@article {4115,
	title = {Cadmium exposure affects the expression of genes involved in skeletogenesis and stress response in gilthead sea bream larvae.},
	journal = {Fish Physiol Biochem},
	volume = {39},
	year = {2013},
	month = {2013 Jun},
	pages = {649-59},
	abstract = {<p>Gilthead sea bream larvae (Sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of Cd(2+) (0.1, 5 and 10 mg/L) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (metallothionein-mt, glutathione peroxidase 1-gpx1), stress response (heat shock protein 70-hsp70, tumour necrosis factor α-tnfα) and ossification (osteocalcin-oc) processes. For this purpose, specimens of 47 dph were exposed first for 72 h from 0.1 to 20 mg/L of Cd(2+) in order to evaluate the median lethal concentration (LC(50)) for this metal, which was determined at 15.32 mg/L. Considering the results regarding the relative transcript levels of gpx1 and hsp70, Cd(2+) at any of the tested levels (0.1, 5 and 10 mg/L) did not induce oxidative stress in gilthead sea bream larvae, whereas relative transcript levels of mt were increased at 5 and 10 mg/L of Cd(2+) probably to detoxify this metal excess. Relative transcript levels of tnfα were not level dependent and were down-regulated in larvae exposed to 5 and 10 mg/L of Cd(2+). At those concentrations, transcript levels of oc were down-regulated suggesting a disruption in bone mineralization. Results from this study provided insights in some molecular mechanisms underlying Cd(2+)-induced toxicity in fish at early stages of development. This is the first study to show that cadmium contamination can depress oc expression in teleosts.</p>
},
	keywords = {Animals, Cadmium, DNA Primers, Dose-Response Relationship, Drug, Gene Expression Regulation, Larva, Lethal Dose 50, Osteogenesis, Real-Time Polymerase Chain Reaction, Sea Bream, Stress, Physiological},
	issn = {1573-5168},
	doi = {10.1007/s10695-012-9727-9},
	author = {Sassi, Asma and Darias, Maria and Said, Khaled and Messaoudi, Imed and Gisbert, E}
}
