@article {4130,
	title = {The effect of dietary arachidonic acid during the Artemia feeding period on larval growth and skeletogenesis in Senegalese sole, Solea senegalensis},
	journal = {Journal of Applied Ichthyology},
	volume = {28},
	year = {2012},
	pages = {411{\textendash}418},
	abstract = {<p>To improve the understanding of the incidence of skeletal deformities in Senegalese sole (Solea senegalensis), a feeding trial was carried out to evaluate the effect of increasing dietary arachidonic acid (ARA) levels during the Artemia feeding period on larval growth and skeletogenesis. Larvae were fed from 8 to 50\&nbsp;days post hatching (dph) with Artemia nauplii enriched with three different levels of ARA: ARA-Low, ARA-Medium and ARA-High (1.0, 4.5 and 7.0\% ARA of total fatty acids, respectively). Increasing levels of dietary ARA did not affect significantly the survival. However, it influenced growth; larvae from the ARA-M group presented the highest values of final standard length and dry weight (11.36\&nbsp;{\textpm}\&nbsp;0.47\&nbsp;mm and 5.86\&nbsp;{\textpm}\&nbsp;1.06\&nbsp;mg at 50\&nbsp;dph, respectively) and larvae from the ARA-H group the lowest ones (9.53\&nbsp;{\textpm}\&nbsp;0.27\&nbsp;mm and 2.47\&nbsp;{\textpm}\&nbsp;0.26\&nbsp;mg at 50\&nbsp;dph). The skeleton of larvae fed the ARA-M diet tended to be more calcified at 15\&nbsp;dph than that of larvae fed ARA-L and ARA-H diets. Larvae from the ARA-M group tended to show a higher incidence of fusion of hypurals 3 and 4 (34.4\&nbsp;{\textpm}\&nbsp;3.1\%) at 50\&nbsp;dph than the larvae from the other groups (18.7\&nbsp;{\textpm}\&nbsp;1.6\%, in average). The latter results reflected a trend to a higher degree of skeletal development rather than a deformity, this being in agreement with the higher larval growth of this group. Besides, ARA levels did not affect significantly the incidence of total skeletal deformities (41.4\&nbsp;{\textpm}\&nbsp;1.5\% in average). Larvae from all dietary treatments mostly displayed fusions of the vertebra 43 and 44 (32.2\&nbsp;{\textpm}\&nbsp;1.3\% in average). The amount of 4.5\% of total fatty acid of dietary ARA during Artemia feeding period promoted the best growth and proper skeletogenesis in Senegalese sole larvae.</p>
},
	issn = {1439-0426},
	doi = {10.1111/j.1439-0426.2012.01977.x},
	url = {http://dx.doi.org/10.1111/j.1439-0426.2012.01977.x},
	author = {Boglino, A and Darias, Maria and Est{\'e}vez, A and Andree, K B and Gisbert, E}
}
