%0 Journal Article %J Deep Sea Research Part II: Topical Studies in Oceanography %D 2015 %T Behavioural study of two hydrothermal crustacean decapods: Mirocaris fortunata and Segonzacia mesatlantica, from the Lucky Strike vent field (Mid-Atlantic Ridge) %A Matabos Marjolaine %A Cuvelier Daphné %A Brouard Johan %A Bruce Shillito %A Juliette Ravaux %A Magali Zbinden %A Barthelemy Dominique %A Sarradin, Pierre-Marie %A Sarrazin, Jozee %K 32°16.3′W %K 37°17′N %K AbyssBox %K Biological interactions %K Deep-sea observatory %K Eiffel Tower edifice %K Experimental research %K Feeding behaviour %K Lucky Strike %K Mid-Atlantic Ridge %K Time series %K Video imagery %X Identifying the factors driving community dynamics in hydrothermal vent communities, and in particular biological interactions, is challenged by our ability to make direct observations and the difficulty to conduct experiments in those remote ecosystems. As a result, we have very limited knowledge on species׳ behaviour and interactions in these communities and how they in turn influence community dynamics. Interactions such as competition or predation significantly affect community structure in vent communities, and video time-series have successfully been used to gain insights in biological interactions and species behaviour, including responses to short-term changes in temperature or feeding strategies. In this study, we combined in situ and ex situ approaches to characterise the behaviour and interactions among two key species encountered along the Mid-Atlantic Ridge (MAR): the shrimp Mirocaris fortunata and the crab Segonzacia mesatlantica. In situ, species small-scale distribution, interactions and behaviour were studied using the TEMPO observatory module deployed on the seafloor at the base of the active Eiffel Tower edifice in the Lucky Strike vent field as part of the EMSO-Açores MoMAR observatory. TEMPO sampled 2min of video four times a day from July 2011 to April 2012. One week of observations per month was used for ‘long-term’ variations, and a full video data set was analysed for January 2012. In addition, observations of crab and shrimp individuals maintained for the first time under controlled conditions in atmospheric pressure (classic tank) and pressurised (AbyssBox) aquaria allowed better characterisation and description of the different types of behaviour and interactions observed in nature. While the identified in situ spatial distribution pattern was stable over the nine months, both species displayed a significant preference for mussel bed and anhydrite substrata, and preferentially occupied the area located directly in the fluid flow axis. The aggregation behaviour of M. fortunata resulted in the occurrence of numerous intraspecific interactions mainly involving the use of two pairs of sensory organs (antenna/antennule) and fleeing behaviours when in contact or close to individuals of S. mesatlantica. The higher level of passiveness observed in the ex situ artificial environment compared to the in situ environment was attributed to the lack of stimulation related to low densities of congeners and/or of sympatric species compared to the natural environment and the absence of continuous food supply, as both species displayed a significant higher level of activity during feeding time. This result emphasises the role of food supply as a driver of species distribution and behaviour. Direct in situ observations using cameras deployed on deep-sea observatories, combined with experimental set-up in pressurised aquaria, will help investigators understand the factors influencing community dynamics and species biology at vents as well as their underlying mechanisms. %B Deep Sea Research Part II: Topical Studies in Oceanography %V 121 %P 146 - 158 %G eng %U http://www.sciencedirect.com/science/article/pii/S0967064515001113 %R https://doi.org/10.1016/j.dsr2.2015.04.008 %0 Journal Article %J Deep Sea Research Part II: Topical Studies in Oceanography %D 2015 %T Long-term maintenance and public exhibition of deep-sea hydrothermal fauna: The AbyssBox project %A Bruce Shillito %A Juliette Ravaux %A Sarrazin, Jozee %A Zbinden, M. %A Sarradin, Pierre-Marie %A Barthelemy, D. %B Deep Sea Research Part II: Topical Studies in Oceanography %V 121 %P 137 - 145 %8 Jan-11-2015 %G eng %U https://linkinghub.elsevier.com/retrieve/pii/S0967064515001460 %! Deep Sea Research Part II: Topical Studies in Oceanography %R 10.1016/j.dsr2.2015.05.002 %0 Journal Article %J Deep Sea Research Part II: Topical Studies in Oceanography %D 2015 %T Long-term maintenance and public exhibition of deep-sea hydrothermal fauna: The AbyssBox project %A Bruce Shillito %A Juliette Ravaux %A Sarrazin, Jozee %A Magali Zbinden %A Sarradin, Pierre-Marie %A Barthelemy Dominique %K AbyssBox %K Aquaria %K Aquariology %K Deep waters %K Hydrostatic Pressure %K Hydrothermal Vents %K Public exhibition %K Scientific experiments %X The AbyssBox project aims to provide the first permanent public exhibition of live deep-sea hydrothermal fauna maintained at in situ pressure. AbyssBox is a pressurized aquarium designed to function permanently. Here we present details of the project after the public exhibition functioned for more than three years at Océanopolis aquarium in Brest, France. We also describe the AbyssBox pressure aquarium, and provide data and observations on vent shrimp (Mirocaris fortunata) and crabs (Segonzacia mesatlantica) that were sampled from 1700m depth at the Lucky Strike vent field (Mid-Atlantic Ridge) during different cruises. While mortalities exceeded 50% during the first days following sampling, the remaining animals appeared to acclimate fairly well. Some crabs have now been kept for more than 2 years, and some shrimp have spent more than 3 years in captivity. Primarily designed for a public exhibition, the AbyssBox is already used for scientific purposes, since it provides one of the most effective tools for long-term rearing of deep-sea fauna. AbyssBox is a first step towards maintaining a variety of deep-sea fauna year-round at in situ pressure, which will serve both scientific and public interests. %B Deep Sea Research Part II: Topical Studies in Oceanography %V 121 %P 137 - 145 %G eng %U http://www.sciencedirect.com/science/article/pii/S0967064515001460 %R https://doi.org/10.1016/j.dsr2.2015.05.002