred to the laboratory and artificially matured. Before hormonal treatment, the DNA methylation levels of the genes encoding for the aromatase and the receptor of the follicle stimulating hormone were higher in contaminated fish than in fish from the clean site. For the hormone receptor, this hypermethylation was positively correlated with the contamination level of fish and was associated with a decrease in its transcription level. In addition, whereas gonad growth was associated with an increase in DNA methylation in fish from the clean site, no changes were observed in contaminated fish in response to hormonal treatment. Finally, a higher gonad growth was observed in fish from the reference site in comparison to contaminated fish.

%B Environ Sci Technol %V 48 %P 11688-95 %8 2014 Oct 7 %G eng %N 19 %R 10.1021/es503712c