@article {4144,
	title = {Senegalese sole (Solea senegalensis) metamorphic larvae are more sensitive to pseudo-albinism induced by high dietary arachidonic acid levels than post-metamorphic larvae},
	journal = {Aquaculture},
	volume = {433},
	year = {2014},
	pages = {276 - 287},
	abstract = {<p>Abstract High dietary levels of arachidonic acid (ARA) and its relative proportions with eicosapentaenoic acid (EPA), fed during early larval stages, have been associated with malpigmentation in various flatfish species. This study investigated whether the nutritional induction of pigmentary disorders at larval stages was related to a specific larval period of increased sensitivity to \{ARA\} in Senegalese sole (Solea senegalensis Kaup, 1858). Senegalese sole larvae were fed high dietary \{ARA\} levels during pre- and pro-metamorphosis (2{\textendash}15 dph) and/or post-metamorphosis (15{\textendash}50 dph). Larval tissues reflected the dietary fatty acid composition. Malpigmentations were significantly related to elevated dietary and larval \{ARA\} contents and ARA/EPA ratio. This study reports evidence for a {\textquotedblleft}pigmentation window{\textquotedblright}, with a higher larval sensitivity to dietary \{ARA\} during pre- and pro-metamorphosis than post-metamorphosis. High dietary \{ARA\} fed to larvae during pre-metamorphosis enhanced survival, but did not affect growth nor eye migration. The aspect and density of melanophores in the skin of the ocular side of ARA-induced pseudo-albinos were significantly reduced in comparison to normally pigmented individuals, even more in the pseudo-albino fish fed high dietary \{ARA\} levels during the pre-metamorphic stage. Pseudo-albino fish fed high dietary \{ARA\} levels during post-metamorphosis showed higher concentrations of 2- and 3-series prostaglandins (PGE2 and PGE3) than normally pigmented specimens fed the same diets. An increased sensitivity to ARA-induced malpigmentations has been identified at pre-metamorphosis and early metamorphosis in Senegalese sole. Supplying high dietary \{ARA\} amounts imbalanced the dietary ARA/EPA ratio and disrupted the relative concentrations of derived \{PGE2\} and PGE3, resulting in 20 to 81.7\% pseudo-albino individuals, depending on the dietary treatment. The administration of high levels of dietary \{ARA\} at different developmental stages did not only affect the incidence of animals with pigmentary disorders, but it also affect the melanophore density and skin aspect in normally pigmented and pseudoalbino fish as image segmentation and texture analyses indicated.</p>
},
	keywords = {Prostaglandins},
	issn = {0044-8486},
	doi = {http://dx.doi.org/10.1016/j.aquaculture.2014.06.012},
	url = {http://www.sciencedirect.com/science/article/pii/S0044848614003056},
	author = {Boglino, A and Wishkerman, A and Darias, Maria and de la Iglesia, P and Andree, K B and Gisbert, E and Est{\'e}vez, A}
}
