@article {Gricourt_etal2003,
	title = {{Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor}},
	journal = {General and Comparative Endocrinology},
	volume = {134},
	number = {1},
	year = {2003},
	pages = {44{\textendash}56},
	abstract = {The involvement of molecules belonging to the insulin/IGF family in regulation of growth has been investigated in the Pacific oyster <i>Crassostrea gigas</i>. In vitro biological effects of human recombinant IGF-1 (hrIGF-1) on mantle edge cells, involved in oyster shell and soft body growth, were studied over an annual cycle. In mantle edge cells hrIGF-1 stimulates protein synthesis of 56 {\textpm} 5.1\% over basal for 10-10 M in September with in addition a clear dose-effect corresponding to the highest shell growth period, and 57.5 {\textpm} 3.45\% over basal for 10-11 M in March and 51 {\textpm} 5.4\% over basal for 10-10 M in April corresponding to the period of mantle growth. These insulin-like effects were associated with the expression of a recently identified <i>C. gigas</i> insulin receptor-related receptor (CIR) in mantle edge cells as demonstrated by RT-PCR. Moreover, in situ hybridisation (ISH) confirmed this expression at the level of the inner and outer epithelia involved in mantle growth and shell formation.},
	issn = {0016-6480},
	author = {Gricourt, L and Bonnec, G and Boujard, D and Mathieu, M and Kristell Kellner-Cousin}
}
