@article {4141,
	title = {Dietary vitamin \{D3\} affects digestive system ontogenesis and ossification in European sea bass (Dicentrachus labrax, Linnaeus, 1758)},
	journal = {Aquaculture},
	volume = {298},
	year = {2010},
	pages = {300 - 307},
	abstract = {<p>The influence of dietary vitamin \{D3\} (VD3) on survival, growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45 days post hatching. Diets contained 4\% of the standard vitamin mix (VM) recommended by the National Research Council (NRC) and incorporated 0, 19.2, 38.4, or 140 {\.I}U\} of \{VD3\} per gram of diet to give VD-0, VD-1, VD-2 and VD-3 dietary treatments, respectively. The present study revealed for the first time an impact of dietary \{VD3\} on the sea bass digestive system ontogenesis that consequently conditioned the ossification process and morphogenesis. All dietary \{VD3\} levels were in the {\textquotedblleft}adequate range{\textquotedblright} based on larval survival. Nevertheless, growth, intestinal maturation and ossification at the end of the larval stage were harmed by the minimum dose of \{VD3\} tested and resulted in the appearance of malformations. VD-2 and VD-3 groups showed satisfactory growth and ossification levels at the end of the larval period. However, results of enzymatic activity and expression of genes involved in the \{VD3\} pathway (bone morphogenetic protein 4, osteocalcin, vitamin D receptors and transient receptor potential cation channel-subfamily V, member 6-) gave evidence of complications during the ossification process as revealed by the high percentage of deformed larvae. A \{VD3\} level of 19.2 IU/g diet appeared necessary to obtain harmonious larval morphogenesis.</p>
},
	keywords = {Gene Expression},
	issn = {0044-8486},
	doi = {http://dx.doi.org/10.1016/j.aquaculture.2009.11.002},
	url = {http://www.sciencedirect.com/science/article/pii/S0044848609008904},
	author = {Darias, Maria and Mazurais, D and Koumoundouros, Giorgo and Glynatsi, Nomiki and Christodoulopoulou, S and Huelvan, Christine and Desbruyeres, Elisabeth and Le Gall, Marie M and Quazuguel, Patrick and Cahu, Chantal L and Zambonino-Infante, J}
}
