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Stéphanie AUZOUX-BORDENAVE

Stéphanie AUZOUX-BORDENAVE's picture

Position

Assistant professor

Affiliation

Sorbonne Université (SU)

Email

bordenav@mnhn.fr

Phone

+33 2 98 50 42 88

Fax

+33 2 98 97 81 24

Localisation

MNHN Station de Concarneau

Team

Team 4: Larval dispersal and communities organization in austral and tropical island systems

Research axis/workshop

Transversal axe Global Change
Workshop Cell Culture

Research topics

My research works focus on the biomineralization processes and ecophysiology of marine mollusc over the development. The Mollusca represent key members of many benthic ecosystems displaying a pelago-benthic life cycle with free planktonic larvae being responsible for dispersion and population distribution. They are among the most diversified metazoan, of ecological and commercial relevance, and their evolutionary success can be in part attributed to the adaptive capacity of the carbonated shell.

Our research objectives are:

  • to better understand the processes of shell morphogenesis by analysing the interaction between mineral and organic components over the life cycle,
  • to characterize how changing ocean conditions (pH, temperature) are likely to affect key biological and developmental responses,

The work is based on the European abalone Haliotis tuberculata, a marine gastropod of commercial interest for aquaculture. Our collaboration since 2005 with the French abalone farm France-Haliotis (Dr. Sylvain Huchette) allows to access the various developmental stages (larval, juveniles, adults) of the abalone life-cycle. The combination of structural and biological approaches allowed to characterize abalone shell microstructure and composition over abalone development and to identify some matrix proteins and enzymes involved in the biological control of shell morphogenesis (Auzoux-Bordenave et al. 2010, Gaume et al., 2011, 2014, Jardillier et al. 2008). I have also developed primary cell cultures to design new biotechnological assays for further studies on abalone pathology, ecotoxicology and shell mineralization (Gaume et al. 2012; O Neill et al. 2013, Pichon et al. 2013). The expression of biomineralization genes is being studied in vivo over the development and in vitro in primary mantle cell cultures of the European abalone.

In the context of climate change, I develop since 2012 pilot experiments to measure the impacts of ocean acidification and warming on the development and shell calcification of marine molluscs. Using the European abalone as model organism, the goals of the project are to investigate the interactive effects of pH and temperature changes on larval morphology and shell formation by combining structural, physiological and molecular approaches. Controlled experiments are underway to check the development of larval shells under decreased pH values, in order to obtain the expression pattern of molecular shell markers and analyse the physical properties of the growing shell.

An ecophysiological approach is being developed in collaboration with the Marine Biology Laboratory (Université Libre de Bruxelles Pr. P. Dubois) in order to evaluate the potential effects of pH and temperature changes on acid-base balance and metabolic parameters.

Teaching

As an associate professor at the University Paris VI (UPMC) I give annually 192h of teaching in animal biology, physiology and cell biology.

I supervise a course “Biomineralisations” within the Master of Science and Technology (UPMC and MNHN).

Administrative responsibilities

  • Membre du groupe d’experts de la 68ème section, UPMC depuis 2011
  • Membre titulaire de la commission de spécialistes 68èmesection du Muséum National d’Histoire Naturelle, Paris (2002-2008).
  • Membre suppléante de la commission de spécialistes 67-68ème sections de l’UPMC, Paris (2001-2004).
  • Correspondante Relations internationales pour la Station Marine de Concarneau (depuis 2011)
  • Responsable des plateaux techniques de culture cellulaire et biochimie/ immunohistochimie de la station marine de Concarneau (depuis 2007).
  • Membre élue du Conseil du Département Milieux et Peuplements Aquatiques, MNHN, depuis 2008

Expertise and Valorization

  • Membre du comité d’organisation de la conférence de la "European Marine Biotechnology Society", Concarneau, 1-3 septembre 2009. Chairman de la session ‘Marine Invertebrate Cell Cultures
  • Membre du Pôle de Compétitivité Mer-Bretagne, depuis 2006
  • Activités de transfert des connaissances dans les domaines de l’aquaculture durable et la préservation des ressources marines en collaboration avec les acteurs institutionnels et professionnels de la région Bretagne
  • Expertise en culture cellulaire d’invertébrés marins et développement de recherches finalisées :  biotests in vitro et applications en pathologie, écotoxicologie….
  • Coordination de l’axe méthodologique transversal de l’UMR BOREA « Cultures cellulaires chez des modèles aquatiques non conventionnels »