@article {3508,
	title = {Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).},
	journal = {Fish Shellfish Immunol},
	volume = {40},
	year = {2014},
	month = {2014 Oct},
	pages = {362-73},
	abstract = {<p>Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.</p>
},
	issn = {1095-9947},
	doi = {10.1016/j.fsi.2014.07.020},
	author = {Le Pabic, C and Goux, Didier and Guillamin, Maryline and Safi, Georges and Jean-Marc Lebel and Sim{\'e}oni Koueta-Noussith{\'e} and Antoine Serpentini}
}
