The spermatogonial stem cells niche is well studied in Osteichthyes (mammals, fish…) with relatively well conserved cellular markers and regulation pathways. In contrast, these aspects are rarely documented in Chondrichthyes, the Osteichthyes sister group, whose one quarter of the species are threatened due to over-fishing. So, we chose the small spotted catshark, Scyliorhinus canicula, an abundant species on our coasts, to explore the spermatogonial stem cells regulation in sharks and to compare it to other Vertebrates. Cells of the niche are characterized at a molecular level and the in vitro maintenance of these cells allows now the functional analysis of paracrine factors involved in the spermatogonial stem cells niche.
Key words: biology of reproduction, spermatogonial stem cells, paracrines factors, small spotted catshark
The japanese oyster, Crassostrea gigas, is a well-studied species in our team due to its aquaculture interest. The comprehension of the molecular mechanisms underlying its development and various physiological processes is however limited by the lack of tools for in vivo functional analysis. We develop gene invalidation in this species by a CRISPR/Cas9 approach.
Key words: biology of development, in vivo functional analysis, japanese oyster
Teaching activities at IBFA
In Bachelor’s degree in biology (L1-3): Animal Biology, Cellular Biology of Eukaryotes, Structure and Functioning in the Animal Kingdom
In second year of the Professional Master AQUACaen: Knowledge of the Socio-professional Environment, Fishing and Fish Breeding
University diploma: Animal experimentation applied to the Biomedical Research (aquatic animals)
Knowledge diffusion to general public
Participation in the science festival at Caen