Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas.

TitreCharacterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas.
Type de publicationJournal Article
Year of Publication2007
AuteursBadariotti, F, Lelong, C, Dubos, M-P, Favrel, P
JournalFEBS J
Volume274
Ticket14
Pagination3646-54
Date Published2007 Jul
ISSN1742-464X
Mots-clésAging, Amino Acid Sequence, Animals, Chitinase, Crassostrea, DNA, Complementary, Gene Expression Regulation, Developmental, Hemocytes, Humans, Lipopolysaccharides, Molecular Sequence Data, RNA, Messenger, Sequence Alignment, Sequence Homology, Transcription, Genetic
Résumé

Chitinase-like proteins have been identified in insects and mammals as nonenzymatic members of the glycoside hydrolase family 18. Recently, the first molluscan chitinase-like protein, named Crassostrea gigas (Cg)-Clp1, was shown to control the proliferation and synthesis of extracellular matrix components of mammalian chondrocytes. However, the precise physiological roles of Cg-Clp1 in oysters remain unknown. Here, we report the cloning and the characterization of a new chitinase-like protein (Cg-Clp2) from the oyster Crassostrea gigas. Gene expression profiles monitored by quantitative RT-PCR in adult tissues and through development support its involvement in tissue growth and remodelling. Both Cg-Clp1- and Cg-Clp2-encoding genes were transcriptionally stimulated in haemocytes in response to bacterial lipopolysaccharide challenge, strongly suggesting that these two close paralogous genes play a role in oyster immunity.

DOI10.1111/j.1742-4658.2007.05898.x
Alternate JournalFEBS J.
Identifiant (ID) PubMed17608806