%0 Journal Article %J Scientific Data %D 2020 %T A macroscopic and stereological imaging dataset of Pleuronectes platessa ovariesAbstract %A Sauger, Carine %A Quinquis, Jérôme %A Kellner, Kristell %A Clothilde Heude-Berthelin %A Lepoittevin, Mélanie %A Elie, Nicolas %A Dubroca, Laurent %B Scientific Data %V 7 %8 Jan-12-2020 %G eng %U http://www.nature.com/articles/s41597-020-0505-8 %N 1 %! Sci Data %R 10.1038/s41597-020-0505-8 %0 Journal Article %J Journal of Molluscan Studies %D 2016 %T Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve, Pecten maximus (Linnaeus, 1758) %A Boonmee, A %A Clothilde Heude-Berthelin %A Kingtong, S %A Pauletto, M %A Bernay, B %A Adeline, B %A Suquet, M %A Pascal Sourdaine %A Kristell Kellner-Cousin %B Journal of Molluscan Studies %P 1-10 %8 09/2016 %G eng %0 Journal Article %J Environ Sci Pollut Res Int %D 2016 %T Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. %A Akcha, F %A Barranger, A %A Bachère, E %A Clothilde Heude-Berthelin %A Piquemal, D %A Alonso, P %A Sallan, R Rondon %A Dimastrogiovanni, G %A Porte, C %A Menard, D %A Szczybelski, A %A Benabdelmouna, A %A Auffret, M %A Rouxel, J %A Burgeot, T %X

Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA.

%B Environ Sci Pollut Res Int %V 23 %P 8008-20 %8 2016 Apr %G eng %N 8 %R 10.1007/s11356-015-5969-2 %0 Journal Article %J Comp Biochem Physiol C Toxicol Pharmacol %D 2015 %T Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. %A Barranger, Audrey %A Clothilde Heude-Berthelin %A Rouxel, Julien %A Adeline, Béatrice %A Benabdelmouna, Abdellah %A Burgeot, Thierry %A Akcha, Farida %X

Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.

%B Comp Biochem Physiol C Toxicol Pharmacol %8 2015 Nov 21 %G eng %R 10.1016/j.cbpc.2015.11.002