Titre | Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas. |
Type de publication | Journal Article |
Year of Publication | 2007 |
Auteurs | Badariotti, F, Lelong, C, Dubos, M-P, Favrel, P |
Journal | FEBS J |
Volume | 274 |
Ticket | 14 |
Pagination | 3646-54 |
Date Published | 2007 Jul |
ISSN | 1742-464X |
Mots-clés | Aging, Amino Acid Sequence, Animals, Chitinase, Crassostrea, DNA, Complementary, Gene Expression Regulation, Developmental, Hemocytes, Humans, Lipopolysaccharides, Molecular Sequence Data, RNA, Messenger, Sequence Alignment, Sequence Homology, Transcription, Genetic |
Résumé | Chitinase-like proteins have been identified in insects and mammals as nonenzymatic members of the glycoside hydrolase family 18. Recently, the first molluscan chitinase-like protein, named Crassostrea gigas (Cg)-Clp1, was shown to control the proliferation and synthesis of extracellular matrix components of mammalian chondrocytes. However, the precise physiological roles of Cg-Clp1 in oysters remain unknown. Here, we report the cloning and the characterization of a new chitinase-like protein (Cg-Clp2) from the oyster Crassostrea gigas. Gene expression profiles monitored by quantitative RT-PCR in adult tissues and through development support its involvement in tissue growth and remodelling. Both Cg-Clp1- and Cg-Clp2-encoding genes were transcriptionally stimulated in haemocytes in response to bacterial lipopolysaccharide challenge, strongly suggesting that these two close paralogous genes play a role in oyster immunity. |
DOI | 10.1111/j.1742-4658.2007.05898.x |
Alternate Journal | FEBS J. |
Identifiant (ID) PubMed | 17608806 |