- Kristell KELLNER
My main topics concern physiology of reproduction of various molluscan species with a focus on the bivalve Crassostrea gigas:
Investigate the reproduction of several species
Gametogenesis in Crassostrea gigas was described for diploid and triploid animals. Indeed, the possibility for triploid to produce mature gametes is of interest because of the potential impact of these animals in natural environment and mainly on diploid oysters populations.
The reproduction of the gastropod mollusc Buccinum undatum in various locations and according to size is also investigated in order to confirm compatibility between minimal fishing size and stock renewing. The impact of temperature on gametogenesis, spawning and development is of interrest in the context of global changes.
Simultaneous management of glycogen storage and reproduction
In Crassostrea gigas reserves are mainly constituted by glycogen. They sustained the reproductive effort of animals. Specialized glycogen cells have been characterised from histological and metabolic point of view (glucose entrance, glycogen synthesis). The interactions between glycogen reserves and reproduction were also described using controlled conditioning experiments.
Characterisation and fonctions of the insulin system
Effectors of the insulin pathway are involved in the energetic balance Crassostrea gigas. The Insulin receptor has been identified and its expression has been investigated in various organs. Several effectors of the insulin pathway have also been investigated.
Characterisation of the neurosecretory system in bivalve species
Metabolic pathway in molluscs also involved internal regulatory mecanisms. The nervous system is the main centre of neurohormones production. Structure and functioning of neurosecretory cells have to be characterized in bivalvia (main studied specices : Mytilus edulis and Crassostrea gigas)
These topics rest on various cellular (classic, transmission and confocal microscopy, cell isolation, laserr-microdissection, immunocytology) and molecular (proteomic, 2DE, transcriptomic, HIS) approaches and also in vivo experiments on field or under controlled conditions (conditioning experiments).
Licence and Master teaching activities consists mainly in:
- Animal Biology Licence in Biology
- Genetic Licence in Biology
- Evolutive and Compared Endocrinology Master 1 Aquacaen
- Physiology of marine organisms Master 1 Aquacaen
- Development of aquatic organisms Master 1 Aquacaen
- Methodology for investigating cell communication and methodology in physiology Master 1 Aquacaen
Participation to manifestations of knowledge braodcasting diffusion (fête de la science, scientific photos exhibition) and representation of the formation offer in Biology in University of Caen-Basse Normandie (Student forum, Carrier forum…).
- Member of driving comity of Licence in Biology
- Responsability of the semestre 3 of the Licence in Biology
- Presidente of jury for the second year of Licence
- Responsability of several teachning unities (L and M)
- Member of comity of selection in 68th section in the UCBN
Cherif–Feildel, Maëva , Clothilde Heude Berthelin, Béatrice Adeline, Guillaume Rivière, Pascal Favrel, and Kristell Kellner-Cousin. 2019. “Molecular Evolution And Functional Characterisation Of Insulin Related Peptides In Molluscs: Contributions Of Crassostrea Gigas Genomic And Transcriptomic-Wide Screening”. Journal Of Comparative Endocrinology 271: 15-29.
Cherif-Feildel, M. , Heude C. Berthelin, G. Rivière, Pascal Favrel, and Kristell Kellner-Cousin. 2019. “Data For Evolutive Analysis Of Insulin Related Peptides In Bilaterian Species.”. Journal Of Comparative Endocrinology 22: 546-550. doi:10.1016/j.dib.2018.12.050. https://doi.org/10.1016/j.dib.2018.12.050.
Cherif‑Feildel, Maëva , Kristell Kellner-Cousin, Didier Goux, Nicolas Elie, Béatrice Adeline, Christophe Lelong, and Clothilde Heude Berthelin. 2018. “Morphological And Molecular Criteria Allow The Identification Of Putative Germ Stem Cells In A Lophotrochozoan, The Pacific Oyster Crassostrea Gigas”. Histochemistry And Cell Biology. doi:https://doi.org/10.1007/s00418-018-1740-3.
Boonmee, A , C Heude-Berthelin, S Kingtong, M Pauletto, B Bernay, B Adeline, M Suquet, Pascal Sourdaine, and Kristell Kellner-Cousin. 2016. “Differential Protein Expression During Sperm Maturation And Capacitation In An Hermaphroditic Bivalve, Pecten Maximus (Linnaeus, 1758)”. Journal Of Molluscan Studies: 1-10.
Mezghani-Chaari, Sawssan , Monia Machreki-Ajmi, Gauthier Tremolet, Kristell Kellner-Cousin, Alain Geffard, Christophe Minier, and Amel Hamza-Chaffai. 2015. “The Endocrine-Disrupting Effect And Other Physiological Responses Of Municipal Effluent On The Clam Ruditapes Decussatus”. Environ Sci Pollut Res Int. 22 (24): 19716-28.
Mezghani-Chaari, Sawssan , Monia Machreki-Ajmi, Gauthier Tremolet, Kristell Kellner-Cousin, Alain Geffard, Christophe Minier, and Amel Hamza-Chaffai. 2015. “The Endocrine-Disrupting Effect And Other Physiological Responses Of Municipal Effluent On The Clam Ruditapes Decussatus.”. Environ Sci Pollut Res Int 22 (24): 19716-28. doi:10.1007/s11356-015-5199-7.