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Identification of Two Carbonic Anhydrases in the Mantle of the European Abalone Haliotis tuberculata (Gastropoda, Haliotidae): Phylogenetic Implications

TitreIdentification of Two Carbonic Anhydrases in the Mantle of the European Abalone Haliotis tuberculata (Gastropoda, Haliotidae): Phylogenetic Implications
Type de publicationJournal Article
Year of Publication2012
AuteursLe Roy, N, Marie, B, Gaume, B, Guichard, N, DELGADO, SIDNEY, Zanella-Cléon, I, Becchi, M, Auzoux-Bordenave, S, Sire, J-Y, Marin, F
JournalJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume318
Pagination353–367
ISSN1552-5015
Résumé

Carbonic anhydrases (CAs) represent a diversified family of metalloenzymes that reversibly catalyze the hydration of carbon dioxide. They are involved in a wide range of functions, among which is the formation of CaCO3 skeletons in metazoans. In the shell-forming mantle tissues of mollusks, the location of the CA catalytic activity is elusive and gives birth to contradicting views. In the present paper, using the European abalone Haliotis tuberculata, a key model gastropod in biomineralization studies, we identified and characterized two CAs (htCA1 and htCA2) that are specific of the shell-forming mantle tissue. We analyzed them in a phylogenetic context. Combining various approaches, including proteomics, activity tests, and in silico analyses, we showed that htCA1 is secreted but is not incorporated in the organic matrix of the abalone shell and that htCA2 is transmembrane. Together with previous studies dealing with molluskan CAs, our findings suggest two possible modes of action for shell mineralization: the first mode applies to, for example, the bivalves Unio pictorum and Pinctada fucata, and involves a true CA activity in their shell matrix; the second mode corresponds to, for example, the European abalone, and does not include CA activity in the shell matrix. Our work provides new insight on the diversity of the extracellular macromolecular tools used for shell biomineralization study in mollusks. J. Exp. Zool. (Mol. Dev. Evol.) 318B:353-367, 2012. © 2012 Wiley Periodicals, Inc.

URLhttp://dx.doi.org/10.1002/jez.b.22452
DOI10.1002/jez.b.22452