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Marie-Pierre DUBOS

Marie-Pierre DUBOS's picture

Position

Engineer

Affiliation

Université de CAEN Normandie (UCN)

Email

marie-pierre.dubos@unicaen.fr

Phone

+ 33 2 31 56 57 90

Fax

+33 2 31 56 53 46

Localisation

Université de Caen Normandie (UCN)

Team

Team 2: Reproduction and development of aquatic organisms: evolution, adaptation, regulations
PCR quantitative

Research topics

  • Main Theme:

Cloning and characterization of G-protein-coupled-receptors and identification of their endogenous ligands in Pacific oyster Crassostrea gigas

  • Secondary Themes:

Involvement in the other themes of team 2 according to needs.

  • Tools:
    • Techniques in molecular biology: DNA and RNA extraction, real time quantitative PCR, PCR, Electrophoresis, Sub cloning, Purification of plasmids …
    • Techniques in cell culture: Primary culture and maintenance of cell lines, Transfection …
    • Biochemical techniques: Extraction of various cellular constituents (proteins, lipids), Reverse Endocrinology, Biochemical assays with Spectrometer and Luminometer …
    • Techniques in histology: Sample preparation, Tissue section with microtome and cryostat, Staining, Immunohistochemistry, In situ hybridization, Laser microdissection.

Teaching

  • Transfert of skills with the students in Master's degree and in thesis :

Training to the techniques of molecular biology, cell culture and biochemistry …

  • Broadcasting of knowledge with the general public:

Participation to the science Festival at Caen

Administrative responsibilities

  • Assistant of Prevention appointed since 2007
  • Member of council of laboratory caennais (Representative security) appointed since 2008
  • Member of council of the UMR BOREA (Representative of the staff) elected for period 2014-2018

Publications

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