@article {6940,
	title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes- Application for Cartilage Engineering.},
	journal = {Mar Biotechnol (NY)},
	volume = {20},
	year = {2018},
	month = {2018 Aug},
	pages = {436-450},
	abstract = {<p>The shells of the bivalve mollusks are organo-mineral structures predominantly composed of calcium carbonate, but also of a minor organic matrix, a mixture of proteins, glycoproteins, and polysaccharides. These proteins are involved in mineral deposition and, more generally, in the spatial organization of the shell crystallites in well-defined microstructures. In this work, we extracted different organic shell extracts (acid-soluble matrix, acid-insoluble matrix, water-soluble matrix, guanidine HCl/EDTA-extracted matrix, referred as ASM, AIM, WSM, and EDTAM, respectively) from the shell of the scallop Pecten maximus and studied their biological activities on human articular chondrocytes (HACs). We found that these extracts differentially modulate the biological activities of HACs, depending on the type of extraction and the concentration used. Furthermore, we showed that, unlike ASM and AIM, WSM promotes maintenance of the chondrocyte phenotype in monolayer culture. WSM increased the expression of chondrocyte-specific markers (aggrecan and type II collagen), without enhancing that of the main chondrocyte dedifferentiation marker (type I collagen). We also demonstrated that WSM could favor redifferentiation of chondrocyte in collagen sponge scaffold in hypoxia. Thus, this study suggests that the organic matrix of Pecten maximus, particularly WSM, may contain interesting molecules with chondrogenic effects. Our research emphasizes the potential use of WSM of Pecten maximus for cell therapy of cartilage.</p>
},
	keywords = {Aged, Aged, 80 and over, Aggrecans, Animal Shells, Animals, Cell Differentiation, Cells, Cultured, Chondrocytes, Collagen Type II, Extracellular Matrix, Gene Expression Profiling, Humans, Middle Aged, Pecten, Phenotype},
	issn = {1436-2236},
	doi = {10.1007/s10126-018-9807-7},
	author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Antoine Serpentini and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence}
}
@article {8720,
	title = {Regulation of Extracellular Matrix Synthesis by Shell Extracts from the Marine Bivalve Pecten maximus in Human Articular Chondrocytes{\textemdash} Application for Cartilage Engineering},
	journal = {Marine Biotechnology},
	volume = {20},
	year = {2018},
	month = {Jan-08-2018},
	pages = {436 - 450},
	issn = {1436-2228},
	doi = {10.1007/s10126-018-9807-7},
	url = {http://link.springer.com/10.1007/s10126-018-9807-7},
	author = {Bouyoucef, Mouloud and Rakic, Rodolphe and G{\'o}mez-Leduc, Tangni and Latire, Thomas and Marin, Fr{\'e}d{\'e}ric and Leclercq, Sylvain and Carreiras, Franck and Serpentini, Antoine and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Legendre, Florence}
}
@article {4728,
	title = {Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa},
	journal = {Journal of The Royal Society Interface},
	volume = {14},
	year = {2017},
	abstract = {<p>The formation of the molluscan shell nacre is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell-forming tissue, the mantle. This so-called {\textquoteleft}calcifying matrix{\textquoteright} is a complex mixture of proteins, glycoproteins and polysaccharides that is assembled and occluded within the mineral phase during the calcification process. Better molecular-level characterization of the substances that regulate nacre formation is still required. Notable advances in expressed tag sequencing of freshwater mussels, such as Elliptio complanata and Villosa lienosa, provide a pre-requisite to further characterize bivalve nacre proteins by a proteomic approach. In this study, we have identified a total of 48 different proteins from the insoluble matrices of the nacre, 31 of which are common to both E. complanata and V. lienosa. A few of these proteins, such as PIF, MSI60, CA, shematrin-like, Kunitz-like, LamG, chitin-binding-containing proteins, together with A-, D-, G-, M- and Q-rich proteins, appear to be analogues, if not true homologues, of proteins previously described from the pearl oyster or the edible mussel nacre matrices, thus forming a remarkable list of deeply conserved nacre proteins. This work constitutes a comprehensive nacre proteomic study of non-pteriomorphid bivalves that has enabled us to describe the molecular basis of a deeply conserved biomineralization toolkit among nacreous shell-bearing bivalves, with regard to proteins associated with other shell microstructures, with those of other mollusc classes (gastropods, cephalopods) and, finally, with other lophotrochozoans (brachiopods).</p>
},
	issn = {1742-5689},
	doi = {10.1098/rsif.2016.0846},
	url = {http://rsif.royalsocietypublishing.org/content/14/126/20160846},
	author = {Marie, Benjamin and Arivalagan, Jaison and Math{\'e}ron, Lucr{\`e}ce and Bolbach, G{\'e}rard and Berland, Sophie and Marie, Arul and Marin, Fr{\'e}d{\'e}ric}
}
@article {8719,
	title = {Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro},
	journal = {Cytotechnology},
	volume = {69},
	year = {2017},
	month = {Jan-10-2017},
	pages = {815 - 829},
	issn = {0920-9069},
	doi = {10.1007/s10616-017-0096-1},
	url = {http://link.springer.com/10.1007/s10616-017-0096-1},
	author = {Latire, Thomas and Legendre, Florence and Bouyoucef, Mouloud and Marin, Fr{\'e}d{\'e}ric and Carreiras, Franck and Rigot-Jolivet, Muriel and Lebel, Jean-Marc and Gal{\'e}ra, Philippe and Serpentini, Antoine}
}
@inbook {4068,
	title = {Unveiling the evolution of bivalve nacre proteins by shell proteomics of Unionoidae. },
	booktitle = {Biomineralization: from fundamentals to biomaterials \& environmental issues},
	volume = {672},
	year = {2015},
	pages = {pp.158-167},
	edition = {Key Engineering Materials, 978-3-03835-591-5 Trans Tech Publications Ltd},
	chapter = {2},
	author = {Marie, Benjamin and Arivalagan, Jaison and Dubost, Lionel and Berland, Sophie and Marie, Arul and Marin, Fr{\'e}d{\'e}ric}
}
