@article {9276, title = {Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark (Scyliorhinus canicula)}, journal = {Journal of Proteome Research}, volume = {22}, year = {2023}, month = {Jul-07-2023}, pages = {2477 - 2492}, issn = {1535-3893}, doi = {https://doi.org/10.1021/acs.jproteome.3c00206}, url = {https://pubs.acs.org/doi/10.1021/acs.jproteome.3c00206}, author = {Jeanne, Fabian and Bernay, Beno{\^\i}t and Pascal Sourdaine} } @article {6626, title = {K092A and K092B, Two Peptides Isolated from the Dogfish (Scyliorhinus canicula L.), with Potential Antineoplastic Activity Against Human Prostate and Breast Cancer Cells}, journal = {Marine Drugs}, volume = {17}, year = {2019}, month = {Jan-12-2019}, pages = {672}, doi = {10.3390/md17120672}, url = {https://www.mdpi.com/1660-3397/17/12/672}, author = {Bosseboeuf, Adrien and Baron, Amandine and Duval, Elise and Aude Gautier and Pascal Sourdaine and Auvray, Pierr{\"\i}ck} } @article {6635, title = {A Potential Antineoplastic Peptide of Human Prostate Cancer Cells Derived from the Lesser Spotted Dogfish (Scyliorhinus canicula L.)}, journal = {Marine Drugs}, volume = {17}, year = {2019}, month = {Jan-10-2019}, pages = {585}, doi = {10.3390/md17100585}, url = {https://www.mdpi.com/1660-3397/17/10/585}, author = {Bosseboeuf, Adrien and Baron, Amandine and Duval, Elise and Aude Gautier and Pascal Sourdaine and Auvray, Pierr{\"\i}ck} } @article {6634, title = {The nanos1 gene was duplicated in early Vertebrates and the two paralogs show different gonadal expression profiles in a shark}, journal = {Scientific Reports}, volume = {8}, year = {2018}, month = {Jan-12-2018}, doi = {10.1038/s41598-018-24643-1}, url = {http://www.nature.com/articles/s41598-018-24643-1}, author = {Gribouval, Laura and Pascal Sourdaine and Lareyre, Jean-Jacques and Bellaiche, Johanna and Le Gac, Florence and Mazan, Sylvie and Guiardiere, C{\'e}cile and Auvray, Pierr{\"\i}ck and Aude Gautier} } @inbook {6630, title = {Spermatogenesis and Spermiogenesis in Elasmobranchs, a Short Overview}, booktitle = {Encyclopedia of Reproduction, Second Edition}, volume = {6}, year = {2018}, pages = {305 - 312}, publisher = {Elsevier}, organization = {Elsevier}, isbn = {9780128151457}, doi = {10.1016/B978-0-12-809633-8.20572-4}, url = {https://linkinghub.elsevier.com/retrieve/pii/B9780128096338205724}, author = {Pascal Sourdaine and Aude Gautier and Gribouval, Laura} } @article {5031, title = {Dynamics of DNA methylomes underlie oyster development}, journal = {PLoS Genetics}, volume = {13}, year = {2017}, month = {06/2017}, pages = {e1006807}, type = {Research Paper}, url = {https:// doi.org/10.13 71/journal.p gen.1006807}, author = {Guillaume Rivi{\`e}re and Yan He and Samuele Tecchio and Elizabeth Crowell and Micha{\"e}l Gras and Pascal Sourdaine and Guo, Ximing and Pascal Favrel} } @article {5238, title = {Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve, Pecten maximus (Linnaeus, 1758)}, journal = {J. of Molluscan Studies}, volume = {82}, year = {2016}, month = {11/2016}, pages = {575-584}, type = {research}, abstract = {

In order to investigate the mechanisms of final maturation and capacitation of spermatozoa in Pecten maximus, we used a 2D proteomic approach coupled with MALDI-TOF/TOF mass spectrometry (MS) and bioinformatics search against the Pecten database, to set up a reference map of the proteome of spawned spermatozoa, and identified 133 proteins on the basis of the EST database. These proteins are mainly involved in energy production, ion and electron transport (44\%), cell movement (22\%) and developmental processes (10\%). Comparison between proteomes of spermatozoa collected before and after transit through the genital ducts of P. maximus led to the identification of differentially expressed proteins. Most of them are associated with energy metabolism (aconitate hydratase, malate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase), indicating important modifications of energy production during transit in gonoducts, potentially linked with acquisition of sperm motility. Three proteins involved in cell movement (Tektin-2, tubulin and microtubule-associated protein RP/EB family member 3) were down-regulated in spermatozoa stripped from the gonad. 40S ribosomal protein SA, involved in maturation of 40S ribosomal subunits, was also found to be down-regulated in spermatozoa obtained by induced spawning, suggesting reduction of the effi- ciency of RNA translation, a characteristic of late spermatozoon differentiation. These results confirm that maturation processes of P. maximus spermatozoa during transit through the gonoduct involve RNA translation, energy metabolism and structural proteins implicated in cell movement. Spermatozoa maturation processes clearly differ between P. maximus and gonochoric or alternately hermaphroditic bivalves, potentially in relation to reproductive strategies: the final maturation of the spermatozoon along the genital tract probably contributes to reduction of autofertilization in this simultaneously hermaphroditic species

}, doi = {doi:10.1093/mollus/eyw028}, author = {Boonmee, A and Clothilde Berthelin and Kingtong, S and Pauletto, M and Bernay, B and Suquet, M and Pascal Sourdaine and Kellner K} } @article {4530, title = {Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve, Pecten maximus (Linnaeus, 1758)}, journal = {Journal of Molluscan Studies}, year = {2016}, month = {09/2016}, pages = {1-10}, author = {Boonmee, A and Clothilde Heude-Berthelin and Kingtong, S and Pauletto, M and Bernay, B and Adeline, B and Suquet, M and Pascal Sourdaine and Kristell Kellner-Cousin} } @article {3828, title = {Transcriptome analysis reveals strong and complex antiviral response in a mollusc.}, journal = {Fish Shellfish Immunol}, year = {2015}, month = {2015 May 22}, abstract = {

Viruses are highly abundant in the oceans, and how filter-feeding molluscs without adaptive immunity defend themselves against viruses is not well understood. We studied the response of a mollusc Crassostrea gigas to Ostreid herpesvirus 1 {\textmu}Var (OsHV-1μVar) infections using transcriptome sequencing. OsHV-1μVar can replicate extremely rapidly after challenge of C.\ gigas as evidenced by explosive viral transcription and DNA synthesis, which peaked at 24 and 48\ h post-inoculation, respectively, accompanied by heavy oyster mortalities. At 120\ h post-injection, however, viral gene transcription and DNA load, and oyster mortality, were greatly reduced indicating an end of active infections and effective control of viral replication in surviving oysters. Transcriptome analysis of the host revealed strong and complex responses involving the activation of all major innate immune pathways that are equipped with expanded and often novel receptors and adaptors. Novel Toll-like receptor (TLR) and MyD88-like genes lacking essential domains were highly up-regulated in the oyster, possibly interfering with TLR signal transduction. RIG-1/MDA5 receptors for viral RNA, interferon-regulatory factors, tissue necrosis factors and interleukin-17 were highly activated and likely central to the oyster{\textquoteright}s antiviral response. Genes related to anti-apoptosis, oxidation, RNA and protein destruction were also highly up-regulated, while genes related to anti-oxidation were down-regulated. The oxidative burst induced by the up-regulation of oxidases and severe down-regulation of anti-oxidant genes may be important for the destruction of viral components, but may also exacerbate oyster mortality. This study provides unprecedented insights into antiviral response in a mollusc. The mobilization and complex regulation of expanded innate immune-gene families highlights the oyster genome{\textquoteright}s adaptation to a virus-rich marine environment.

}, issn = {1095-9947}, doi = {10.1016/j.fsi.2015.05.023}, author = {He, Yan and Jouaux, Aude and Ford, Susan E and Christophe Lelong and Pascal Sourdaine and Mathieu, Michel and Guo, Ximing} } @article {Bosseboeuf_etal2014, title = {{Characterization of spermatogonial markers in the mature testis of the dogfish (Scyliorhinus canicula L.)}}, journal = {Reproduction}, volume = {147}, number = {147}, year = {2014}, month = {01/2014}, pages = {125{\textendash}139}, author = {Bosseboeuf, A and Aude Gautier and Auvray, P and Mazan, S and Pascal Sourdaine} } @article {3497, title = {Maintenance of potential spermatogonial stem cells in vitro by GDNF treatment in a chondrichthyan model (Scyliorhinus canicula L.).}, journal = {Biol Reprod}, volume = {91}, year = {2014}, month = {2014 Oct}, pages = {91}, abstract = {

Previous work in dogfish, Scyliorhinus canicula, has identified the testicular germinative area as the spermatogonial stem cell niche. In the present study, an in vitro co-culture system of spermatogonia and somatic cells from the germinative area was developed. Long-term maintenance of spermatogonia has been successful, and addition of GDNF has promoted the development of clones of spermatogonia expressing stem cell characteristics such as alkaline phosphatase activity and has allowed maintenance of self-renewal in spermatogonia for at least 5 mo under culture conditions, notably by decreasing cell apoptosis. Furthermore, clones of spermatogonia expressed the receptor of GDNF, GFRalpha1, which is consistent with the effect of GDNF on cells despite the lack of identification of a GDNF sequence in the dogfish{\textquoteright}s transcriptome. However, a sequence homologous to artemin has been identified, and in silico analysis supports the hypothesis that artemin could replace GDNF in the germinative area in dogfish. This study, as the first report on long-term in vitro maintenance of spermatogonia in a chondrichthyan species, suggests that the GFRalpha1 signaling function in self-renewal of spermatogonial stem cells is probably conserved in gnathostomes.

}, issn = {1529-7268}, doi = {10.1095/biolreprod.113.116020}, author = {Aude Gautier and Bosseboeuf, A and Auvray, P and Pascal Sourdaine} }