@article {Duplat2006,
	title = {{Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera}},
	journal = {Febs Letters},
	volume = {580},
	year = {2006},
	pages = {2435{\textendash}2441},
	abstract = {Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95-99\% calcium carbonate and 1-5\%, organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.},
	issn = {0014-5793},
	doi = {10.1016/j.febslet.2006.03.077},
	author = {Duplat, D and Puissegur, M and Bedouet, L and Rousseau, A and Boulzaguet, H and Milet, C and Sellos, D and Van Wormhoudt, A and Lopez, E}
}
