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KKKKPLFGLFFGLF: a cationic peptide designed to exert antibacterial activity.

TitreKKKKPLFGLFFGLF: a cationic peptide designed to exert antibacterial activity.
Type de publicationJournal Article
Year of Publication2009
AuteursDuval, E, Zatylny-Gaudin, C, Laurencin, M, Baudy-Floc'h, M, Henry, J
JournalPeptides
Volume30
Ticket9
Pagination1608-12
Date Published2009 Sep
ISSN1873-5169
Mots-clésAnimals, Antimicrobial Cationic Peptides, bacteria, Cell Membrane, Cell Proliferation, Cell Survival, CHO Cells, Cricetinae, Cricetulus, Databases, Protein, Drug Design, Escherichia coli, Gram-Negative Bacteria, Gram-Positive Bacteria, Hemolysis, Rabbits, Spectrometry, Mass, Electrospray Ionization
Résumé

With 14 residues organized as two domains linked by a single proline, the de novo peptide called K4 was designed, using Antimicrobial Peptide Database, to exert antibacterial activity. The N-terminal domain is composed of four lysines enhancing membrane interactions, and the C-terminal domain is putatively folded into a hydrophobic alpha-helix. Following the synthesis, the purification and the structural checking, antibacterial assays revealed a strong activity against gram-positive and gram-negative bacteria including human pathogenic bacteria such as Staphylococcus aureus and some marine bacteria of the genus Vibrio. Scanning electron microscopy of Escherichia coli confirmed that K4 lyses bacterial cells. The cytotoxicity was tested against rabbit erythrocytes and chinese hamster ovary cells (CHO-K1). These tests revealed that K4 is non-toxic to mammalian cells for bacteriolytic concentrations. The peptide K4 could be a valuable candidate for future therapeutic applications.

DOI10.1016/j.peptides.2009.06.022
Alternate JournalPeptides
Identifiant (ID) PubMed19573572