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Cryopreservation of mantle dissociated cells from Haliotis tuberculata (Gastropoda) and postthawed primary cell cultures.

TitreCryopreservation of mantle dissociated cells from Haliotis tuberculata (Gastropoda) and postthawed primary cell cultures.
Type de publicationJournal Article
Year of Publication2002
AuteursPoncet, J-M, Serpentini, A, Boucaud-Camou, E, Lebel, J-M
JournalCryobiology
Volume44
Ticket1
Pagination38-45
Date Published2002 Feb
ISSN0011-2240
Mots-clésAnimals, Cell Separation, Cell Survival, Cells, Cultured, Cryopreservation, Cryoprotective Agents, Dimethyl Sulfoxide, Glycerol, Insulin, Insulin-Like Growth Factor I, Mollusca, Protein Biosynthesis
Résumé

Dissociated mantle cells from the gastropod mollusc Haliotis tuberculata were cultured after a freezing-thawing procedure using either 10% dimethyl sulfoxide (Me(2)SO) or 10% glycerol (Gly) as a cryoprotector. The survival rate of 2-day-old cultured cryopreserved cells after thawing, based on analysis of DNA and protein contents, was nearly 80% in comparison with 2-day-old cultured fresh cells. Cells thawed after cryopreservation exhibited the maintenance of all tested physiological activities. Metabolic activity (measured by the MTT test) and the activity of alkaline phosphatase (a plasma membrane-bound enzyme) were not decreased in comparison to those in cultured fresh cells. In addition, cryopreserved cultured cells maintained a physiological stimulation ability in response to treatment with growth factors. These results taken together represent one of the most convincing demonstrations of the survival and of the recovery of intact functional activities of molluscan cells after a freeze-thawing procedure. Our results suggest that in the future primary cultures of cryopreserved mantle cells will be able to be used for fundamental research, in toxicity tests, or in the field of biotechnology.

DOI10.1016/S0011-2240(02)00001-9
Alternate JournalCryobiology
Identifiant (ID) PubMed12061846