@article {6631, title = {In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata}, journal = {Fish and Shellfish Immunology}, year = {2020}, abstract = {Glyphosate-based herbicides are among the most produced and widely-used herbicides.\ Studies have shown that commercial formulations and adjuvants may be more toxic to non-target organisms than the active ingredients alone, but the mechanisms of action of these\ chemicals remain unclear. The aim of this study was to investigate the in vitro effects of\ glyphosate, a commercial formulation and adjuvant alone using primary culture of hemocytes\ from the European abalone Haliotis tuberculata, a commonly farmed shellfish. Glyphosate\ was found to have negligible effects on viability, phagocytic activities and lysosome stability\ even with very high doses (i.e. 100 mg L-1). By contrast, greater effects on viability were\ observed for the commercial formulation and adjuvant alone, with EC50 values of 41.42 mg L-1 and 1.85 mg L-1, respectively. These results demonstrate that the toxic sublethal effects (i.e.\ phagocytic activity and destabilization of lysosomal membranes) of formulated glyphosate
came from adjuvants and suggest they may be related to cell and organelle membrane\ destabilization.}, keywords = {glyphosate, Haliotis tuberculata, Hemocytes, neutral red retention assay, Phagocytosis, POEAs, viability assay}, doi = {10.1016/j.fsi.2020.02.058}, url = {https://doi.org/10.1016/j.fsi.2020.02.058}, author = {Mottier, Antoine and Antoine Serpentini and Dallas, Lorna and James, Ad{\`e}le and Lebel, Jean-Marc and Katherine Costil} } @article {3490, title = {Characterisation and expression of the biomineralising gene Lustrin A during shell formation of the European abalone Haliotis tuberculata}, journal = {Comparative Biochemistry and Physiology , Part B}, volume = {169}, year = {2014}, month = {2014}, pages = {1-8}, type = {Research article}, abstract = {

The molluscan shell is a remarkable product of a highly biomineralisation process, and is composed of calcium carbonate most commonly in the form of calcite or aragonite. The exceptional mechanical properties of this biomaterial are imparted by the embedded organic matrix which is secreted by the underlying mantle tissue. While many shell-matrix proteins have already been identified within adult molluscan shell, their presence and role in the early developmental stages of larval shell formation are not well understood. In the European abalone Haliotis tuberculata, the shell first forms in the early trochophore larva and develops into a mineralised protoconch in the veliger. Following metamorphosis, the juvenile shell rapidly changes as it becomes flattened and develops a more complex crystallographic profile including an external granular layer and an internal nacreous layer. Among the matrix proteins involved in abalone shell formation, Lustrin A is thought to participate in the formation of the nacreous layer. Here we have identified a partial cDNA coding for the Lustrin A gene in H. tuberculata and have analysed its spatial and temporal expression during abalone development. RT-PCR experiments indicate that Lustrin A is first expressed in juvenile (post-metamorphosis) stages, suggesting that Lustrin A is a component of the juvenile shell, but not of the larval shell. We also detected Lustrin A mRNAs in non-nacre forming cells at the distal-most edge of the juvenile mantle as well as in the nacre-forming region of the mantle. Lustrin A was also expressed in 7-day-old post-larvae, prior to the formation of nacre. These results suggest that Lustrin A plays multiple roles in the shell-forming process and further highlight the dynamic ontogenic nature of molluscan shell formation.

}, keywords = {biomineralisation, Haliotis tuberculata, larval development, Lustrin A, mollusc, organic matrix, shell}, author = {Gaume, B{\'e}atrice and Denis, Fran{\c c}oise and Van Wormhoudt, Alain and Huchette, Sylvain and Jackson, Daniel and Avignon, Sol{\`e}ne and St{\'e}phanie Auzoux-Bordenave} } @article {4634, title = {In vitro effect of five pharmaceuticals on the viability of the European abalone hemocytes, Haliotis tuberculata}, journal = {J xenobiotics}, volume = {4}, year = {2014}, pages = {78-80}, keywords = {cell viability, Haliotis tuberculata, hemocyte, in vitro, pharmaceuticals}, doi = {10.4081/xeno.2014.4900}, author = {Letullier, Amandine and Minguez, Laetitia and Katherine Costil and Halm-Lemeille, Marie-Pierre and Jean-Marc Lebel and Antoine Serpentini} }