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Dietary vitamin \D3\ affects digestive system ontogenesis and ossification in European sea bass (Dicentrachus labrax, Linnaeus, 1758)

TitreDietary vitamin \D3\ affects digestive system ontogenesis and ossification in European sea bass (Dicentrachus labrax, Linnaeus, 1758)
Type de publicationJournal Article
Year of Publication2010
AuteursDarias, M, Mazurais, D, Koumoundouros, G, Glynatsi, N, Christodoulopoulou, S, Huelvan, C, Desbruyeres, E, Le Gall, MM, Quazuguel, P, Cahu, CL, Zambonino-Infante, J
JournalAquaculture
Volume298
Pagination300 - 307
ISSN0044-8486
Mots-clésGene Expression
Résumé

The influence of dietary vitamin \{D3\} (VD3) on survival, growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45 days post hatching. Diets contained 4% of the standard vitamin mix (VM) recommended by the National Research Council (NRC) and incorporated 0, 19.2, 38.4, or 140 İU\} of \{VD3\} per gram of diet to give VD-0, VD-1, VD-2 and VD-3 dietary treatments, respectively. The present study revealed for the first time an impact of dietary \{VD3\} on the sea bass digestive system ontogenesis that consequently conditioned the ossification process and morphogenesis. All dietary \{VD3\} levels were in the “adequate range” based on larval survival. Nevertheless, growth, intestinal maturation and ossification at the end of the larval stage were harmed by the minimum dose of \{VD3\} tested and resulted in the appearance of malformations. VD-2 and VD-3 groups showed satisfactory growth and ossification levels at the end of the larval period. However, results of enzymatic activity and expression of genes involved in the \{VD3\} pathway (bone morphogenetic protein 4, osteocalcin, vitamin D receptors and transient receptor potential cation channel-subfamily V, member 6-) gave evidence of complications during the ossification process as revealed by the high percentage of deformed larvae. A \{VD3\} level of 19.2 IU/g diet appeared necessary to obtain harmonious larval morphogenesis.

URLhttp://www.sciencedirect.com/science/article/pii/S0044848609008904
DOI10.1016/j.aquaculture.2009.11.002