Key words: Mollusc - Gonad - Gametogenesis - Germinal niche - Gonial mitosis
Structure and functioning of the germinal niche in the oyster Crassostrea gigas: My research concerns the characterization of cellular actors implied in the germinal niche. Mechanisms of stemness and differentiation in this microenvironment are particularly explored. Study of morphology of germ and somatic cells and measure of expressions of molecular markers (KLF, Sox2, Gata1,Vasa, Piwi, Nanos) allows to understand the global architecture of the germinal niche in this alternative hermaphrodite.
Methods used: optical, confocal and electronic microcopies, cellular enrichments, flow cytometry, impedance analysis.
Scientific expertise : Study of germ stem and proliferative compartments in C.gigas. Gametogenesis description in different molluscs (Crassostrea gigas, Corbicula sp, Ruditapes decussatus) by qualitative and quantitative approaches (qualitative histology, quantitative histo-imagery). Cellular enrichments (density gradients, FACS).