@article {4117,
	title = {Molecular regulation of both dietary vitamin A and fatty acid absorption and metabolism associated with larval morphogenesis of Senegalese sole (Solea senegalensis).},
	journal = {Comp Biochem Physiol A Mol Integr Physiol},
	volume = {161},
	year = {2012},
	month = {2012 Feb},
	pages = {130-9},
	abstract = {<p>The present study aimed to deepen the understanding of molecular mechanisms governing the absorption and metabolism of some nutrients, growth and development in larvae of Senegalese sole (Solea senegalensis) fed with Artemia enriched with Easy Selco (ES, INVE) or Aquagrow Gold (AGG, ABN), which mainly differed in their vitamin A (VA) content and fatty acid composition. The expression profile of genes involved in VA metabolism (crbp2, rbp, crabp1), lipid transport (i-fabp, l-fabp), nuclear receptors for VA and fatty acids (rarα1, rxrα, pparβ), growth (igf1, igf2 and their receptor igf1r) and development (bgp) was analyzed at 22, 30 and 38 days post hatching. The main results suggested that the amount of VA absorbed by larvae is controlled at the intestinal level by crbp2 in both groups, preventing excessive accumulation of this vitamin in the target tissues. The stable expression of i-fabp in the ES group with age could cause an excessive fat accumulation in the intestine inducing, in turn, the steatosis found in the liver and vascular system of these specimens. In liver, the regulation of rbp and fabp expression reflected the status of the physiological functions demanding VA and lipids. The findings revealed that dietary composition induced different strategies for VA and lipid absorption and metabolism affecting, in turn, larval development, growth and health.</p>
},
	keywords = {Absorption, Animals, Diet, fatty acids, Feeding Behavior, Fish Proteins, Flatfishes, Gene Expression Regulation, Larva, Lipid Metabolism, Morphogenesis, Senegal, Vitamin A},
	issn = {1531-4332},
	doi = {10.1016/j.cbpa.2011.10.001},
	author = {Darias, Maria and Boglino, A and Manchado, Manuel and Ortiz-Delgado, Juan Bosco and Est{\'e}vez, A and Andree, K B and Gisbert, E}
}
