@article {3828,
	title = {Transcriptome analysis reveals strong and complex antiviral response in a mollusc.},
	journal = {Fish Shellfish Immunol},
	year = {2015},
	month = {2015 May 22},
	abstract = {<p>Viruses are highly abundant in the oceans, and how filter-feeding molluscs without adaptive immunity defend themselves against viruses is not well understood. We studied the response of a mollusc Crassostrea gigas to Ostreid herpesvirus 1 {\textmu}Var (OsHV-1μVar) infections using transcriptome sequencing. OsHV-1μVar can replicate extremely rapidly after challenge of C.\&nbsp;gigas as evidenced by explosive viral transcription and DNA synthesis, which peaked at 24 and 48\&nbsp;h post-inoculation, respectively, accompanied by heavy oyster mortalities. At 120\&nbsp;h post-injection, however, viral gene transcription and DNA load, and oyster mortality, were greatly reduced indicating an end of active infections and effective control of viral replication in surviving oysters. Transcriptome analysis of the host revealed strong and complex responses involving the activation of all major innate immune pathways that are equipped with expanded and often novel receptors and adaptors. Novel Toll-like receptor (TLR) and MyD88-like genes lacking essential domains were highly up-regulated in the oyster, possibly interfering with TLR signal transduction. RIG-1/MDA5 receptors for viral RNA, interferon-regulatory factors, tissue necrosis factors and interleukin-17 were highly activated and likely central to the oyster{\textquoteright}s antiviral response. Genes related to anti-apoptosis, oxidation, RNA and protein destruction were also highly up-regulated, while genes related to anti-oxidation were down-regulated. The oxidative burst induced by the up-regulation of oxidases and severe down-regulation of anti-oxidant genes may be important for the destruction of viral components, but may also exacerbate oyster mortality. This study provides unprecedented insights into antiviral response in a mollusc. The mobilization and complex regulation of expanded innate immune-gene families highlights the oyster genome{\textquoteright}s adaptation to a virus-rich marine environment.</p>
},
	issn = {1095-9947},
	doi = {10.1016/j.fsi.2015.05.023},
	author = {He, Yan and Jouaux, Aude and Ford, Susan E and Christophe Lelong and Pascal Sourdaine and Mathieu, Michel and Guo, Ximing}
}
