- Pascal SOURDAINE
Key words: Elasmobranchs - Spermatogenesis - Steroidogenesis - Spermatogonial stem cells – Cellular biology
Identification of early markers of spermatogenesis in the small spotted catshark (Scyliorhinus canicula L.).
Spermatogenesis start with the multiplication and differentiation of diploid spermatogonia and ends with the production of highly specialized haploid cells, spermatozoa. Continuous production of male gametes is dependent on the ability of spermatogonial stem cells (SSC) to renew while maintaining their stemness. This takes place within a specific microenvironment called the "niche" and involving autocrine and paracrine factors, these last ones secreted from the surrounding somatic cells. Our studies focus on the identification and functional characterization of the SSC and of the molecular players that control their self-renewal and differentiation using a Chondrichthyans model, the small spotted catshark (Scyliorhinus canicula). The identification of molecular markers is performed through proteomic and transcriptomic approaches on purified cells or testicular areas. Our studies also address the evolution of the niche of germline stem cells in Gnathostomes.
Evolution of GnRH systems and of their regulatory neuropeptides in endocrine and paracrine controls of spermatogenesis in the dogfish Scyliorhinus canicula.
The hypothalamic-pituitary system is the main regulator of reproduction and its evolution was accompanied by a specialization of its regulatory peptides that have tissue distributions and broader functions in invertebrates. These peripheral functions have persisted in vertebrates and their studies are emerging in the context of understanding effects of environmental cues on reproduction. In an evolutionary context, it is relevant to include Chondrichthyes as the sister group of Osteichthyes (bony vertebrates) to better understand the evolution of GnRH systems and of their regulatory neuropeptides (KNDy: Kisspeptin, Neurokinin, Dynorphin; RFRP3, the mammalian orthologue of GnIH). Analyses of genomes from Chondrichthyes have allowed identifying several actors implied in the control of growth, reproduction and energy homeostasis. GnRH and GnRHR genes as well as those of the KNDy network are also expressed in the male reproductive tract, asking about their paracrine function. Our goal is the study of the evolution of those neuropeptides and their involvement in endocrine and paracrine controls of spermatogenesis in the dogfish. The choice of S. canicula is motivated by its phylogenetic position, the organization of its central nervous system and its circulatory system (absence of portal system) and by the relative independence of its spermatogenesis from a gonadotropin regulation.
- Person in charge of the teaching units "Reproduction and Development" (L2), "Biologie du Développement et Régénération" (L3), "Introduction to biotherapy" (M1 Biologie Santé) and "Student Professional Project" (M1 Biologie Santé and M1 AquaCaen).
- Member of the pedagogical and scientific commission (CPS) of the Normand Doctoral School of Integrative Biology, Health and Environment (EdN BISE 497)
- Member of the Regional Ethics Committee on Animal Experiments (CENOMEXA).
- Co-coordinator of the Research and Training Center Continuum Terre Mer of the Normandie University.
- Head of CPER MANCHE 2021 (FEDER FSE 2014/2020 -Région Normandie). This project aims to federate and develop research on societal, food and energy transitions in the Manche area using an integrated transdisciplinary approach.
Bosseboeuf, Adrien , Amandine Baron, Elise Duval, Aude Gautier, Pascal Sourdaine, and Pierrïck Auvray. 2019. “K092A And K092B, Two Peptides Isolated From The Dogfish (Scyliorhinus Canicula L.), With Potential Antineoplastic Activity Against Human Prostate And Breast Cancer Cells”. Marine Drugs 17 (12): 672. doi:10.3390/md17120672. https://www.mdpi.com/1660-3397/17/12/672.
Bosseboeuf, Adrien , Amandine Baron, Elise Duval, Aude Gautier, Pascal Sourdaine, and Pierrïck Auvray. 2019. “A Potential Antineoplastic Peptide Of Human Prostate Cancer Cells Derived From The Lesser Spotted Dogfish (Scyliorhinus Canicula L.)”. Marine Drugs 17 (10): 585. doi:10.3390/md17100585. https://www.mdpi.com/1660-3397/17/10/585.
Sourdaine, Pascal , Aude Gautier, and Laura Gribouval. 2018. “Spermatogenesis And Spermiogenesis In Elasmobranchs, A Short Overview”. In Encyclopedia Of Reproduction, Second Edition, 6:305 - 312. Elsevier. doi:10.1016/B978-0-12-809633-8.20572-4. https://linkinghub.elsevier.com/retrieve/pii/B9780128096338205724.
Gribouval, Laura , Pascal Sourdaine, Jean-Jacques Lareyre, Johanna Bellaiche, Florence Le Gac, Sylvie Mazan, Cécile Guiardiere, Pierrïck Auvray, and Aude Gautier. 2018. “The Nanos1 Gene Was Duplicated In Early Vertebrates And The Two Paralogs Show Different Gonadal Expression Profiles In A Shark”. Scientific Reports 8 (1). doi:10.1038/s41598-018-24643-1. http://www.nature.com/articles/s41598-018-24643-1.
Rivière, Guillaume , Yan He, Samuele Tecchio, Elizabeth Crowell, Michaël Gras, Pascal Sourdaine, Ximing Guo, and Pascal Favrel. 2017. “Dynamics Of Dna Methylomes Underlie Oyster Development”. Plos Genetics 13 (6): e1006807. https:// doi.org/10.13 71/journal.p gen.1006807.
Boonmee, A , C Heude-Berthelin, S Kingtong, M Pauletto, B Bernay, B Adeline, M Suquet, Pascal Sourdaine, and Kristell Kellner-Cousin. 2016. “Differential Protein Expression During Sperm Maturation And Capacitation In An Hermaphroditic Bivalve, Pecten Maximus (Linnaeus, 1758)”. Journal Of Molluscan Studies: 1-10.
Boonmee, A , Heude C Berthelin, S Kingtong, M Pauletto, B Bernay, M Suquet, Pascal Sourdaine, and Kellner K. 2016. “Differential Protein Expression During Sperm Maturation And Capacitation In An Hermaphroditic Bivalve, Pecten Maximus (Linnaeus, 1758)”. J. Of Molluscan Studies 82 (4): 575-584. doi:doi:10.1093/mollus/eyw028.
He, Yan , Aude Jouaux, Susan E Ford, Christophe Lelong, Pascal Sourdaine, Michel Mathieu, and Ximing Guo. 2015. “Transcriptome Analysis Reveals Strong And Complex Antiviral Response In A Mollusc.”. Fish Shellfish Immunol. doi:10.1016/j.fsi.2015.05.023.
Gautier, Aude , A Bosseboeuf, P Auvray, and Pascal Sourdaine. 2014. “Maintenance Of Potential Spermatogonial Stem Cells In Vitro By Gdnf Treatment In A Chondrichthyan Model (Scyliorhinus Canicula L.).”. Biol Reprod 91 (4): 91. doi:10.1095/biolreprod.113.116020.
Bosseboeuf, A , Aude Gautier, P Auvray, S Mazan, and Pascal Sourdaine. 2014. “Characterization Of Spermatogonial Markers In The Mature Testis Of The Dogfish (Scyliorhinus Canicula L.)”. Reproduction 147: 125–139.