%0 Journal Article %J PLoS One %D 2014 %T Shell extracts from the marine bivalve Pecten maximus regulate the synthesis of extracellular matrix in primary cultured human skin fibroblasts. %A Latire, T %A Legendre, Florence %A Bigot, Nicolas %A Carduner, Ludovic %A Kellouche, Sabrina %A Bouyoucef, Mouloud %A Carreiras, Franck %A Marin, F %A Jean-Marc Lebel %A Galéra, Philippe %A Antoine Serpentini %X

Mollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the -112/-61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.

%B PLoS One %V 9 %P e99931 %8 2014 %G eng %N 6 %R 10.1371/journal.pone.0099931 %0 Journal Article %J PLoS One %D 2014 %T The skeleton of the staghorn coral Acropora millepora: molecular and structural characterization. %A Ramos-Silva, Paula %A Kaandorp, Jaap %A Herbst, Frédéric %A Plasseraud, Laurent %A Alcaraz, G %A Stern, Christine %A Corneillat, Marion %A Guichard, N %A Durlet, Christophe %A Gilles Luquet %A Marin, F %K Acetic Acid %K Amination %K Animals %K Anthozoa %K Bone and Bones %K Calcium Carbonate %K Crystallization %K Gels %K Monosaccharides %K Proteins %K Solubility %K Spectroscopy, Fourier Transform Infrared %K Spectrum Analysis, Raman %X

The scleractinian coral Acropora millepora is one of the most studied species from the Great Barrier Reef. This species has been used to understand evolutionary, immune and developmental processes in cnidarians. It has also been subject of several ecological studies in order to elucidate reef responses to environmental changes such as temperature rise and ocean acidification (OA). In these contexts, several nucleic acid resources were made available. When combined to a recent proteomic analysis of the coral skeletal organic matrix (SOM), they enabled the identification of several skeletal matrix proteins, making A. millepora into an emerging model for biomineralization studies. Here we describe the skeletal microstructure of A. millepora skeleton, together with a functional and biochemical characterization of its occluded SOM that focuses on the protein and saccharidic moieties. The skeletal matrix proteins show a large range of isoelectric points, compositional patterns and signatures. Besides secreted proteins, there are a significant number of proteins with membrane attachment sites such as transmembrane domains and GPI anchors as well as proteins with integrin binding sites. These features show that the skeletal proteins must have strong adhesion properties in order to function in the calcifying space. Moreover this data suggest a molecular connection between the calcifying epithelium and the skeletal tissue during biocalcification. In terms of sugar moieties, the enrichment of the SOM in arabinose is striking, and the monosaccharide composition exhibits the same signature as that of mucus of acroporid corals. Finally, we observe that the interaction of the acetic acid soluble SOM on the morphology of in vitro grown CaCO3 crystals is very pronounced when compared with the calcifying matrices of some mollusks. In light of these results, we wish to commend Acropora millepora as a model for biocalcification studies in scleractinians, from molecular and structural viewpoints.

%B PLoS One %V 9 %P e97454 %8 2014 %G eng %N 6 %R 10.1371/journal.pone.0097454