@article {4203,
	title = {Growth, survival and the histology of the digestive tract of juvenile Osteoglossum bicirrhosum (Cuvier, 1829) fed three diets containing different protein and lipid levels},
	journal = {Journal of Applied Ichthyology},
	volume = {31},
	year = {2015},
	pages = {67{\textendash}73},
	abstract = {<p>The influence of three commercial diets with different protein and lipid contents (40\&nbsp;:\&nbsp;14, 45\&nbsp;:\&nbsp;8, 48\&nbsp;:\&nbsp;8 protein\&nbsp;:\&nbsp;lipid {\textendash} P\&nbsp;:\&nbsp;L, in \% dry weight of diet) on growth performance, survival and the histology of the liver and intestine was analysed in early juveniles of silver arowana, Osteoglossum\&nbsp;bicirrhosum, reared in captivity. Individuals (initial wet\&nbsp;weight{\textendash}Wi\&nbsp;=\&nbsp;1.07\&nbsp;{\textpm}\&nbsp;0.04\&nbsp;g; total length{\textendash}TL\&nbsp;= 6.0\&nbsp;{\textpm} 0.17\&nbsp;cm) were raised in triplicate (n\&nbsp;=\&nbsp;12 fish per tank) at 26.2\&nbsp;{\textpm}\&nbsp;0.1{\textdegree}C and fed one of three commercial diets over 60\&nbsp;days. At the end of the trial, survival (90.5\&nbsp;{\textpm}\&nbsp;3.3\%) and condition factor (0.5\&nbsp;{\textpm}\&nbsp;0.02) were not affected by dietary treatments, whereas specific growth rate, TL and W gain were lower in animals fed the 40P\&nbsp;:\&nbsp;14L diet. Histological analysis revealed a larger accumulation of lipid droplets in the intestines of the group supplied with the 40P\&nbsp;:\&nbsp;14L diet, as well as a larger surface of hepatic lipid vacuoles compared to the other treatments, although no signs of steatosis were observed. Juveniles fed the 48P\&nbsp;:\&nbsp;8L diet displayed the lowest liver lipid accumulation; however, a protein content higher than 45\% did not improve growth performance. The intestine and liver lipid accumulation of individuals fed the 45P\&nbsp;:\&nbsp;8L diet might indicate a more balanced lipidic metabolism. Intestine and liver histologies proved to be useful markers to identify the nutritional condition in juveniles of O.\&nbsp;bicirrhosum, even when differences in nutritional composition were subtle (i.e. protein variation of 3\%).</p>
},
	issn = {1439-0426},
	doi = {10.1111/jai.12977},
	url = {http://dx.doi.org/10.1111/jai.12977},
	author = {Darias, Maria and G{\'o}mez, M. A. and Salvador Tello and Gisbert, E}
}
