%0 Journal Article %J Ecotoxicology %D 2023 %T Impact assessment of metals realeased by aluminium-based galvanic anode on the physiology of the abalone Haliotis tuberculata in controlled conditions %A Alexandre Levallois %A Nivelais, Laureen %A Christelle Caplat %A Jean-Marc Lebel %A Basuyaux, Olivier %A Katherine Costil %A Antoine Serpentini %X To protect metal structures immersed in the sea from corrosion, the galvanic anode cathodic protection system (GACP) is often applied. However, this association leads to continuous oxidation of the galvanic anode and therefore to a release of a metal cocktail in the forms of ions or oxy-hydroxides. Therefore, the main objective of our study was to investigate the toxicity of elements released from the dissolution of an aluminium-based galvanic anode (∼95% Al, ∼5% Zn, <0.1% for In, Cu, Cd, Mn, Fe) on a grazing gastropod, the abalone Haliotis tuberculata. The present study was carried out in complement to other research currently in submission. Gastropods were exposed for 16 weeks (12 weeks of exposure and 4 weeks of decontamination phase) to 6 conditions including a control, 4 concentrations based on total aluminium level (86, 425, 1096 and 3549 µg L−1 ) and a trophic control, corresponding to abalones placed in non-contaminated natural seawater but fed with contaminated algae. The effects of metals on growth, glycogen levels, brix index of hemolymph, MDA levels in digestive gland and gills, hemocyte phagocytic activity, ROS production, lysosomal system and the progress of gametogenesis were investigated throughout the entire exposure allowing the realization of kinetics. The results revealed that the aluminiumbased anode does not seem to have an effect on the health status of the individuals for environmentally realistic concentrations. However, in extreme conditions strong effects were reported on the growth, immune system and reproduction of abalone. %B Ecotoxicology %V 32 %P 438 - 450 %8 Jan-05-2023 %G eng %U https://link.springer.com/10.1007/s10646-023-02652-9 %N 4 %! Ecotoxicology %R 10.1007/s10646-023-02652-9 %0 Journal Article %J Aquatic Toxicology %D 2022 %T Effects of chronic exposure of metals released from the dissolution of an aluminium galvanic anode on the Pacific oyster Crassostrea gigas %A Alexandre Levallois %A Christelle Caplat %A Olivier Basuyaux %A Jean-Marc Lebel %A Antoine Laisney %A Katherine Costil %A Antoine Serpentini %K aluminium %K Bioaccumulation %K Biological effects %K Crassostrea gigas %K galvanic anode %K Oyster %X Among the anthropogenic sources releasing metallic species into the marine environment, the galvanic anode cathodic protection system (GACP) is widely used to protect submerged metallic structures from corrosion. Galvanic anodes are an alloy of metals of which the main component is aluminum or zinc. Very few studies were performed to study their potential biological effects. We investigated the chronic toxicity of an aluminum-based galvanic anode on the Pacific oyster, Crassostrea gigas. Oysters were exposed for 84 days to three concentrations of aluminum (50, 100 and 300 µg L−1) obtained with an electrochemical experimental device simulating the dissolution of a galvanic anode. At different exposure times, we studied a battery of biomarkers of the immune system, reproductive parameters and the metabolic state of the oysters. Results demonstrated a sensitivity of oysters at the highest concentration and some biological effects were observed especially for the malondialdehyde content in the digestive gland after 84 days of exposure. In addition to these biomarkers, the bioaccumulation of the different metals composing the anode was measured in oysters’ tissues. Bivalves bioaccumulated more zinc than aluminum, even if aluminium was present in greater concentrations during exposures. Moreover, exposure time did not influence the bioaccumulation of aluminum in contrast to zinc. %B Aquatic Toxicology %V Volume 249 %G eng %R https://doi.org/10.1016/j.aquatox.2022.106223 %0 Journal Article %J Environ Sci Pollut Res Int %D 2016 %T Sub-chronic exposure to fluoxetine in juvenile oysters (Crassostrea gigas): uptake and biological effects. %A Di Poi, C %A L Evariste %A Séguin, Alexis %A Mottier, A %A Pedelucq, Julie %A Jean-Marc Lebel %A Antoine Serpentini %A Budzinski, Hélène %A Katherine Costil %X
The bioconcentration potential of fluoxetine (FLX) and its biological effects were investigated in juvenile Pacific oyster exposed for 28 days to environmentally relevant concentrations of FLX (1 ng L(-1), 100 ng L(-1) and up to 10 μg L(-1)). FLX bioaccumulated in oyster flesh resulting in 28-day bioconcentration factors greater than 2,000 and 10,000 by referring to wet and dry weights, respectively. Nevertheless, FLX did not induce oyster mortality, delayed gametogenesis, or lead to adverse histopathological alterations. At the two highest concentrations, despite non-optimal trophic conditions, FLX stimulated shell growth but only in a transient manner, suggesting a role of serotonin in the regulation of feeding and metabolism in bivalves. Those high concentrations seemed to drive bell-shaped responses of catalase and glutathione S-transferase activities throughout the exposure period, which may indicate the activation of antioxidant enzyme synthesis and then an enhanced catabolic rate or direct inhibition of those enzymes. However, no clear oxidative stress was detected because no strong differences in thiobarbituric acid-reactive substance (TBARS) content (i.e. lipid peroxidation) were observed between oyster groups, suggesting that cellular defence mechanisms were effective. These results demonstrate the importance of considering additional biomarkers of oxidative stress to obtain a comprehensive overview of the FLX-induced changes in marine bivalves exposed under realistic conditions. Considering the battery of biomarkers used, FLX appears to induce little or no effects on oyster physiology even at a concentration of 10 μg L(-1). These results do not confirm the lowest observed effect concentration (LOEC) values reported by some authors in other mollusc species.
%B Environ Sci Pollut Res Int %8 2014 Oct 15 %G eng %R 10.1007/s11356-014-3702-1 %0 Journal Article %J Environ Sci Pollut Res Int %D 2015 %T Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata? %A Ladhar-Chaabouni, R %A Machreki-Ajmi, Monia %A Antoine Serpentini %A Jean-Marc Lebel %A Hamza-Chaffai, A %XIn this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24 h to CdCl2 concentrations of 0, 1 and 100 μg ml(-1). The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1 μg ml(-1) of CdCl2.
%B Environ Sci Pollut Res Int %8 2014 Aug 19 %G eng %R 10.1007/s11356-014-3387-5 %0 Journal Article %J Chemosphere %D 2014 %T Acute toxicity of 8 antidepressants: what are their modes of action? %A Minguez, Laetitia %A Farcy, E %A Ballandonne, Céline %A Lepailleur, Alban %A Antoine Serpentini %A Jean-Marc Lebel %A Bureau, Ronan %A Halm-Lemeille, Marie-Pierre %K Animals %K Antidepressive Agents %K Cell Survival %K Cells, Cultured %K Daphnia %K Environmental Pollutants %K Gastropoda %K Hemocytes %K Lysosomes %K Models, Molecular %K Phosphatidylcholines %K Serotonin Uptake Inhibitors %XCurrently, the hazard posed by pharmaceutical residues is a major concern of ecotoxicology. Most of the antidepressants belong to a family named the Cationic Amphipathic Drugs known to have specific interactions with cell membranes. The present study assessed the impact of eight antidepressants belonging to selective serotonin reuptake inhibitors or serotonin norepinephrine reuptake inhibitors by the combination of multi-approaches (in vivo, in vitro, in silico) and gives some insights on the mode of action for these molecules. Antidepressants were from the most to the least toxic compound for Daphnia magna: Sertraline (EC50=1.15 mg L(-1))>Clomipramine (2.74 mg L(-1))>Amitriptyline (4.82 mg L(-1))>Fluoxetine (5.91 mg L(-1))>Paroxetine (6.24 mg L(-1))>Mianserine (7.81 mg L(-1))>Citalopram (30.14 mg L(-1)) and Venlafaxine (141.28 mg L(-1)). These acute toxicities were found correlated to Log Kow coefficients (R=0.93, p<0.001) and to cytotoxicity assessed on abalone hemocytes through the neutral red uptake assay (R=0.96, p<0.001). If narcosis as mode of action is typically expected during acute ecotoxicity bioassays, we showed by molecular modeling that particular interactions can exist between antidepressants and phosphatidylcholine, a major component of cell membranes, leading to a more specific mode of action corresponding to a potential acidic hydrolysis of ester functions.
%B Chemosphere %V 108 %P 314-9 %8 2014 Aug %G eng %R 10.1016/j.chemosphere.2014.01.057 %0 Journal Article %J Aquat Toxicol %D 2014 %T Assessment of cytotoxic and immunomodulatory properties of four antidepressants on primary cultures of abalone hemocytes (Haliotis tuberculata). %A Minguez, Laetitia %A Halm-Lemeille, Marie-Pierre %A Katherine Costil %A Bureau, Ronan %A Jean-Marc Lebel %A Antoine Serpentini %K Animals %K Antidepressive Agents %K Cell Survival %K Cells, Cultured %K Enzyme Activation %K Esterases %K Gastropoda %K Hemocytes %K Immunity, Innate %K Lethal Dose 50 %K Phagocytosis %K Reactive Oxygen Species %K Water Pollutants, Chemical %XPharmaceutical compounds like antidepressants found in surface waters raise concerns due to their potential toxicity on non-target aquatic organisms. This study aimed at investigating the in vitro cytotoxicity and immunomodulatory properties of four common antidepressants, namely Amitriptyline, Clomipramine, Citalopram and Paroxetine, on primary cultures of abalone hemocytes (Haliotis tuberculata), after 48 h-exposure. Effects on immunocompetence (phagocytosis, levels of reactive oxygen species, esterase activity and lysosomal membrane destabilization) were assessed. Results obtained by MTT assays revealed that acute toxicity is unlikely to occur in the environment since the LC50s of the four antidepressants are at the mg/L level. The different immunological endpoints displayed a biphasic response, with an increase at the lowest concentration (i.e. 1 μg/L) followed by a decrease at higher concentrations. Overall, Amitriptyline and Clomipramine, the two tricyclic antidepressants, had higher immunomodulatory capacities than the two selective serotonin reuptake inhibitors Citalopram and Paroxetine. Amitriptyline was the most potent and Citalopram the least potent drug in altering immune function in H. tuberculata.
%B Aquat Toxicol %V 153 %P 3-11 %8 2014 Aug %G eng %R 10.1016/j.aquatox.2013.10.020 %0 Journal Article %J Ecotoxicology %D 2014 %T Comparison of the sensitivity of seven marine and freshwater bioassays as regards antidepressant toxicity assessment. %A Minguez, Laetitia %A Di Poi, C %A Farcy, E %A Ballandonne, Céline %A Benchouala, Amira %A Bojic, Clément %A Cossu-Leguille, Carole %A Katherine Costil %A Antoine Serpentini %A Jean-Marc Lebel %A Halm-Lemeille, Marie-Pierre %XThe hazards linked to pharmaceutical residues like antidepressants are currently a major concern of ecotoxicology because they may have adverse effects on non-target aquatic organisms. Our study assesses the ecotoxicity of three antidepressants (fluoxetine, sertraline and clomipramine) using a battery of marine and freshwater species representing different trophic levels, and compares the bioassay sensitivity levels. We selected the following bioassays: the algal growth inhibition test (Skeletonema marinoi and Pseudokirchneriella subcapitata), the microcrustacean immobilization test (Artemia salina and Daphnia magna), development and adult survival tests on Hydra attenuata, embryotoxicity and metamorphosis tests on Crassostrea gigas, and in vitro assays on primary cultures of Haliotis tuberculata hemocytes. The results showed high inter-species variability in EC50-values ranging from 43 to 15,600 µg/L for fluoxetine, from 67 to 4,400 µg/L for sertraline, and from 4.70 µg/L to more than 100,000 µg/L for clomipramine. Algae (S. marinoi and P. subcapitata) and the embryo-larval stages of the oyster C. gigas were the most sensitive taxa. This raises an issue due to their ecological and/or economic importance. The marine crustacean A. salina was the least sensitive species. This difference in sensitivity between bioassays highlights the importance of using a test battery.
%B Ecotoxicology %V 23 %P 1744-54 %8 2014 Nov %G eng %N 9 %R 10.1007/s10646-014-1339-y %0 Journal Article %J Chemosphere %D 2014 %T The effect of different polychlorinated biphenyls on two aquatic models, the green alga Pseudokirchneriella subcapitata and the haemocytes from the European abalone Haliotis tuberculata. %A Halm-Lemeille, Marie-Pierre %A Abbaszadeh Fard, Elham %A Latire, T %A Ferard, Jean-François %A Katherine Costil %A Jean-Marc Lebel %A Bureau, Ronan %A Antoine Serpentini %K Animals %K Chlorophyta %K Environmental Pollutants %K Gastropoda %K Hemocytes %K Polychlorinated Biphenyls %K Tetrachlorodibenzodioxin %XThe present study was conducted to determine the toxicity of different polychlorinated biphenyls (PCBs) on the green algae, Pseudokirchneriella subcapitata and the haemocytes from the European abalone, Haliotis tuberculata. Using the algal growth inhibition test, the green algae median Effective Concentration (EC50) values ranged from 0.34μM for PCB28 to more than 100μM for PCBs 101 and 153. Considering the MTT viability test, the abalone EC50 values ranged from 1.67μM for PCB153 to 89μM for PCB28. Our results in contrast to previous observation in vertebrates did not show significant differences between the dioxin like- and non dioxin like-PCBs toxicities regardless of the model used. However, our results demonstrated that the toxicities of PCBs were species dependent. For example, PCB28 was the most toxic compound for P. subcapitata whereas PCBs 1, 180 and 153 were less toxic for that species. On the contrary, PCB153 was reported as the most toxic for H. tuberculata haemocytes and PCB28 the least toxic. To investigate the mode of action of these compounds, we used an in silico method. Our results suggested that PCBs have a non-specific mode of action (e.g., narcosis) on green algae, and another mode of action, probably more specific than narcosis, was reported for PCBs on the abalone haemocytes.
%B Chemosphere %V 110 %P 120-8 %8 2014 Sep %G eng %R 10.1016/j.chemosphere.2014.02.023 %0 Journal Article %J Aquat Toxicol %D 2014 %T Effects of acute exposures to mecoprop, mecoprop-p and their biodegradation product (2-MCP) on the larval stages of the Pacific oyster, Crassostrea gigas. %A Mottier, A %A Kientz-Bouchart, Valérie %A Dubreule, Christelle %A Antoine Serpentini %A Jean-Marc Lebel %A Katherine Costil %K 2-Methyl-4-chlorophenoxyacetic Acid %K Animals %K Chlorophenols %K Crassostrea %K Embryo, Nonmammalian %K Larva %K Metamorphosis, Biological %K Water Pollutants, Chemical %XStudies have shown that pesticides are sometimes detected at rather high levels in seawater and it has been suggested that these chemical compounds could act as additional stress factor for oysters cultured in coastal environments. The effects of pesticides on marine molluscs could be particularly harmful in the early stages which correspond to critical life stages. This study aimed to assess the effects of mecoprop, mecoprop-p and their degradation compound 2-methyl-4-chlorophenol on two larval stages of Crassostrea gigas. Embryotoxic effects were assessed on veliger larvae after 36 h exposures, and both percentages of normal larvae and types of abnormalities were taken into account. The effects of the three substances were evaluated on 21-day-old pediveliger larvae by calculating metamorphosis rates after 24h exposures. The results of the embryotoxicity assay indicated that 2-methyl-4-chlorophenol was more toxic (EC50: 10.81 mg L(-1)) than its parent compounds (EC50 mecoprop: 42.55 mg L(-1); EC50 mecoprop-p: 78.85 mg L(-1)). Mecoprop in particular injured shell formation with an increase of shell abnormalities following herbicide concentrations. The active substances were not toxic to metamorphosis processes, but 2-MCP was revealed to be more toxic to the success of metamorphosis (EC50: 7.20 mg L(-1)) than to embryo-larval development. However, the toxic concentrations were several orders of magnitude higher than environmental concentrations.
%B Aquat Toxicol %V 146 %P 165-75 %8 2014 Jan %G eng %R 10.1016/j.aquatox.2013.11.008 %0 Journal Article %J Fish Shellfish Immunol %D 2014 %T Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis). %A Le Pabic, C %A Goux, Didier %A Guillamin, Maryline %A Safi, Georges %A Jean-Marc Lebel %A Siméoni Koueta-Noussithé %A Antoine Serpentini %XLittle is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.
%B Fish Shellfish Immunol %V 40 %P 362-73 %8 2014 Oct %G eng %N 2 %R 10.1016/j.fsi.2014.07.020 %0 Journal Article %J J xenobiotics %D 2014 %T In vitro effect of five pharmaceuticals on the viability of the European abalone hemocytes, Haliotis tuberculata %A Letullier, Amandine %A Minguez, Laetitia %A Katherine Costil %A Halm-Lemeille, Marie-Pierre %A Jean-Marc Lebel %A Antoine Serpentini %K cell viability %K Haliotis tuberculata %K hemocyte %K in vitro %K pharmaceuticals %B J xenobiotics %V 4 %P 78-80 %G eng %N 2 %R 10.4081/xeno.2014.4900 %0 Journal Article %D 2014 %T Prophenoloxidase system, lysozyme and protease inhibitor distribution in the common cuttlefish Sepia officinalis %A Le Pabic, C %A Safi, Georges %A Antoine Serpentini %A Jean-Marc Lebel %A Jean-Paul Robin %A Siméoni Koueta-Noussithé %G eng %0 Journal Article %J PLoS One %D 2014 %T Shell extracts from the marine bivalve Pecten maximus regulate the synthesis of extracellular matrix in primary cultured human skin fibroblasts. %A Latire, T %A Legendre, Florence %A Bigot, Nicolas %A Carduner, Ludovic %A Kellouche, Sabrina %A Bouyoucef, Mouloud %A Carreiras, Franck %A Marin, F %A Jean-Marc Lebel %A Galéra, Philippe %A Antoine Serpentini %XMollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the -112/-61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.
%B PLoS One %V 9 %P e99931 %8 2014 %G eng %N 6 %R 10.1371/journal.pone.0099931