@article {6940,
	title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes- Application for Cartilage Engineering.},
	journal = {Mar Biotechnol (NY)},
	volume = {20},
	year = {2018},
	month = {2018 Aug},
	pages = {436-450},
	abstract = {<p>The shells of the bivalve mollusks are organo-mineral structures predominantly composed of calcium carbonate, but also of a minor organic matrix, a mixture of proteins, glycoproteins, and polysaccharides. These proteins are involved in mineral deposition and, more generally, in the spatial organization of the shell crystallites in well-defined microstructures. In this work, we extracted different organic shell extracts (acid-soluble matrix, acid-insoluble matrix, water-soluble matrix, guanidine HCl/EDTA-extracted matrix, referred as ASM, AIM, WSM, and EDTAM, respectively) from the shell of the scallop Pecten maximus and studied their biological activities on human articular chondrocytes (HACs). We found that these extracts differentially modulate the biological activities of HACs, depending on the type of extraction and the concentration used. Furthermore, we showed that, unlike ASM and AIM, WSM promotes maintenance of the chondrocyte phenotype in monolayer culture. WSM increased the expression of chondrocyte-specific markers (aggrecan and type II collagen), without enhancing that of the main chondrocyte dedifferentiation marker (type I collagen). We also demonstrated that WSM could favor redifferentiation of chondrocyte in collagen sponge scaffold in hypoxia. Thus, this study suggests that the organic matrix of Pecten maximus, particularly WSM, may contain interesting molecules with chondrogenic effects. Our research emphasizes the potential use of WSM of Pecten maximus for cell therapy of cartilage.</p>
},
	keywords = {Aged, Aged, 80 and over, Aggrecans, Animal Shells, Animals, Cell Differentiation, Cells, Cultured, Chondrocytes, Collagen Type II, Extracellular Matrix, Gene Expression Profiling, Humans, Middle Aged, Pecten, Phenotype},
	issn = {1436-2236},
	doi = {10.1007/s10126-018-9807-7},
	author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Antoine Serpentini and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence}
}
@article {8720,
	title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes{\textemdash} Application for Cartilage Engineering},
	journal = {Marine Biotechnology},
	volume = {20},
	year = {2018},
	month = {Jan-08-2018},
	pages = {436 - 450},
	issn = {1436-2228},
	doi = {10.1007/s10126-018-9807-7},
	url = {http://link.springer.com/10.1007/s10126-018-9807-7},
	author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Serpentini, Antoine and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence}
}
@article {8719,
	title = {Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro},
	journal = {Cytotechnology},
	volume = {69},
	year = {2017},
	month = {Jan-10-2017},
	pages = {815 - 829},
	issn = {0920-9069},
	doi = {10.1007/s10616-017-0096-1},
	url = {http://link.springer.com/10.1007/s10616-017-0096-1},
	author = {Latire, Thomas and Legendre, Florence and Bouyoucef, Mouloud and Marin, Fr{\'e}d{\'e}ric and Carreiras, Franck and Rigot-Jolivet, Muriel and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Serpentini, Antoine}
}
@article {3427,
	title = {Shell extracts from the marine bivalve Pecten maximus regulate the synthesis of extracellular matrix in primary cultured human skin fibroblasts.},
	journal = {PLoS One},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {e99931},
	abstract = {<p>Mollusc shells are composed of more than 95\% calcium carbonate and less than 5\% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the -112/-61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.</p>
},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0099931},
	author = {Latire, T and Legendre, Florence and Bigot, Nicolas and Carduner, Ludovic and Kellouche, Sabrina and Bouyoucef, Mouloud and Carreiras, Franck and Marin, F and Jean-Marc Lebel and Gal{\'e}ra, Philippe and Antoine Serpentini}
}
