%0 Journal Article %J Marine Drugs %D 2022 %T Structural and Functional Characterization of Orcokinin B-like Neuropeptides in the Cuttlefish (Sepia officinalis) %A Endress, Maxime %A Céline Zatylny-Gaudin %A Leprince, Jérôme %A Lefranc, Benjamin %A Corre, Erwan %A Le Corguillé, Gildas %A Bernay, Benoît %A Leduc, Alexandre %A Rangama, Jimmy %A Mouret, Liza %A Lafont, Anne-Gaelle %A Bondon, Arnaud %A Joël Henry %B Marine Drugs %V 20 %P 505 %8 Jan-08-2022 %G eng %U https://www.mdpi.com/1660-3397/20/8/505 %N 8 %! Marine Drugs %R 10.3390/md20080505 %0 Journal Article %J The Journal of Experimental Biology %D 2019 %T Characterization of an evolutionarily conserved calcitonin signalling system in a lophotrochozoan, the Pacific oyster (Crassostrea gigas) %A Julie Schwartz %A Réalis-Doyelle, Emilie %A Marie-Pierre Dubos %A Lefranc, Benjamin %A Leprince, Jérôme %A Pascal Favrel %B The Journal of Experimental Biology %V 222 %P jeb201319 %8 Jan-07-2019 %G eng %U http://jeb.biologists.org/lookup/doi/10.1242/jeb.201319 %N 13 %! J Exp Biol %R 10.1242/jeb.201319 %0 Journal Article %J J Exp Biol %D 2019 %T Characterization of an evolutionarily conserved calcitonin signalling system in a lophotrochozoan, the Pacific oyster (). %A Julie Schwartz %A Réalis-Doyelle, Emilie %A Marie-Pierre Dubos %A Lefranc, Benjamin %A Leprince, Jérôme %A Pascal Favrel %X

In Protostoma, the diuretic hormone 31 (DH31) signalling system was long considered as the orthologue of the chordate calcitonin (CT) signalling system. Using the Pacific oyster () transcriptomic database GigaTON, we characterized seven G-protein-coupled receptors (GPCRs) named Cragi-CTR1-7 and phylogenetically related to chordate CT receptors (CTRs) and to protostome DH31 receptors. Two CT precursors (Cragi-CTP1 and Cragi-CTP2) containing two CT-type peptides and encoded by two distinct genes with a similar organization were also characterized. These oyster neuropeptides (Cragi-CT1/2) exhibit the two N-terminal paired cysteine residues and, except CTP2-derived peptide (Cragi-CTP2dp), show the C-terminal proline-amide motif typical of deuterostome CT-type peptides. All mature Cragi-CTs except Cragi-CTP2dp were detected in visceral ganglion extracts using mass spectrometry. Cell-based assays revealed that the previously characterized oyster receptors Cg-CT-R and Cragi-CTR2 were specifically activated by Cragi-CT1b and Cragi-CT2, respectively. This activation does not require the co-expression of receptor activity-modifying proteins (RAMPs). Thus, oyster CT signalling appears functionally more closely related to vertebrate CT/CTR signalling than to calcitonin gene-related peptide/calcitonin receptor-like receptor (CGRP/CLR) signalling. Gene expression profiles in different adult tissues and in oysters acclimated to brackish water suggest the potential implication of both Cg-CT-R/Cragi-CT1b and Cragi-CTR2/Cragi-CT2 in water and ionic regulations, although with apparently opposite effects. The present study represents the first comprehensive characterization of a functional CT-type signalling system in a protostome and provides evidence for its evolutionarily ancient origin and its early role in osmotic homeostasis.

%B J Exp Biol %V 222 %8 2019 Jul 05 %G eng %N Pt 13 %R 10.1242/jeb.201319 %0 Journal Article %J Gen. Comp. Endocrinol. %D 2018 %T Crustacean cardioactive peptides: Expression, localization, structure, and a possible involvement in regulation of egg-laying in the cuttlefish Sepia officinalis. %A Endress, Maxime %A Céline Zatylny-Gaudin %A Corre, Erwan %A Le Corguillé, Gildas %A Benoist, Louis %A Leprince, Jérôme %A Lefranc, Benjamin %A Bernay, Benoît %A Leduc, Alexandre %A Rangama, Jimmy %A Anne-Gaelle Lafont %A Bondon, Arnaud %A Joël Henry %X

The cuttlefish (Sepia officinalis) is a cephalopod mollusk distributed on the western European coast, in the West African Ocean and in the Mediterranean Sea. On the Normandy coast (France), cuttlefish is a target species of professional fishermen, so its reproduction strategy is of particular interest in the context of stock management. Egg-laying, which is coastal, is controlled by several types of regulators among which neuropeptides. The cuttlefish neuropeptidome was recently identified by Zatylny-Gaudin et al. (2016). Among the 38 neuropeptide families identified, some were significantly overexpressed in egg-laying females as compared to mature males. This study is focused on crustacean cardioactive peptides (CCAPs), a highly expressed neuropeptide family strongly suspected of being involved in the control of egg-laying. We investigated the functional and structural characterization and tissue mapping of CCAPs, as well as the expression patterns of their receptors. CCAPs appeared to be involved in oocyte transport through the oviduct and in mechanical secretion of capsular products. Immunocytochemistry revealed that the neuropeptides were localized throughout the central nervous system (CNS) and in the nerve endings of the glands involved in egg-capsule synthesis and secretion, i.e. the oviduct gland and the main nidamental glands. The CCAP receptor was expressed in these glands and in the subesophageal mass of the CNS. Multiple sequence alignments revealed a high level of conservation of CCAP protein precursors in Sepia officinalis and Loligo pealei, two cephalopod decapods. Primary sequences of CCAPs from the two species were fully conserved, and cryptic peptides detected in the nerve endings were also partially conserved, suggesting biological activity that remains unknown for the time being.

%B Gen. Comp. Endocrinol. %V 1 %P 67-79 %G eng %N 260 %R 10.1016/j.ygcen.2017.12.009 %0 Journal Article %J Frontiers in Endocrinology %D 2018 %T Tachykinin-3 Genes and Peptides Characterized in a Basal Teleost, the European Eel: Evolutionary Perspective and Pituitary Role. %A Campo, Aurora %A Anne-Gaelle Lafont %A Lefranc, Benjamin %A Leprince, Jérôme %A Tostivint, Hervé %A Kamech, Nedia %A Sylvie Dufour %A Karine Rousseau %B Frontiers in Endocrinology %V 9 %P 304 %G eng